公开(公告)号：US12152254B2

公开(公告)日：2024-11-26

申请号：US17149458

申请日：2021-01-14

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Morgan Maeder ,  James Angstman

IPC: C12N9/02

Abstract: Fusion proteins comprising a DNA binding domain, e.g., a TAL effector repeat array or zinc finger, and a catalytic domain comprising a sequence that catalyzes hydroxylation of methylated cytosines in DNA, and methods of use thereof.

公开(公告)号：US20220090145A1

公开(公告)日：2022-03-24

申请号：US17450135

申请日：2021-10-06

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Morgan Maeder

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11

Abstract: Methods and constructs for RNA-guided targeting of heterologous functional domains such as transcriptional activators to specific genomic loci.

公开(公告)号：US10378027B2

公开(公告)日：2019-08-13

申请号：US14775869

申请日：2014-03-14

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Morgan Maeder

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C07K14/00 ,  C07K14/195 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11 ,  C07K14/005 ,  C12N9/02 ,  C12N9/10

Abstract: Methods and constructs for RNA-guided targeting of heterologous functional domains such as transcriptional activators to specific genomic loci.

公开(公告)号：US20180187168A1

公开(公告)日：2018-07-05

申请号：US15894004

申请日：2018-02-12

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Morgan Maeder ,  James Angstman

IPC: C12N9/02

CPC classification number: C12N9/0071 ,  C07K2319/80 ,  C07K2319/81

Abstract: Fusion proteins comprising a DNA binding domain, e.g., a TAL effector repeat array or zinc finger, and a catalytic domain comprising a sequence that catalyzes hydroxylation of methylated cytosines in DNA, and methods of use thereof.

公开(公告)号：US20210130850A1

公开(公告)日：2021-05-06

申请号：US17099503

申请日：2020-11-16

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  James Angstman ,  Jeffry D. Sander ,  Morgan Maeder ,  Shengdar Tsai

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.

公开(公告)号：US10415059B2

公开(公告)日：2019-09-17

申请号：US15430218

申请日：2017-02-10

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander ,  Morgan Maeder ,  Yanfang Fu

IPC: C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12N15/90 ,  C07K14/005 ,  C07K14/195

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).

公开(公告)号：US09567604B2

公开(公告)日：2017-02-14

申请号：US14213723

申请日：2014-03-14

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Jeffry D. Sander ,  Morgan Maeder ,  Yanfang Fu

IPC: C07H21/02 ,  C12N15/00 ,  A61K48/00 ,  C12N15/85 ,  C12N9/22 ,  C12N15/63 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/96 ,  C12N15/01

CPC classification number: C12N15/102 ,  C07K14/005 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/80 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12N2310/20 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12N2800/80 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00 ,  C12Y301/21004

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems, using truncated guide RNAs (tru-gRNAs).

Abstract translation: 使用截短的引导RNA（tru-gRNA）增加RNA指导基因组编辑的特异性的方法，例如使用CRISPR / Cas9系统的编辑。

公开(公告)号：US20150376626A1

公开(公告)日：2015-12-31

申请号：US14766713

申请日：2014-02-07

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： J. Keith Joung ,  Morgan Maeder

IPC: C12N15/63 ,  G06F19/18 ,  C07K14/435 ,  G01N33/53 ,  C07K14/195

Abstract: Computer programs, algorithms, and methods for identifying TALE-activator binding sites, and methods for generation and use of TALE-activators that bind to said sites.

Abstract translation: 用于鉴定TALE-激活物结合位点的计算机程序，算法和方法，以及用于产生和使用结合所述位点的TALE-活化剂的方法。

公开(公告)号：US20210155907A1

公开(公告)日：2021-05-27

申请号：US17149458

申请日：2021-01-14

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Morgan Maeder ,  James Angstman

IPC: C12N9/02

Abstract: Fusion proteins comprising a DNA binding domain, e.g., a TAL effector repeat array or zinc finger, and a catalytic domain comprising a sequence that catalyzes hydroxylation of methylated cytosines in DNA, and methods of use thereof.

公开(公告)号：US10731167B2

公开(公告)日：2020-08-04

申请号：US15402805

申请日：2017-01-10

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  Morgan Maeder

IPC: G16B20/00 ,  C07K14/195 ,  C07K14/435 ,  C07K14/005 ,  C07K14/47 ,  C12N15/63 ,  C12N15/67 ,  G01N33/53

Abstract: Computer programs, algorithms, and methods for identifying TALE-activator binding sites, and methods for generation and use of TALE-activators that bind to these sites.