公开(公告)号：US20240294648A1

公开(公告)日：2024-09-05

申请号：US18473695

申请日：2023-09-25

Applicant: The Johns Hopkins University

Inventor： Emily Han-Chung Hsiue ,  Qing Wang ,  Bert Vogelstein ,  Kenneth W. Kinzler ,  Shibin Zhou ,  Jacqueline Douglass ,  Michael S. Hwang ,  Nickolas Papadopoulos

IPC: C07K16/28 ,  A61K35/17 ,  A61K38/00 ,  C07K14/705 ,  C07K14/725 ,  C07K14/73

CPC classification number: C07K16/2833 ,  A61K35/17 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/70517 ,  C07K14/70521 ,  C07K16/2809 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/622 ,  C07K2319/03

Abstract: This document provides methods and materials for assessing a mammal having or suspected of having cancer and/or for treating a mammal having cancer. For example, molecules including one or more antigen-binding domains (e.g., a single-chain variable fragment (scFv)) that can bind to a modified peptide (e.g., a tumor antigen), as well as method for using such molecules, are provided.

公开(公告)号：US20200079854A1

公开(公告)日：2020-03-12

申请号：US16614005

申请日：2018-05-16

Applicant: The Johns Hopkins University

Inventor： Emily Han-Chung Hsiue ,  Qing Wang ,  Bert Vogelstein ,  Kenneth W. Kinzler ,  Shibin Zhou ,  Jacqueline Douglass ,  Michael S. Hwang ,  Nickolas Papadopoulos

IPC: C07K16/28 ,  A61K35/17 ,  C07K14/73 ,  C07K14/705 ,  C07K14/725

Abstract: This document provides methods and materials for assessing a mammal having or suspected of having cancer and/or for treating a mammal having cancer. For example, molecules including one or more antigen-binding domains (e.g., a single-chain variable fragment (scFv)) that can bind to a modified peptide (e.g., a tumor antigen), as well as method for using such molecules, are provided.

公开(公告)号：US11807662B2

公开(公告)日：2023-11-07

申请号：US16614005

申请日：2018-05-16

Applicant: The Johns Hopkins University

Inventor： Emily Han-Chung Hsiue ,  Qing Wang ,  Bert Vogelstein ,  Kenneth W. Kinzler ,  Shibin Zhou ,  Jacqueline Douglass ,  Michael S. Hwang ,  Nickolas Papadopoulos

IPC: A61K39/395 ,  C07K16/28 ,  A61K35/17 ,  C07K14/725 ,  C07K14/73 ,  C07K14/705 ,  A61K38/00

CPC classification number: C07K16/2833 ,  A61K35/17 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/70517 ,  C07K14/70521 ,  C07K16/2809 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/622 ,  C07K2319/03

Abstract: This document provides methods and materials for assessing a mammal having or suspected of having cancer and/or for treating a mammal having cancer. For example, molecules including one or more antigen-binding domains (e.g., a single-chain variable fragment (scFv)) that can bind to a modified peptide (e.g., a tumor antigen), as well as method for using such molecules, are provided.

公开(公告)号：US20240166751A1

公开(公告)日：2024-05-23

申请号：US18351087

申请日：2023-07-12

Applicant: The Johns Hopkins University

Inventor： Bert Vogelstein ,  Kenneth W. Kinzler ,  Shibin Zhou ,  Luis Diaz ,  Nickolas Papadopoulos ,  Andrew Skora ,  Jacqueline Douglass ,  Michael S. Hwang

IPC: C07K16/28 ,  C07K7/00 ,  C07K14/47 ,  C07K14/71 ,  C07K14/82 ,  C07K16/00 ,  C07K16/18 ,  C07K16/32 ,  C07K16/40 ,  C07K19/00 ,  G01N33/574 ,  G01N33/68

CPC classification number: C07K16/2833 ,  C07K7/00 ,  C07K14/47 ,  C07K14/4746 ,  C07K14/71 ,  C07K14/82 ,  C07K16/005 ,  C07K16/18 ,  C07K16/2863 ,  C07K16/32 ,  C07K16/40 ,  C07K19/00 ,  G01N33/574 ,  G01N33/6854 ,  C07K2317/24 ,  C07K2317/32 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/734 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/03 ,  C07K2319/41 ,  C07K2319/50 ,  G01N2333/7051 ,  G01N2333/70539

Abstract: Mutant epitopes encoded by cancer genes are virtually always located in the interior of cells, making them invisible to conventional antibodies. We generated single chain variable fragments (scFvs) specific for mutant peptides presented on the cell surface by human leukocyte antigen (HLA) molecules. These scFvs can be converted to full-length antibodies, termed MANAbodies, targeting “Mutation Associated Neo-Antigens” bound to HLA. A phage display library representing a highly diverse array of single-chain variable fragment sequences was first designed and constructed. A competitive selection protocol was then used to identify clones specific for peptides bound to pre-defined HLA types. In this way, we obtained scFvs, including one specific for a peptide encoded by a common KRAS mutant and another by a common EGFR mutant. Molecules targeting MANA can be developed that specifically react with mutant peptide-HLA complexes even when these peptides differ by only one amino acid from the normal, wild-type form.

公开(公告)号：US20240148870A1

公开(公告)日：2024-05-09

申请号：US18281471

申请日：2022-03-11

Applicant: The Johns Hopkins University

Inventor： Michael S. Hwang ,  Jacqueline Douglass ,  Emily Han-Chung Hsiue ,  Kenneth W. Kinzler ,  Brian J. Mog ,  Nickolas Papadopoulos ,  Alexander H. Pearlman ,  Bert Vogelstein ,  Shibin Zhou

IPC: A61K39/00 ,  A61P35/00 ,  C07K14/705 ,  C07K14/725 ,  C07K16/28 ,  C12N5/0783

CPC classification number: A61K39/464412 ,  A61K39/4611 ,  A61K39/4631 ,  A61P35/00 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70521 ,  C07K16/2803 ,  C07K16/2833 ,  C12N5/0636 ,  A61K2239/21 ,  A61K2239/22 ,  A61K2239/28 ,  C07K2317/53 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/03 ,  C12N2510/00

Abstract: Provided herein are immune cells and methods of use, wherein the immune cells include a chimeric antigen receptor (CAR), wherein the CAR comprises an extracellular antigen binding domain that binds specifically to a first epitope; and an inhibitory chimeric antigen receptor (iCAR), wherein the iCAR comprises an extracellular antigen binding domain that binds specifically to a second epitope, wherein the immune cell is activated when the immune cells binds to the first epitope and does not bind to the second epitope; and wherein the immune cell is inactivated when the immune cell binds to the first and second epitopes.

公开(公告)号：US20240002541A1

公开(公告)日：2024-01-04

申请号：US18039403

申请日：2021-12-01

Applicant: The Johns Hopkins University

Inventor： Sarah DiNapoli ,  Jacqueline Douglass ,  Emily Han-Chung Hsiue ,  Michael S. Hwang ,  Kenneth W. Kinzler ,  Maximilian Konig ,  Brian J. Mog ,  Nickolas Papadopoulos ,  Andrew M. Pardoll ,  Suman Paul ,  Alexander H. Pearlman ,  Bert Vogelstein ,  Shibin Zhou

IPC: C07K16/46 ,  A61P35/00

CPC classification number: C07K16/468 ,  A61P35/00 ,  C07K2317/565 ,  C07K2317/31

Abstract: This document relates to methods and materials for treating T cell cancers. For example, a composition containing one or more bispecific molecules can be administered to a mammal having a T cell cancer to treat the mammal. For example, methods and materials for using one or more bispecific molecules to treat a mammal having a T cell cancer are provided.

公开(公告)号：US20230051847A1

公开(公告)日：2023-02-16

申请号：US17783506

申请日：2020-12-17

Applicant: The Johns Hopkins University

Inventor： Bert Vogelstein ,  Kenneth W. Kinzler ,  Emily Han-Chung Hsiue ,  Jacqueline Douglass ,  Michael S. Hwang ,  Alexander Pearlman ,  Nickolas Papadopoulos ,  Shibin Zhou ,  Brian Mog ,  Katharine M. Wright ,  Sandra B. Gabelli

IPC: C07K16/30 ,  C07K16/28 ,  A61P35/00

Abstract: This document provides methods and materials for assessing a mammal having or suspected of having cancer and/or for treating a mammal having cancer. For example, molecules including one or more antigen-binding domains (e.g., a single-chain variable fragment (scFv)) that can bind to a modified peptide (e.g., a tumor antigen), as well as method for using such molecules, are provided.