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    ASSAY DEVICE AND METHOD
    1.
    发明申请
    ASSAY DEVICE AND METHOD 审中-公开

    公开(公告)号：US20190072552A1

    公开(公告)日：2019-03-07

    申请号：US16136451

    申请日：2018-09-20

    Applicant: President and Fellows of Harvard College

    Inventor： Samuel K. Sia ,  Vincent Linder ,  Babak Parviz ,  Adam Carlyn Siegel ,  George M. Whitesides

    IPC: G01N33/569 ,  G01N33/543

    Abstract: An assay device and method are described. The assay may involve immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion of a surface of a microfluidic chamber; passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.

    ASSAY DEVICE AND METHOD
    6.
    发明申请
    ASSAY DEVICE AND METHOD 审中-公开
    测定装置和方法

    公开(公告)号：US20140134603A1

    公开(公告)日：2014-05-15

    申请号：US14042606

    申请日：2013-09-30

    Applicant: President and Fellows of Harvard College

    Inventor： Samuel K. Sia ,  Vincent Linder ,  Babak Amir-parviz ,  Adam Siegel ,  George M. Whitesides

    IPC: G01N33/569 ,  G01N33/543

    CPC classification number: G01N33/56988 ,  B01L3/502707 ,  B01L2300/0822 ,  B01L2300/168 ,  G01N21/05 ,  G01N21/59 ,  G01N33/5302 ,  G01N33/543 ,  G01N33/585 ,  G01N2021/0346 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339

    Abstract: An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion of a surface of a microfluidic chamber; passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.

    Abstract translation: 描述了一种测定方法，其包括将待检测分析物（例如抗体或抗原）的结合配偶体（例如，抗原或抗体）固定在微流体室表面的一部分上的步骤; 将流体样品通过表面并允许分析物结合到结合配偶体; 允许金属胶体，例如金共轭抗体与结合的分析物缔合; 在表面上流动金属溶液，例如银溶液，以形成不透明的金属层; 并且例如通过目视检查或通过在将金属溶液流过表面之后测量金属溶液中的层的透光率，层的导电性或电阻或金属浓度来检测所述金属层的存在。

    FLUIDIC STRUCTURES INCLUDING MEANDERING AND WIDE CHANNELS
    7.
    发明申请
    FLUIDIC STRUCTURES INCLUDING MEANDERING AND WIDE CHANNELS 有权
    流体结构包括测量和宽频道

    公开(公告)号：US20130330748A1

    公开(公告)日：2013-12-12

    申请号：US13860160

    申请日：2013-04-10

    Applicant: President and Fellows of Harvard College ,  OPKO Diagnostics, LLC

    Inventor： Vincent Linder ,  Samuel K. Sia ,  George M. Whitesides ,  Max Narovlyansky ,  Adam Siegel

    IPC: G01N33/543

    CPC classification number: G01N33/54366 ,  B01L3/502746 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0883 ,  B01L2400/0406 ,  G01N21/01 ,  G01N21/82 ,  G01N33/54373

    Abstract: The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including meandering and wide channels. Microfluidic systems can provide an advantageous environment for performing various reactions and analyses due to a reduction in sample and reagent quantities that are required, a reduction in the size of the operating system, and a decrease in reaction time compared to conventional systems. Unfortunately, the small size of microfluidic channels can sometimes result in difficulty in detecting a species without magnifying optics (such as a microscope or a photomultiplier). A series of tightly packed microchannels, i.e., a meandering region, or a wide channel having a dimension on the order of millimeters, can serve as a solution to this problem by creating a wide measurement area. Although this invention mainly describes the use of meandering and wide channels in heterogeneous immunoassays on a microfluidic chip, this invention could be used for amplifying optical signals for other types of reactions and/or assays.

    Abstract translation: 本发明一般涉及微流体结构，更具体地涉及包括曲折和宽通道的微流体结构和方法。 微流控系统可以提供用于执行各种反应和分析的有利环境，这是由于与常规系统相比所需的样品和试剂量的减少，操作系统尺寸的减小以及反应时间的减少。 不幸的是，微量流体通道的小尺寸有时会导致在不放大光学器件（例如显微镜或光电倍增管）的情况下检测物质的困难。 一系列紧密包装的微通道，即曲折区域或具有大约毫米数量级的宽通道可以通过创建宽的测量区域来解决这个问题。 尽管本发明主要描述了在微流体芯片上的异源免疫测定中使用曲折和宽通道，但是本发明可用于放大用于其他类型反应和/或测定的光学信号。

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