SPLIT RECOMBINANT LUCIFERASE, AND ANALYSIS METHOD USING THEREOF

    公开(公告)号:US20170183637A1

    公开(公告)日:2017-06-29

    申请号:US15451939

    申请日:2017-03-07

    Inventor: Takashi SUGIYAMA

    CPC classification number: C12N9/0069 C12N15/52 C12N15/62 C12Q1/26 C12Q1/66

    Abstract: Disclosed is a split recombinant protein that includes N-terminal and C-terminal fragments of a firefly luciferase, and a linker peptide. The N-terminal fragment is one of two fragments of a firefly luciferase split into two at a splitting position specific to the firefly luciferase. The C-terminal fragment includes a C-terminal fragment of a firefly luciferase split into two at a splitting position specific to the firefly luciferase, and 58 to 78 amino acid residues toward the N-terminal beyond the splitting position. When the N-terminal and C-terminal fragments are bound together, firefly luciferase activity is exhibited.

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