公开(公告)号：USRE46992E1

公开(公告)日：2018-08-14

申请号：US15284238

申请日：2016-10-03

Applicant: PREMIUM GENETICS (UK) LTD.

Inventor： Daniel Mueth ,  Amy L. Morjal ,  Christopher R. Knutson ,  Joseph Plewa

IPC: G01N21/47 ,  G01N21/01 ,  G01N21/64 ,  G01N15/14 ,  C12M1/00

CPC classification number: G01N21/6428 ,  C12M47/04 ,  G01N15/1404 ,  G01N15/1459 ,  G01N2015/1409 ,  G01N2015/1411 ,  G01N2015/149

Abstract: Apparatus for sorting and orienting sperm cells has a pair or walls in confronting relationship forming a flow chamber having inlet, a downstream outlet, and intermediate detector region. The inlet receives first and second spaced apart streams of input fluid and a third stream of sample fluid containing the cells to be sorted. The first and second streams have respective flow rates relative to third stream, such that the third stream is constricted forming a relatively narrow sample stream, so that the cells are oriented parallel to the walls. A detector detects desired cells and a sorter downstream of the detector for sorting the desired cells from the stream.

公开(公告)号：US20170204370A1

公开(公告)日：2017-07-20

申请号：US15476509

申请日：2017-03-31

Applicant: Premium Genetics (UK) LTD.

Inventor： Amy L. Morjal ,  Christopher R. Knutson ,  Daniel Mueth ,  Joseph Plewa ,  Evan Tanner

IPC: C12N5/071 ,  A61D19/04 ,  C12N5/076 ,  G01N15/14 ,  G01N1/30

CPC classification number: C12N5/0612 ,  A61D19/04 ,  B82Y5/00 ,  B82Y10/00 ,  C12N5/061 ,  G01N1/30 ,  G01N15/14 ,  G01N15/1425 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/689 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149 ,  G01N2021/6439

Abstract: The present invention provides novel methods of cell staining, such as bovine sperm, using electroporation or osmolality treatments at viability-enhancing temperatures. Furthermore, methods of highly efficient cell sorting that are especially suitable in sorting bovine sperm using novel cell staining procedures are also provided.

公开(公告)号：US20170016879A1

公开(公告)日：2017-01-19

申请号：US15276747

申请日：2016-09-26

Applicant: Premium Genetics (UK) LTD.

Inventor： Amy L. Morjal ,  Christopher R. Knutson ,  Daniel Mueth ,  Joseph Plewa ,  Evan Tanner

IPC: G01N33/50 ,  G01N21/64 ,  G01N15/14

CPC classification number: C12N5/0612 ,  A61D19/04 ,  B82Y5/00 ,  B82Y10/00 ,  C12N5/061 ,  G01N1/30 ,  G01N15/14 ,  G01N15/1425 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/689 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149 ,  G01N2021/6439

Abstract: The present invention provides novel methods of cell staining, such as bovine sperm, using electroporation or osmolality treatments at viability-enhancing temperatures. Furthermore, methods of highly efficient cell sorting that are especially suitable in sorting bovine sperm using novel cell staining procedures are also provided.

Abstract translation: 本发明提供新的细胞染色方法，例如牛精子，在活力增强温度下使用电穿孔或渗透压浓度处理。 此外，还提供了使用新型细胞染色方法特别适用于分选牛精子的高效细胞分选方法。