公开(公告)号：US09823194B2

公开(公告)日：2017-11-21

申请号：US14743535

申请日：2015-06-18

Applicant: Singulex, Inc.

Inventor： Philippe J. Goix ,  Robert Puskas ,  John Todd ,  Richard A. Livingston ,  Douglas Held

IPC: G01N21/64 ,  G01N33/533 ,  G01N33/543 ,  G01N33/68 ,  G01N33/58

CPC classification number: G01N21/6428 ,  G01N33/533 ,  G01N33/54306 ,  G01N33/582 ,  G01N33/68 ,  G01N2201/06113 ,  G01N2201/062

Abstract: Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.

公开(公告)号：US20140206632A1

公开(公告)日：2014-07-24

申请号：US14161420

申请日：2014-01-22

Applicant: Singulex, Inc.

Inventor： John Todd ,  Joel Estis ,  Victoria Torres

IPC: G01N33/543

CPC classification number: G01N33/6869 ,  A61B5/412 ,  C12Q1/6883 ,  G01N33/6887 ,  G01N33/6893 ,  G01N2333/475 ,  G01N2333/525 ,  G01N2333/5412 ,  G01N2333/58 ,  G01N2800/325 ,  G01N2800/50 ,  G01N2800/52

Abstract: A method for determining the risk, severity or progression of cardiovascular disease, such as cardiac heart failure. A method for determining the likelihood of admission to the hospital for cardiac heart failure. The methods include determining the concentration of ET-1 and the concentration of one or more of biomarkers selected from the group consisting of cardiac troponin (e.g., cTnI, cTnT), VEGF, BNP, NT-proBNP, and IL-6 in a blood, serum or plasma sample from the patient.

Abstract translation: 确定心血管疾病如心脏衰竭的风险，严重程度或发展的方法。 确定进入心脏心脏衰竭的可能性的方法。 所述方法包括确定ET-1的浓度以及选自血液中的心肌肌钙蛋白（例如cTnI，cTnT），VEGF，BNP，NT-proBNP和IL-6中的一种或多种生物标志物的浓度 ，来自患者的血清或血浆样品。

公开(公告)号：US20160202270A1

公开(公告)日：2016-07-14

申请号：US14921553

申请日：2015-10-23

Applicant: Singulex, Inc. ,  The Regents of the University of California

Inventor： Philippe J. Goix ,  Robert Puskas ,  John Todd ,  Richard Livingston ,  Douglas Held ,  Allan H.B. Wu

IPC: G01N33/68

CPC classification number: G01N33/68 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/582 ,  G01N33/6887 ,  G01N33/6893 ,  G01N2333/47 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/325 ,  G01N2800/50

Abstract: The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

公开(公告)号：US09063131B2

公开(公告)日：2015-06-23

申请号：US13802857

申请日：2013-03-14

Applicant: Singulex, Inc.

Inventor： Philippe J. Goix ,  Robert Puskas ,  John Todd ,  Richard A. Livingston ,  Douglas Held

IPC: C12M1/34 ,  G01N33/543 ,  G01N21/64 ,  G01N33/533 ,  G01N33/68

CPC classification number: G01N21/6428 ,  G01N33/533 ,  G01N33/54306 ,  G01N33/582 ,  G01N33/68 ,  G01N2201/06113 ,  G01N2201/062

Abstract: Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.

Abstract translation: 公开了用于分子的高灵敏度检测的方法，试剂盒和组合物。 方法，试剂盒和组合物可用于测定样品中分子的浓度为1飞摩尔，1 attomolar或更低的水平。 方法，试剂盒和组合物还允许在宽范围内测定浓度，例如7-log范围，而不需要样品稀释。

公开(公告)号：US20130261009A1

公开(公告)日：2013-10-03

申请号：US13791981

申请日：2013-03-09

Applicant: SINGULEX, INC.

Inventor： Philippe J. Goix ,  Robert Puskas ,  John Todd ,  Richard Livingston ,  Douglas Held ,  Sara Le

IPC: G01N33/68

CPC classification number: G01N33/6869 ,  G01N33/6893 ,  G01N2333/32 ,  G01N2333/47 ,  G01N2333/4712 ,  G01N2333/525 ,  G01N2333/54 ,  G01N2333/5412 ,  G01N2333/58 ,  G01N2800/32 ,  G01N2800/325 ,  G01N2800/60

Abstract: Cardiovascular disease, e.g., congestive heart failure, is often first diagnosed after the onset of clinical symptoms, eliminating potential for early intervention. The invention provides a multi-marker immunoassay, including cardiac pathology and vascular inflammation biomarkers, yielding a more sensitive assay for early detection of CHF in plasma. A panel consisting of cardiac pathology (cTnI, BNP) and vascular inflammation (IL-6, TNFα, IL-17a) biomarkers provided a sensitivity of 94% for association with CHF.

Abstract translation: 心血管疾病，例如充血性心力衰竭，经常在临床症状发作后首先诊断，消除早期干预的可能性。 本发明提供了多标记免疫测定法，包括心脏病理学和血管炎症生物标志物，产生用于血浆中CHF早期检测的更灵敏的测定。 由心脏病理学（cTnI，BNP）和血管炎症（IL-6，TNFalpha，IL-17a）生物标志物组成的小组提供了与CHF相关的94％的灵敏度。

公开(公告)号：US09977031B2

公开(公告)日：2018-05-22

申请号：US15348120

申请日：2016-11-10

Applicant: Singulex, Inc. ,  The Regents of the University of California

Inventor： Philippe Goix ,  Robert Puskas ,  John Todd ,  Richard Livingston ,  Douglas Held ,  Allan H. B. Wu

IPC: G01N33/58 ,  G01N33/68 ,  G01N33/577

CPC classification number: G01N33/6887 ,  G01N15/06 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/577 ,  G01N33/582 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2201/1247 ,  G01N2201/12761 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/52 ,  Y10T436/105831

Abstract: The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin, Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

公开(公告)号：US20170153248A1

公开(公告)日：2017-06-01

申请号：US15348120

申请日：2016-11-10

Applicant: Singulex, Inc. ,  The Regents of the University of California

Inventor： Philippe Goix ,  Robert Puskas ,  John Todd ,  Richard Livingston ,  Douglas Held ,  Allan H.B. Wu

IPC: G01N33/68 ,  G01N33/577 ,  G01N33/58

CPC classification number: G01N33/6887 ,  G01N15/06 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/577 ,  G01N33/582 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2201/1247 ,  G01N2201/12761 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/52 ,  Y10T436/105831

Abstract: The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin, Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

公开(公告)号：US09068991B2

公开(公告)日：2015-06-30

申请号：US13791981

申请日：2013-03-09

Applicant: Singulex, Inc.

Inventor： Philippe J. Goix ,  Robert Puskas ,  John Todd ,  Richard Livingston ,  Douglas Held ,  Sara Le

IPC: G01N33/53 ,  G01N33/68

CPC classification number: G01N33/6869 ,  G01N33/6893 ,  G01N2333/32 ,  G01N2333/47 ,  G01N2333/4712 ,  G01N2333/525 ,  G01N2333/54 ,  G01N2333/5412 ,  G01N2333/58 ,  G01N2800/32 ,  G01N2800/325 ,  G01N2800/60

Abstract: Cardiovascular disease, e.g., congestive heart failure, is often first diagnosed after the onset of clinical symptoms, eliminating potential for early intervention. The invention provides a multi-marker immunoassay, including cardiac pathology and vascular inflammation biomarkers, yielding a more sensitive assay for early detection of CHF in plasma. A panel consisting of cardiac pathology (cTnI, BNP) and vascular inflammation (IL-6, TNFα, IL-17a) biomarkers provided a sensitivity of 94% for association with CHF.

Abstract translation: 心血管疾病，例如充血性心力衰竭，经常在临床症状发作后首先诊断，消除早期干预的可能性。 本发明提供了多标记免疫测定法，包括心脏病理学和血管炎症生物标志物，产生用于血浆中CHF早期检测的更灵敏的测定。 由心脏病理学（cTnI，BNP）和血管炎症（IL-6，TNFα，IL-17a）生物标志物组成的小组提供了与CHF相关的94％的灵敏度。

公开(公告)号：US20130130403A1

公开(公告)日：2013-05-23

申请号：US13667372

申请日：2012-11-02

Applicant: SINGULEX, INC.

Inventor： Philippe Goix ,  Robert Puskas ,  John Todd ,  Richard Livingston ,  Douglas Held ,  Allan H.B. Wu

IPC: G01N21/64 ,  G01N33/53 ,  G01N33/566

CPC classification number: G01N33/68 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/582 ,  G01N33/6887 ,  G01N33/6893 ,  G01N2333/47 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/325 ,  G01N2800/50

Abstract: The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

Abstract translation: 本发明提供了用于敏感检测心肌肌钙蛋白的方法，组合物，试剂盒和系统。 这些方法，组合物，试剂盒和系统在涉及释放心肌肌钙蛋白的病症中的诊断，预后和测定方法中是有用的。

公开(公告)号：US20180100862A1

公开(公告)日：2018-04-12

申请号：US15660783

申请日：2017-07-26

Applicant: Singulex, Inc. ,  The Regents of the University of California

Inventor： Philippe J. Goix ,  Robert Puskas ,  John Todd ,  Richard Livingston ,  Douglas Held ,  Allan H.B. Wu

IPC: G01N33/68 ,  G01N21/64 ,  G01N33/58

CPC classification number: G01N33/68 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/582 ,  G01N33/6887 ,  G01N33/6893 ,  G01N2333/47 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/325 ,  G01N2800/50

Abstract: The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.