公开(公告)号：US20170342475A1

公开(公告)日：2017-11-30

申请号：US15674468

申请日：2017-08-10

Applicant: Takara Bio USA, Inc.

Inventor： Vladimir L. MAKAROV ,  Emmanuel KAMBEROV ,  Brendan J. TARRIER

IPC: C12Q1/68 ,  C12P19/34 ,  C12N15/10

CPC classification number: C12Q1/6853 ,  C12N15/1068 ,  C12P19/34 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q2525/301 ,  C12Q2525/119 ,  C12Q2521/301 ,  C12Q2525/191 ,  C12Q2521/501

Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.

公开(公告)号：US20210292828A1

公开(公告)日：2021-09-23

申请号：US17326844

申请日：2021-05-21

Applicant: Takara Bio USA, Inc.

Inventor： Vladimir L. MAKAROV ,  Emmanuel KAMBEROV ,  Brendan J. TARRIER

IPC: C12Q1/6853 ,  C12P19/34 ,  C12Q1/6855 ,  C12Q1/6865 ,  C12N15/10 ,  C12Q1/686

Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.

公开(公告)号：US20190119741A1

公开(公告)日：2019-04-25

申请号：US16231002

申请日：2018-12-21

Applicant: Takara Bio USA, Inc.

Inventor： Vladimir L. MAKAROV ,  Emmanuel KAMBEROV ,  Brendan J. TARRIER

IPC: C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6865 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/6855

Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.