公开(公告)号：US20190271033A1

公开(公告)日：2019-09-05

申请号：US16277972

申请日：2019-02-15

Applicant: Takara Bio USA, Inc.

Inventor： Emmanuel KAMBEROV ,  Tong SUN ,  Eric BRUENING ,  Jonathon H. PINTER ,  Irina SLEPTSOVA ,  Takao KURIHARA ,  Vladimir L. MAKAROV

IPC: C12Q1/6853 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6876 ,  C12N15/10

Abstract: The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.

公开(公告)号：US20190323062A1

公开(公告)日：2019-10-24

申请号：US16465008

申请日：2018-04-11

Applicant: Nathalie BOLDUC ,  TAKARA BIO USA, INC.

Inventor： Nathalie BOLDUC ,  Marta GONZALEZ-HERNANDEZ ,  Emmanuel KAMBEROV ,  Brian WALSH

IPC: C12Q1/6806 ,  C12N15/10

Abstract: Provided are methods for generating strand specific nucleic acids. The subject methods may include coupling an adaptor to a 3′-end of a target nucleic acid to form an adaptor-coupled target nucleic acid, which may be combined with further components in a template switching reaction to produce a product nucleic acid. The subject methods find use in a variety of applications, including but not limited to e.g., the preparation of nucleic acid libraries.

公开(公告)号：US20170342475A1

公开(公告)日：2017-11-30

申请号：US15674468

申请日：2017-08-10

Applicant: Takara Bio USA, Inc.

Inventor： Vladimir L. MAKAROV ,  Emmanuel KAMBEROV ,  Brendan J. TARRIER

IPC: C12Q1/68 ,  C12P19/34 ,  C12N15/10

CPC classification number: C12Q1/6853 ,  C12N15/1068 ,  C12P19/34 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q2525/301 ,  C12Q2525/119 ,  C12Q2521/301 ,  C12Q2525/191 ,  C12Q2521/501

Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.

公开(公告)号：US20210292828A1

公开(公告)日：2021-09-23

申请号：US17326844

申请日：2021-05-21

Applicant: Takara Bio USA, Inc.

Inventor： Vladimir L. MAKAROV ,  Emmanuel KAMBEROV ,  Brendan J. TARRIER

IPC: C12Q1/6853 ,  C12P19/34 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12N15/10 ,  C12Q1/686

Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.

公开(公告)号：US20190119741A1

公开(公告)日：2019-04-25

申请号：US16231002

申请日：2018-12-21

Applicant: Takara Bio USA, Inc.

Inventor： Vladimir L. MAKAROV ,  Emmanuel KAMBEROV ,  Brendan J. TARRIER

IPC: C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6865 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/6855

Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.

公开(公告)号：US20180030522A1

公开(公告)日：2018-02-01

申请号：US15674481

申请日：2017-08-10

Applicant: Takara Bio USA, Inc.

Inventor： Emmanuel KAMBEROV ,  Tong SUN ,  Eric BRUENING ,  Jonathon H. PINTER ,  Irina SLEPTSOVA ,  Takao KURIHARA ,  Vladimir L. MAKAROV

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6853 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2525/179 ,  C12Q2525/15

Abstract: The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.