公开(公告)号：US20210302426A1

公开(公告)日：2021-09-30

申请号：US17264749

申请日：2019-08-05

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Yasushi OCHIAI ,  Tomofumi NAKAMURA

IPC: G01N33/569

Abstract: An object of the present invention is to provide an immunological detection method comprising carrying out an immunoreaction using an antibody to Mycoplasma pneumoniae protein P30, in which Mycoplasma pneumoniae is detected with high sensitivity. Provided is an immunological detection method for Mycoplasma pneumoniae using an antibody to Mycoplasma pneumoniae protein P30, the method comprising carrying out an immunoreaction in the presence of a polyoxyethylene alkyl amine. The immunological detection method that uses immunochromatography is also provided.

公开(公告)号：US20240255505A1

公开(公告)日：2024-08-01

申请号：US18290089

申请日：2022-06-16

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Jiro HIROTA ,  Satoru UNO ,  Yasushi OCHIAI ,  Shizuka ITO ,  Keisuke WATANABE ,  Shinya OKUYAMA ,  Tomohide ASAI

IPC: G01N33/569 ,  C07K14/005 ,  C07K16/10

CPC classification number: G01N33/56983 ,  C07K14/005 ,  C07K16/1002 ,  C07K2317/565 ,  C12N2770/20022 ,  G01N2333/165

Abstract: The present invention provides an immunoassay method for assaying SARS-CoV-2 in a biological sample, including using two types of monoclonal antibodies or antibody fragments thereof that bind to a peptide fragment having 30 or less consecutive amino acids in a nucleocapsid protein of SARS-CoV-2, wherein the two types of monoclonal antibodies or antibody fragments thereof recognize different epitopes

公开(公告)号：US20190145970A1

公开(公告)日：2019-05-16

申请号：US16098074

申请日：2017-07-12

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Kimiyoshi NISHITANI ,  Yasushi OCHIAI

IPC: G01N33/543

Abstract: A problem to be solved by the present invention is to provide a method of inhibiting nonspecific reaction unique to feces in an immunochromatographic detection method in which a fecal sample is developed in an insoluble membrane to detect an analyte in the sample. It was found that nonspecific reaction caused by a fecal sample can be inhibited to accurately detect an analyte in the fecal sample by using a compound having in the molecule two or more carboxy groups. Particularly, it was found that nonspecific reaction unable to be inhibited by conventional methods can be inhibited for fecal samples of infants by using the sample diluent containing a compound having in the molecule two or more carboxy groups for the present invention, and the present invention was thereby completed.

公开(公告)号：US20240377396A1

公开(公告)日：2024-11-14

申请号：US18574523

申请日：2022-08-09

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Shohei YAJI ,  Shizuka ITO ,  Shinya OKUYAMA ,  Yasushi OCHIAI ,  Keigo KOHNO ,  Keisuke WATANABE ,  Christopher Kenta HEWSON ,  Shun SAKAI

IPC: G01N33/569 ,  G01N33/543 ,  G01N33/58

Abstract: The present invention provides an immunoassay method for detecting a protein with an isoelectric point of 9.5 or higher, comprising a step of bringing a sample containing the protein with an isoelectric point of 9.5 or higher into contact with a glass material in the presence of a cationic substance, and an immunochromatography kit for detecting a protein with an isoelectric point of 9.5 or higher, comprising a specimen diluent and an immunochromatography test strip.

公开(公告)号：US20180088110A1

公开(公告)日：2018-03-29

申请号：US15558416

申请日：2016-08-03

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Yasushi OCHIAI ,  Kimiyoshi NISHITANI

IPC: G01N33/543 ,  G01N33/569 ,  C07K16/42

CPC classification number: G01N33/54393 ,  C07K16/42 ,  C07K16/4283 ,  G01N33/531 ,  G01N33/543 ,  G01N33/56983 ,  G01N2333/08

Abstract: A problem is to provide a method of inhibiting nonspecific reaction unique to feces in a detection method using immunochromatography developing a fecal sample in an insoluble membrane so as to detect an analyte in the sample, and an immunochromatographic test strip used therefor. The present invention provides a method of performing an immunochromatographic reaction in the presence of an anti-IgA antibody so as to detect an analyte in a fecal sample without being affected by nonspecific reaction, and an immunochromatographic test strip used therefor.

公开(公告)号：US20240241120A1

公开(公告)日：2024-07-18

申请号：US18290077

申请日：2022-06-16

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Jiro HIROTA ,  Satoru UNO ,  Yasushi OCHIAI ,  Shohei YAJI ,  Christopher Kenta HEWSON ,  Keigo KOHNO ,  Tomohide ASAI

IPC: G01N33/569 ,  C07K16/10

CPC classification number: G01N33/56983 ,  C07K16/1003 ,  C07K2317/34 ,  G01N2333/165

Abstract: The present invention provides an immunoassay method for assaying SARS-CoV-2, including a step of contacting a biological sample with a monoclonal antibody or an antibody fragment thereof that binds to a nucleocapsid protein of SARS-COV-2, wherein the monoclonal antibody or the antibody fragment thereof binds to the SARS-CoV-2 treated with a serine protease.