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公开(公告)号:US20190185901A1
公开(公告)日:2019-06-20
申请号:US16222862
申请日:2018-12-17
Applicant: OLYMPUS CORPORATION
Inventor: Katsunori OGO
Abstract: Provided is a sample processing method that liquefies a medium solution by making a liquid that liquefies the medium solution act on a sample formed by gelating or solidifying the medium solution that is supported by a substrate while an observation subject is included therein, while maintaining a state in which the medium solution is supported by the substrate while the observation subject is included therein.
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2.
公开(公告)号:US20190184400A1
公开(公告)日:2019-06-20
申请号:US16222739
申请日:2018-12-17
Applicant: OLYMPUS CORPORATION
Inventor: Katsunori OGO
CPC classification number: B01L3/56 , B01L2200/0647 , B01L2200/16 , G01N1/30
Abstract: Provided is a microscope-observation-sample preparation base material including: a base material body that includes: a flow path in which a medium solution is made to flow; a solution injection section that opens at one end of the flow path and into which the medium solution is injected; and a droplet supporting section that opens at the other end of the flow path and that supports a droplet of the medium solution injected from the solution injection section, in a hanging state, with a surface of the droplet being partially exposed, and an outside-air entry preventing member that prevents entry of outside air from the solution injection section into the droplet supporting section, in the flow path of the base material body.
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公开(公告)号:US20210373312A1
公开(公告)日:2021-12-02
申请号:US17327891
申请日:2021-05-24
Applicant: OLYMPUS CORPORATION
Inventor: Yoshihiro SHIMADA , Chihiro HIRAGA , Katsunori OGO , Daisuke NISHIWAKI , Takashi SUGIYAMA
Abstract: An observation vessel includes a holding portion configured to hold an observation object and an accommodation portion at least partially formed of a transparent curved surface and configured to position the holding portion. The holding portion is configured to hold the observation object at a set position separated from the curved surface toward a center of curvature of the curved surface in a first state of being positioned at a first position by the accommodation portion.
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公开(公告)号:US20150291938A1
公开(公告)日:2015-10-15
申请号:US14250866
申请日:2014-04-11
Applicant: OLYMPUS CORPORATION , PERAK STATE DEVELOPMENT CORPORATION , NIMURA GENETIC SOLUTIONS CO., LTD.
Inventor: Ryutaro AKIYOSHI , Katsunori OGO , Hirobumi SUZUKI
CPC classification number: C12N9/0069 , C12Q1/66 , C12Y113/12007
Abstract: The present invention relates to a luciferase derived from a Malaysian Luciola firefly, the luciferase having a maximum luminescent wavelength of 557 nm at pH 8, or the luciferase inducing luminescence having 1.5 times or more the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.
Abstract translation: 本发明涉及来自马来西亚卢西亚萤火虫的荧光素酶,荧光素酶在pH8时具有最大发光波长为557nm,或荧光素酶诱导具有1.5倍以上由光alis萤火虫荧光素酶诱导的发光的发光强度的荧光素酶。
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公开(公告)号:US20140073031A1
公开(公告)日:2014-03-13
申请号:US14025950
申请日:2013-09-13
Applicant: OLYMPUS CORPORATION , PERAK STATE DEVELOPMENT CORPORATION , NIMURA GENETIC SOLUTIONS CO., LTD.
Inventor: Ryutaro AKIYOSHI , Katsunori OGO , Hirobumi SUZUKI
IPC: C12N9/02
CPC classification number: C12N9/0069
Abstract: The present invention relates to a luciferase derived from a star-worm belonging to genus Diplocladon, the luciferase inducing luminescence such that a maximum luminous wavelength falls within a range of 557 to 562 nm over the entire pH range of 5.5 to 8.0, or the luciferase inducing luminescence having 1.5 times the luminous intensity of luminescence induced by Photinus pyralis firefly luciferase.
Abstract translation: 本发明涉及源自属于Diplocladon属的星虫的荧光素酶,荧光素酶诱导发光,使得最大发光波长在5.5〜8.0的整个pH范围内落入557〜562nm的范围内,或荧光素酶 诱导具有由Photinus pyralis萤火虫萤光素酶诱导的发光发光强度的1.5倍的发光。
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公开(公告)号:US20190136180A1
公开(公告)日:2019-05-09
申请号:US16176976
申请日:2018-10-31
Applicant: OLYMPUS CORPORATION
Inventor: Katsunori OGO
Abstract: Provided is a method for preparing a sample for microscope examination, the method including bringing a liquid (B) for gelling or solidifying a medium solution (A) into contact with a surface of the medium solution (A), which contains at least one spheroid (S) and which is substantially transparent when being gelled or solidified, in a state where the liquid (B) is in the form of mist, bubbles, or liquid droplets with a smaller volume than the volume of the medium solution (A), thereby gelling or solidifying the medium solution (A).
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公开(公告)号:US20170204379A1
公开(公告)日:2017-07-20
申请号:US15472919
申请日:2017-03-29
Applicant: OLYMPUS CORPORATION
Inventor: Katsunori OGO
IPC: C12N9/02
CPC classification number: C12N9/0069 , C12Y113/12007
Abstract: A luciferase according to an embodiment comprises an amino acid sequence in which a mutation is introduced into an amino acid sequence of SEQ ID NO: 38. The luciferase catalyzes a luminescence reaction that generates luminescence having the maximum luminescent wavelength of 570 nm to 610 nm. The luminescence has an intensity at least 10 times higher than that of luminescence generated in a luminescence reaction catalyzed by a luciferase having an amino acid sequence of SEQ ID NO: 48.
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8.
公开(公告)号:US20140080199A1
公开(公告)日:2014-03-20
申请号:US14023673
申请日:2013-09-11
Applicant: OLYMPUS CORPORATION
Inventor: Katsunori OGO , Ryutaro AKIYOSHI , Mariko MURAI , Takashi KINEBUCHI , Hirobumi SUZUKI
IPC: C12N9/02
CPC classification number: C12N9/0069 , C07K14/43563
Abstract: An object of the invention is to provide a novel and useful luciferase. The luciferase according to the embodiments of the invention is derived from Lucidina accensa.
Abstract translation: 本发明的目的是提供一种新颖且有用的荧光素酶。 根据本发明的实施方案的荧光素酶得自Lucidina accensa。
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