公开(公告)号：US07659363B2

公开(公告)日：2010-02-09

申请号：US11537790

申请日：2006-10-02

申请人： Franz-Josef Rubroeder ,  Reinhold Keller ,  Heike Herbert

发明人： Franz-Josef Rubroeder ,  Reinhold Keller ,  Heike Herbert

IPC分类号： A61K38/00

CPC分类号： C07K14/62

摘要： The present invention comprises a process for preparing insulin or an insulin derivative with correctly linked cysteine bridges from a precursor of said insulin or insulin derivative, wherein said precursor is subjected to a folding process in the presence of cysteine or cysteine hydrochloride and a chaotropic auxiliary compound. The insulin or insulin derivative with correctly linked cysteine bridges is obtained by enzymic cleavage by means of trypsin or a trypsin-like enzyme and, where appropriate, additionally by means of carboxypeptidase B and subsequent purification on an adsorber resin, which process is carried out at varied pH and temperature ranges.

摘要翻译： 本发明包括一种从所述胰岛素或胰岛素衍生物的前体制备具有正确连接的半胱氨酸桥的胰岛素或胰岛素衍生物的方法，其中所述前体在半胱氨酸或半胱氨酸盐酸盐和离液序列辅助化合物存在下进行折叠过程 。 具有正确连接的半胱氨酸桥的胰岛素或胰岛素衍生物通过利用胰蛋白酶或类胰蛋白酶进行酶切，并且在适当的情况下通过羧肽酶B进行酶切，随后在吸附树脂上纯化，该过程在 pH和温度范围不同。

公开(公告)号：US5756346A

公开(公告)日：1998-05-26

申请号：US589999

申请日：1996-01-23

申请人： Lothar Willms ,  Gerd Fulling ,  Reinhold Keller

发明人： Lothar Willms ,  Gerd Fulling ,  Reinhold Keller

IPC分类号： C07F9/30 ,  C07F9/32 ,  C12P13/02 ,  C12P13/04 ,  C12P41/00 ,  C07C231/16

CPC分类号： C12P41/00 ,  C07F9/301 ,  C07F9/3211 ,  C12P13/02 ,  C12P13/04 ,  Y10S435/874 ,  Y10S435/876

摘要： L-PTC (L-phosphinothricin, L-2-amino-4-methylphosphino-butyric acid) is the active herbicidal component of D,L-PTC and can be obtained according to the invention when D,L-PTC derivatives which are N-acylated and esterified on the phosphinic acid group as well as optionally esterified or amidated on the carboxylic group, are treated with a hydrolytically active enzyme in an aqueous or aqueous-organic medium, in which process the L-PTC derivatives are selectively hydrolyzed on the N-acyl group or the modified carboxyl group, the resulting product mixture is resolved, and the desired L-PTC derivative is hydrolyzed to give the L-PTC and isolated by customary methods.

公开(公告)号：US5380659A

公开(公告)日：1995-01-10

申请号：US924799

申请日：1992-08-06

申请人： Wolfgang Holla ,  Reinhold Keller

发明人： Wolfgang Holla ,  Reinhold Keller

IPC分类号： C07D309/30 ,  C12P17/06 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12P7/00

CPC分类号： C07D309/30 ,  C12P17/06 ,  Y10S435/921

摘要： A process for highly regioselective esterification and ester cleavage on unsaturated sugar compounds with the aid of lipases and esterases, and products which can be prepared by this process.Highly regioselective esterifications and ester cleavages can be carried out on unsaturated sugar compounds with the aid of lipases and esterases. Lipases from microorganisms or from the pancreas and liver of animals are preferably used.

摘要翻译： 借助于脂肪酶和酯酶的不饱和糖化合物的高度区域选择性酯化和酯裂解的方法，以及可以通过该方法制备的产物。 借助于脂肪酶和酯酶，可以在不饱和糖化合物上进行高度区域选择性酯化和酯裂解。 优选使用来自微生物或来自动物的胰脏和肝脏的脂肪酶。

公开(公告)号：US4709075A

公开(公告)日：1987-11-24

申请号：US805618

申请日：1985-12-06

申请人： Reinhold Keller

发明人： Reinhold Keller

IPC分类号： C07C231/00 ,  C07C67/00 ,  C07C231/02 ,  C07C233/49 ,  C07K5/075 ,  C07C67/313

CPC分类号： C07K5/0613

摘要： Alkyl N-maleylphenylalanate is prepared by reacting the starting material alkyl L-phenylalanate and maleic anhydride in aqueous solution at a pH of at most 7.5. The reaction products can be isomerized with dipolar aprotic solvents into the corresponding fumaric acid monoamides and thus can be used as an intermediate in the preparation of alkyl .alpha.-L-aspartyl-L-phenylalanate.

摘要翻译： 烷基N-马来酰基苯基丙氨酸烷基酯通过使原料烷基L-苯基丙氨酸酯和马来酸酐在至多7.5的pH的水溶液中反应来制备。 反应产物可以用偶极非质子溶剂异构化成相应的富马酸单酰胺，因此可用作制备α-L-天冬氨酰基-L-苯丙氨酸烷基酯的中间体。

公开(公告)号：US5010012A

公开(公告)日：1991-04-23

申请号：US231290

申请日：1988-08-12

申请人： Dieter Wullbrandt ,  Reinhold Keller ,  Merten Schlingmann ,  Wolfgang Holla ,  Manfred Schneider

发明人： Dieter Wullbrandt ,  Reinhold Keller ,  Merten Schlingmann ,  Wolfgang Holla ,  Manfred Schneider

IPC分类号： A01N57/20 ,  C07F9/30 ,  C07F9/32 ,  C07F9/53 ,  C12P9/00 ,  C12P41/00

CPC分类号： C12P41/005 ,  A01N57/20 ,  C07F9/301 ,  C07F9/3211 ,  C12P9/00

摘要： Resolution of the racemates of phosphorus-containing functional acetic acid derivatives can be carried out in high yield and in enantiomeric purity using hydrolases such as esterases and lipases.

摘要翻译： 含磷官能乙酸衍生物的外消旋物的拆分可以使用水解酶如酯酶和脂肪酶以高产率和对映体纯度进行。

公开(公告)号：US4859590A

公开(公告)日：1989-08-22

申请号：US80086

申请日：1987-07-31

申请人： Joachim Thiem ,  Wolfgang Treder ,  Reinhold Keller ,  Merten Schlingmann

发明人： Joachim Thiem ,  Wolfgang Treder ,  Reinhold Keller ,  Merten Schlingmann

IPC分类号： C08B37/16 ,  C12P19/18

CPC分类号： C12P19/18

摘要： .alpha.-Glucosyl fluoride can be converted in the presence of cyclodextrin .alpha.(1.fwdarw.4)glucosyltransferase into a mixture of .alpha.- and .beta.-cyclodextrins in high yield.

摘要翻译： α-葡萄糖基氟可以在环糊精α（1-> 4）葡糖基转移酶的存在下以高产率转化成α-和β-环糊精的混合物。

公开(公告)号：US4745059A

公开(公告)日：1988-05-17

申请号：US749465

申请日：1985-06-27

申请人： Hartmut Voelskow ,  Reinhold Keller ,  Merten Schlingmann ,  Martin Platen ,  Johann Then ,  Gerhard Wohner

发明人： Hartmut Voelskow ,  Reinhold Keller ,  Merten Schlingmann ,  Martin Platen ,  Johann Then ,  Gerhard Wohner

IPC分类号： C12P13/22 ,  C12R1/01 ,  C12R1/19 ,  C12R1/465 ,  C12N1/36 ,  C12N11/04

CPC分类号： C12P13/222

摘要： L-Phenylalanine can be prepared using microorganisms belonging to the series comprising E. coli, Paracoccus denitrificans, Torula, Rhodotorula or Streptomyces after adaptation to phenylpyruvic acid, by amination with suitable sources of nitrogen. It is advantageous to employ the microorganism in the form of fixed cells.

摘要翻译： 可以通过用合适的氮源胺化，使用属于包含大肠杆菌，反硝化副球菌，Torula，Rhodotorula或Streptomyces的系列的微生物在适应苯丙酮酸之后制备L-苯丙氨酸。 使用固定细胞形式的微生物是有利的。

公开(公告)号：US4649111A

公开(公告)日：1987-03-10

申请号：US764967

申请日：1985-08-12

申请人： Reinhold Keller ,  Merten Schlingmann

发明人： Reinhold Keller ,  Merten Schlingmann

IPC分类号： C12P19/32 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C12N11/08 ,  C12N11/12 ,  C12P19/30 ,  C12N11/02

CPC分类号： C07H19/16 ,  C07H19/20 ,  C12N11/08 ,  C12N11/12 ,  C12P19/30

摘要： Ribonucleic acid in a mixture of crude nucleic acids is selectively hydrolyzed with 5'-phosphodiesterase to 5'-ribonucleotides without hydrolyzing desoxyribonucleic acid in the mixture. Selective hydrolysis is carrying out by contacting the crude nucleic acid mixture with 5'-phosphodiesterase immobilized on a polymer carrier. The crude mixture of nucleic acids is preferably obtained by aqueous extraction of microorganisms that have previously been extracted with ammonia and a lower alcohol to remove lipids. The polymer carrier is preferably a copolymer of glycidylmethacrylate, allylglycidylether, methacrylamide and methylene-bis-methacrylamide.

摘要翻译： 将粗核酸混合物中的核糖核酸与5'-磷酸二酯酶选择性水解成5'-核糖核苷酸，而不会水解脱氧核糖核酸在混合物中。 通过使粗核酸混合物与固定在聚合物载体上的5'-磷酸二酯酶接触来进行选择性水解。 核酸的粗混合物优选通过水提取先前用氨和低级醇萃取以除去脂质的微生物获得。 聚合物载体优选为甲基丙烯酸缩水甘油酯，烯丙基缩水甘油醚，甲基丙烯酰胺和亚甲基双 - 甲基丙烯酰胺的共聚物。

公开(公告)号：US4576973A

公开(公告)日：1986-03-18

申请号：US623708

申请日：1984-06-22

申请人： Karl-Heinz Keil ,  Fritz Engelhardt ,  Ulrich Greiner ,  Klaus Kuhlein ,  Reinhold Keller ,  Merten Schlingmann ,  Gerhard Hess

发明人： Karl-Heinz Keil ,  Fritz Engelhardt ,  Ulrich Greiner ,  Klaus Kuhlein ,  Reinhold Keller ,  Merten Schlingmann ,  Gerhard Hess

IPC分类号： C08F26/00 ,  B01J20/26 ,  B01J41/14 ,  C08F2/18 ,  C08F30/00 ,  C08F30/04 ,  C08F30/08 ,  C08F226/06 ,  C08J9/00

CPC分类号： C08F226/06 ,  B01J20/26

摘要： The crosslinked, porous copolymer in the form of beads and containing 50 to 99.9% by weight of recurring units of a heterocyclic 5-membered ring compound which contains a polymerizable olefinic group and which has at least one protonizable nitrogen atom in the ring, 0.1 to 50% by weight of units of a crosslinking agent, 0 to 25% by weight of units of a polymerizable organoboron or organosilicon compound and, relative to 100% by weight of the sum of the abovementioned units, 5 to 350% by weight of units of an N-vinylamide of the general formula I CH.sub.2 .dbd.CH--N(R.sup.1)--C(R.sup.2).dbd.O wherein R.sup.1 denotes hydrogen, methyl or ethyl and R.sup.2 denotes hydrogen or C.sub.1 -C.sub.3 -alkyl, or several such N-vinylamides and 0 to 40% by weight of units of another polymerizable compound or several such compounds, is prepared by inverted suspension polymerization and is used, for example, as a sorbent for acid substances from solutions thereof, preferably aqueous solutions.

摘要翻译： 珠粒形式的交联的多孔共聚物，其含有50-99.9重量％的含有可聚合烯基的杂环5元环化合物的重复单元，该环化合物在环中具有至少一个可质子化的氮原子，0.1至 50重量％的交联剂单元，0〜25重量％的可聚合有机硼或有机硅化合物的单元，相对于100重量％的上述单元的总和，为5〜350重量％的单元 的通式I CH 2 = CH-N（R 1）-C（R 2）= O的N-乙烯基酰胺，其中R 1表示氢，甲基或乙基，R 2表示氢或C 1 -C 3烷基，或几个这样的N-乙烯基酰胺 和0〜40重量％的其它可聚合化合物或几种这样的化合物的单元通过反相悬浮聚合制备，并且例如用作其溶液，优选水溶液中的酸性物质的吸附剂。

公开(公告)号：US06401582B1

公开(公告)日：2002-06-11

申请号：US09468599

申请日：1999-12-21

申请人： Rolf Noé ,  Andreas Noé ,  Reinhold Keller

发明人： Rolf Noé ,  Andreas Noé ,  Reinhold Keller

IPC分类号： B26D714

CPC分类号： B65B69/0025 ,  Y10S83/924 ,  Y10T83/343 ,  Y10T83/538 ,  Y10T83/75 ,  Y10T83/7573 ,  Y10T83/8746

摘要： An apparatus for removing binder straps from coils of metal strip in which a cage is engageable with the coil and has a blade carriage displaceable over at least a region in which binder straps may be provided in a continuous manner so that a wedge can engage beneath the straps in succession and a cutting tool can cut through straps in a single continuous operation without interrupting the movement of the carriage. The wedge and the carriage can be provided with clamping elements to engage the strap after it is cut to allow it to be transported to a scrap bin.

摘要翻译： 一种用于从金属带的线圈移除粘结带的装置，其中保持架可与线圈接合，并且具有可移动到至少一个可以以连续方式设置粘合带的区域的刀架，使得楔可以接合在 连续的带子和切割工具可以在单个连续操作中切割带子而不中断托架的移动。 楔形件和托架可以设置有夹紧元件，以在其被切割之后接合带子，以允许其被运送到废料仓。