公开(公告)号：US20170204370A1

公开(公告)日：2017-07-20

申请号：US15476509

申请日：2017-03-31

Applicant: Premium Genetics (UK) LTD.

Inventor： Amy L. Morjal ,  Christopher R. Knutson ,  Daniel Mueth ,  Joseph Plewa ,  Evan Tanner

IPC: C12N5/071 ,  A61D19/04 ,  C12N5/076 ,  G01N15/14 ,  G01N1/30

CPC classification number: C12N5/0612 ,  A61D19/04 ,  B82Y5/00 ,  B82Y10/00 ,  C12N5/061 ,  G01N1/30 ,  G01N15/14 ,  G01N15/1425 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/689 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149 ,  G01N2021/6439

Abstract: The present invention provides novel methods of cell staining, such as bovine sperm, using electroporation or osmolality treatments at viability-enhancing temperatures. Furthermore, methods of highly efficient cell sorting that are especially suitable in sorting bovine sperm using novel cell staining procedures are also provided.

公开(公告)号：US20170016879A1

公开(公告)日：2017-01-19

申请号：US15276747

申请日：2016-09-26

Applicant: Premium Genetics (UK) LTD.

Inventor： Amy L. Morjal ,  Christopher R. Knutson ,  Daniel Mueth ,  Joseph Plewa ,  Evan Tanner

IPC: G01N33/50 ,  G01N21/64 ,  G01N15/14

CPC classification number: C12N5/0612 ,  A61D19/04 ,  B82Y5/00 ,  B82Y10/00 ,  C12N5/061 ,  G01N1/30 ,  G01N15/14 ,  G01N15/1425 ,  G01N21/6428 ,  G01N33/5005 ,  G01N33/689 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149 ,  G01N2021/6439

Abstract: The present invention provides novel methods of cell staining, such as bovine sperm, using electroporation or osmolality treatments at viability-enhancing temperatures. Furthermore, methods of highly efficient cell sorting that are especially suitable in sorting bovine sperm using novel cell staining procedures are also provided.

Abstract translation: 本发明提供新的细胞染色方法，例如牛精子，在活力增强温度下使用电穿孔或渗透压浓度处理。 此外，还提供了使用新型细胞染色方法特别适用于分选牛精子的高效细胞分选方法。