公开(公告)号：US11472849B2

公开(公告)日：2022-10-18

申请号：US16283380

申请日：2019-02-22

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander

IPC: C12N15/10 ,  C12P19/34 ,  C12P21/02 ,  C07K14/195 ,  C12N15/66 ,  C40B40/08 ,  C40B50/06 ,  C12N9/22 ,  C12Q1/6806

Abstract: The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and second sets.

公开(公告)号：US10273271B2

公开(公告)日：2019-04-30

申请号：US15156574

申请日：2016-05-17

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/66 ,  C12P19/34 ,  C40B40/08 ,  C40B50/06 ,  C12P21/02 ,  C07K14/195 ,  C12Q1/6806

Abstract: The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and second sets.

公开(公告)号：US20160318978A1

公开(公告)日：2016-11-03

申请号：US15156574

申请日：2016-05-17

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander

IPC: C07K14/195 ,  C12N9/22 ,  C12N15/10 ,  C12P19/34

CPC classification number: C07K14/195 ,  C07K2319/00 ,  C07K2319/24 ,  C07K2319/41 ,  C07K2319/43 ,  C07K2319/80 ,  C12N9/22 ,  C12N15/1093 ,  C12N15/66 ,  C12P19/34 ,  C12P21/02 ,  C12Q1/6806 ,  C12Y301/00 ,  C40B40/08 ,  C40B50/06 ,  C12Q2521/501 ,  C12Q2563/149

Abstract: The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and second sets.

Abstract translation: 本公开描述了包括提供具有编码包含一个或多个转录激活子样效应子（TALE）重复结构域和/或一个或多个TALE重复结构域的一个或多个部分的第一组的序列的第一核酸的方法; 使所述第一核酸与第一酶接触，其中所述第一酶产生第一可结合末端; 提供具有编码包含一个或多个TALE重复结构域和/或一个或多个TALE重复结构域的一个或多个部分的第二组的序列的第二核酸; 使所述第二核酸与第二酶接触，其中所述第二酶产生第二可连接末端，并且其中所述第一和第二可连接末端是相容的; 以及通过所述第一和第二可连接末端连接所述第一和第二核酸以产生第一连接的核酸，其中所述第一连接的核酸与固体支持物连接，并且其中所述第一连接的核酸编码包含所述第一和第二核酸的多肽 第二套

公开(公告)号：US20230407341A1

公开(公告)日：2023-12-21

申请号：US18178675

申请日：2023-03-06

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander ,  Morgan Maeder ,  Yanfang Fu

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N9/22 ,  C12N15/63 ,  C12Y301/21004 ,  C12N15/85 ,  C07K14/005 ,  C07K14/195 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01 ,  C12N15/102 ,  C12N15/1031 ,  C12N15/11 ,  C07K2319/00 ,  C07K2319/01 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00 ,  C12N2800/80 ,  C12N2310/20 ,  C07K2319/80

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).

公开(公告)号：US11634731B2

公开(公告)日：2023-04-25

申请号：US16572248

申请日：2019-09-16

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander ,  Morgan Maeder ,  Yanfang Fu

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).

公开(公告)号：US10138493B2

公开(公告)日：2018-11-27

申请号：US15686419

申请日：2017-08-25

Applicant: TRUSTEES OF BOSTON UNIVERSITY ,  THE GENERAL HOSPITAL CORPORATION

Inventor： Ahmad S. Khalil ,  Divya Israni ,  Minhee Park ,  J. Keith Joung ,  Jeffry D. Sander

IPC: C12N15/85 ,  C07K14/47 ,  C07K14/00 ,  C12N15/113

Abstract: Embodiments disclosed herein provide artificial expression systems comprising the zinc-finger containing transcription factors and engineered promoters to modulate expression of genes of interest. Engineered zinc-finger transcription factors that interact with engineered promoters constitute synthetic and regulatable expression systems which facilitate the modulation of gene expression as desired.

公开(公告)号：US10119133B2

公开(公告)日：2018-11-06

申请号：US14775930

申请日：2014-03-14

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander ,  Yan-fang Fu ,  Morgan Maeder

IPC: C07H21/02 ,  C12N15/00 ,  A61K48/00 ,  C12N15/10 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01

Abstract: CRISPR-Cas genome editing uses a guide RNA, which includes both a complementarity region, which binds the target DNA by base-pairing, and a Cas9-binding region, to direct a Cas9 nuclease to a target DNA. Further disclosed are methods for increasing specificity of RNA-guided genome editing using CRISPR/Cas9 systems by using truncated guide RNAs (tru-gRNAs).

公开(公告)号：US20210130850A1

公开(公告)日：2021-05-06

申请号：US17099503

申请日：2020-11-16

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  James Angstman ,  Jeffry D. Sander ,  Morgan Maeder ,  Shengdar Tsai

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.

公开(公告)号：US10415059B2

公开(公告)日：2019-09-17

申请号：US15430218

申请日：2017-02-10

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander ,  Morgan Maeder ,  Yanfang Fu

IPC: C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12N15/90 ,  C07K14/005 ,  C07K14/195

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).

公开(公告)号：US20190177374A1

公开(公告)日：2019-06-13

申请号：US16283380

申请日：2019-02-22

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander

IPC: C07K14/195 ,  C12N15/10 ,  C12N15/66 ,  C40B40/08 ,  C40B50/06 ,  C12P21/02 ,  C12P19/34 ,  C12N9/22

Abstract: The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and second sets.