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    ANIMAL CELL CULTURE MEDIUM AND CULTURE CONTAINER
    3.
    发明申请
    ANIMAL CELL CULTURE MEDIUM AND CULTURE CONTAINER 审中-公开
    动物细胞培养基和培养容器

    公开(公告)号：US20170022477A1

    公开(公告)日：2017-01-26

    申请号：US15288585

    申请日：2016-10-07

    申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.

    发明人： Kyohei Ota ,  Satoshi Tanaka

    IPC分类号： C12N5/078 ,  C12M1/04

    CPC分类号： C12N5/0634 ,  C12M1/00 ,  C12M23/24 ,  C12N1/00 ,  C12N2500/32

    摘要： A culture medium for animal cells for enhancing a metabolism activity of the TCA cycle (tricarboxylic acid cycle) in respiration of the animal cells includes, as effective components, amino acids metabolized to succinyl CoA that is an element constituting the TCA cycle at a concentration of 2.3 mmol/L to 6.0 mmol/L.

    摘要翻译： 动物细胞在动物细胞的呼吸增强TCA循环的代谢活性（三羧酸循环）的培养基包括，作为有效成分，氨基酸代谢为琥珀酰辅酶A是构成TCA循环中的浓度的元素 2.3 mmol / L至6.0 mmol / L。

    Cell collection method
    4.
    发明授权
    Cell collection method 有权
    细胞收集方法

    公开(公告)号：US09574168B2

    公开(公告)日：2017-02-21

    申请号：US14341543

    申请日：2014-07-25

    申请人： Toyo Seikan Group Holdings, Ltd.

    发明人： Satoshi Tanaka ,  Ryo Suenaga ,  Masahiro Kuninori ,  Kyohei Ota

    IPC分类号： C12N1/02 ,  C12M1/00 ,  C12M1/26

    CPC分类号： C12N1/02 ,  C12M23/14 ,  C12M33/18

    摘要： A method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit is provided. The method includes transferring a culture supernatant that has been cultured from the culture container to the waste liquid container, transferring a concentrated suspension of cells from the culture container to the cell collection container, returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells, and transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell collection container.

    摘要翻译： 采用细胞培养试剂盒收集培养细胞的方法，其中至少培养细胞用培养容器，栽培后收集细胞的细胞收集容器和培养后收集培养基的废液容器， 提供了通过导管彼此连接的。 该方法包括将从培养容器培养的培养上清液转移到废液容器，将细胞浓缩悬浮液从培养容器转移到细胞收集容器中，将排出到废液容器中的部分培养上清液返回到 将培养容器保持在培养容器中的细胞悬浮在细胞悬浮液中，再次将残留的细胞从培养容器悬浮的细胞悬浮液转移到细胞收集容器。

    BUBBLE REMOVAL METHOD AND BUBBLE REMOVAL DEVICE
    8.
    发明申请
    BUBBLE REMOVAL METHOD AND BUBBLE REMOVAL DEVICE 审中-公开
    泡沫拆卸方法和泡沫拆卸装置

    公开(公告)号：US20150191262A1

    公开(公告)日：2015-07-09

    申请号：US14667179

    申请日：2015-03-24

    申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.

    发明人： Ryo Suenaga ,  Satoshi Tanaka ,  Kyohei Ota

    IPC分类号： B65B9/04

    CPC分类号： B65B9/042 ,  B01L3/502723 ,  B01L3/505 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2400/0481 ,  B65B31/00 ,  C12M23/14 ,  C12M41/36

    摘要： Bubbles are removed from an observation area when observing the inside of a container that is made of a soft package material and encloses a liquid containing bubbles. Provided is a method in which, when observing the inside of a container that is made of a soft package material and encloses a liquid that contains bubbles, a part of space in the container is set to be an observation area, and the bubbles are pushed outside the observation area by pressing from above a part of the upper surface of the container that is positioned above the observation area by means of a pressing member and by moving the pressing member.

    摘要翻译： 当观察由软包装材料制成的容器的内部并且包围含有气泡的液体时，从观察区域去除气泡。 提供了一种方法，当观察由软包装材料制成的容器的内部并且包围含有气泡的液体时，容器中的一部分空间被设定为观察区域，并且气泡被推动 通过借助于按压部件从位于观察区域上方的容器的上表面的一部分的上方按压并通过移动按压部件而在观察区域的外侧。

    CELL COLLECTION METHOD
    9.
    发明申请
    CELL COLLECTION METHOD 有权
    细胞收集方法

    公开(公告)号：US20140335600A1

    公开(公告)日：2014-11-13

    申请号：US14341543

    申请日：2014-07-25

    申请人： TOYO SEIKAN GROUP HOLDINGS, LTD.

    发明人： Satoshi Tanaka ,  Ryo Suenaga ,  Masahiro Kuninori ,  Kyohei Ota

    IPC分类号： C12N1/02

    CPC分类号： C12N1/02 ,  C12M23/14 ,  C12M33/18

    摘要： A method for collecting cells that have been cultured by using a cell culture kit in which, at least, a culture container for cultivating cells, a cell collection container for collecting cells after cultivation and a waste liquid container for collecting a culture medium after cultivation are linked to one another through a conduit, the method including transferring a culture supernatant that has been cultured from the culture container to the waste liquid container, transferring a concentrated suspension of cells from the culture container to the cell collection container, returning part of the culture supernatant discharged in the waste liquid container to the culture container and allowing cells remaining in the culture container to be suspended in a suspension of cells, and transferring again a suspension of cells in which remaining cells are suspended from the culture container to the cell collection container.

    摘要翻译： 采用细胞培养试剂盒收集培养细胞的方法，其中至少培养细胞用培养容器，栽培后收集细胞的细胞收集容器和培养后收集培养基的废液容器， 该方法包括将从培养容器培养的培养上清液转移到废液容器，将细胞浓缩悬浮液从培养容器转移到细胞收集容器中，返回部分培养物 将废液容器中的上清液排出到培养容器中，使残留在培养容器中的细胞悬浮于细胞悬浮液中，再次将剩余细胞从培养容器悬浮的细胞悬浮液转移到细胞收集容器 。