公开(公告)号：US20150192510A1

公开(公告)日：2015-07-09

申请号：US14409397

申请日：2013-06-24

Applicant: THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE

Inventor： Rafael Piestun ,  Anurag Agrawal ,  Sean Quirin ,  Anthony Barsic ,  Ginni Grover

IPC: G01N15/14 ,  G01B21/16 ,  G01B11/00 ,  G01B11/14

CPC classification number: G01N15/1429 ,  G01B11/002 ,  G01B11/14 ,  G01B21/16 ,  G01N15/1456 ,  G01N21/6458 ,  G01N2015/0038 ,  G01N2015/1445

Abstract: Imaging or measurement methods and systems including methods and systems for finding the three-dimensional orientation and position of multiple dipole-like particles and single molecules, methods and systems for generating helical beams and helical spread functions, and methods and systems for super-resolution and super-localization of dense arrays of emitters.

Abstract translation: 成像或测量方法和系统，包括用于发现多个偶极子颗粒和单分子的三维取向和位置的方法和系统，用于产生螺旋波束和螺旋扩散函数的方法和系统，以及用于超分辨率和 密集阵列发射体的超定位。

公开(公告)号：US10955331B2

公开(公告)日：2021-03-23

申请号：US14409397

申请日：2013-06-24

Applicant: THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE

Inventor： Rafael Piestun ,  Anurag Agrawal ,  Sean Quirin ,  Anthony Barsic ,  Ginni Grover

IPC: G01N15/14 ,  G01B11/00 ,  G01B11/14 ,  G01B21/16 ,  G01N21/64 ,  G01N15/00

Abstract: Imaging or measurement methods and systems including methods and systems for finding the three-dimensional orientation and position of multiple dipole-like particles and single molecules, methods and systems for generating helical beams and helical spread functions, and methods and systems for super-resolution and super-localization of dense arrays of emitters.

公开(公告)号：US10215975B2

公开(公告)日：2019-02-26

申请号：US15018684

申请日：2016-02-08

Applicant: The Regents of the University of Colorado, a body corporate

Inventor： Rafael Piestun ,  Wyatt Mohrman ,  Ginni Grover

IPC: G02B21/16 ,  G02B21/36 ,  G02B21/12 ,  G02B21/24

Abstract: Some embodiments of the invention include a system comprising a positioning device configured to a hold a sample and adjust a position of a sample in response to receiving a drift compensation signal; a first light source disposed to transilluminate the sample; a second light source disposed to epi-illuminate the sample; an optical system configured to receive light from the sample and generate a three-dimensional point spread function from the light from the sample; an image sensor disposed relative to the optical system that produces an image from the light collected from the sample via the optical system; and logic electrically coupled with the image detector and the positioning device, the logic configured to determine one or more drift compensation values from images imaged by the image detector, and configured to send one or more drift compensation signals to the positioning device.

公开(公告)号：US20160231553A1

公开(公告)日：2016-08-11

申请号：US15018684

申请日：2016-02-08

Applicant: The Regents of the University of Colorado, a body corporate

Inventor： Rafael Piestun ,  Wyatt Mohrman ,  Ginni Grover

IPC: G02B21/36 ,  G02B21/06

CPC classification number: G02B21/367 ,  G02B21/12 ,  G02B21/244 ,  G02B21/245

Abstract: Some embodiments of the invention include a system comprising a positioning device configured to a hold a sample and adjust a position of a sample in response to receiving a drift compensation signal; a first light source disposed to transilluminate the sample; a second light source disposed to epi-illuminate the sample; an optical system configured to receive light from the sample and generate a three-dimensional point spread function from the light from the sample; an image sensor disposed relative to the optical system that produces an image from the light collected from the sample via the optical system; and logic electrically coupled with the image detector and the positioning device, the logic configured to determine one or more drift compensation values from images imaged by the image detector, and configured to send one or more drift compensation signals to the positioning device.

Abstract translation: 本发明的一些实施例包括一种包括定位装置的系统，该定位装置被配置为保持样本并响应于接收漂移补偿信号而调整样本的位置; 设置成透过样品的第一光源; 第二光源，被设置为表面照射样品; 光学系统，被配置为从所述样品接收光，并从所述样品的光产生三维点扩散函数; 相对于所述光学系统设置的图像传感器，其从通过所述光学系统从所述样本收集的光产生图像; 以及与图像检测器和定位装置电耦合的逻辑，该逻辑被配置为从由图像检测器成像的图像中确定一个或多个漂移补偿值，并且被配置为向定位装置发送一个或多个漂移补偿信号。