公开(公告)号：US20190049709A1

公开(公告)日：2019-02-14

申请号：US16050171

申请日：2018-07-31

Applicant: OLYMPUS CORPORATION

Inventor： Yoshihiro SHIMADA ,  Go RYU

IPC: G02B21/06 ,  F21V8/00 ,  G02B21/16

Abstract: A light sheet microscope includes a light source configured to output light, a plurality of optical fibers configured to guide the light that is from the light source, a plurality of illumination optical systems configured to respectively emit beams of the light guided from the plurality of optical fibers toward a sample as light sheets in a plurality of different directions on a same plane, and a light switching device that is provided between the light source and the plurality of optical fibers and that is configured to switch an optical fiber that the light output from the light source enters, from among the plurality of optical fibers.

公开(公告)号：US20170371138A1

公开(公告)日：2017-12-28

申请号：US15622072

申请日：2017-06-13

Applicant: OLYMPUS CORPORATION

Inventor： Go RYU

IPC: G02B21/00 ,  G02B21/36 ,  G02B21/08

CPC classification number: G02B21/0076 ,  G02B21/082 ,  G02B21/16 ,  G02B21/365

Abstract: Provided is a microscope including: a detection optical system detecting fluorescence produced in a specimen to acquire fluorescence images; an illumination device causing planar excitation light to be incident on the specimen from different directions along a plurality of incident planes that are parallel to each other with a prescribed spacing therebetween in the direction along the detection optical axis of the system; a drive portion causing relative movement between: the system and the device; and the specimen, in the direction along the detection optical axis, in a state in which each of the incident planes and the focal plane of the system are aligned for each sheet of excitation light; and an image processing unit combining fluorescence images that are acquired when respective sheets of excitation light are incident from the different directions along the same incident plane at different times while the portion causes the relative movement.

公开(公告)号：US20160320601A1

公开(公告)日：2016-11-03

申请号：US15131613

申请日：2016-04-18

Applicant: OLYMPUS CORPORATION

Inventor： Go RYU

IPC: G02B21/16 ,  H04N5/225 ,  G02B21/06 ,  G02B21/36 ,  G02B21/26 ,  G02B21/24

CPC classification number: G02B21/16 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/06 ,  G02B21/241 ,  G02B21/26 ,  G02B21/367 ,  H04N5/2256

Abstract: In order to acquire clear images when different positions on a specimen in the direction of the optical axis of an illumination optical system are observed with a sheet illumination method, a microscope apparatus of the present invention includes: a detection optical system that detects fluorescence produced in a specimen immersed in a medium having a different refractive index from air and accommodated in a container and that acquires a fluorescence image; an illumination device that focuses excitation light emitted from an excitation-light source, in the form of a plane along a plane intersecting an optical axis of the detection optical system and that makes the planar excitation light incident on the specimen in the container; a driving unit that moves the specimen in the direction of the optical axis of a cylindrical lens; and an illumination control unit that adjusts the focal position of the illumination device on the basis of a movement amount of the specimen moved by the driving unit such that the air-equivalent length along the optical path of the excitation light from the illumination device to the optical axis of the detection optical system is maintained constant.

Abstract translation: 本发明的显微镜装置包括：检测光学系统，其检测在照射光学系统的光轴方向上的不同位置的照明光学系统的光轴上的不同位置， 浸渍在具有与空气不同的折射率的介质中的试样，容纳在容器中并获取荧光图像; 照明装置，其以与所述检测光学系统的光轴相交的平面的方式对从激励光源发出的激励光进行聚焦，并使所述平面激发光入射到所述容器内的样本上; 驱动单元，其沿着柱面透镜的光轴的方向移动所述试样; 以及照明控制单元，其基于由所述驱动单元移动的样本的移动量，使得沿着从所述照明装置到所述照明装置的激发光的光路的空气当量长度来调整所述照明装置的焦点位置 检测光学系统的光轴保持恒定。

公开(公告)号：US20160124203A1

公开(公告)日：2016-05-05

申请号：US14922757

申请日：2015-10-26

Applicant: OLYMPUS CORPORATION

Inventor： Go RYU

IPC: G02B21/00 ,  G06T7/00 ,  G02B21/36

CPC classification number: G02B21/367 ,  G02B21/0048 ,  G02B21/06 ,  G02B21/16 ,  G02B26/105

Abstract: To prevent unwanted exposure of a specimen with excitation light despite magnification switching, a microscope of the present invention includes: a detection optical system that detects fluorescence emitted from the specimen; a light-sheet illumination optical system, serving as an excitation light source, that causes planar excitation light to be incident along a direction intersecting an optical axis of the detection optical system; and a control section that, when the magnification of the detection optical system is raised, controls the light-sheet illumination optical system so as to reduce a radiation width of the excitation light made incident on the specimen by the light-sheet illumination optical system.

Abstract translation: 为了尽可能地进行放大切换来防止用激发光照射不必要的试样，本发明的显微镜包括：检测从样本发出的荧光的检测光学系统; 用作激发光源的光板照明光学系统，其使平面激发光沿着与检测光学系统的光轴相交的方向入射; 以及控制部，当检测光学系统的倍率升高时，控制光照照明光学系统，以便减少由光照照明光学系统入射到样本上的激发光的辐射宽度。

公开(公告)号：US20190041622A1

公开(公告)日：2019-02-07

申请号：US16038668

申请日：2018-07-18

Applicant: OLYMPUS CORPORATION

Inventor： Go RYU

IPC: G02B21/16 ,  G02B21/36 ,  H04N5/225

Abstract: A light sheet illumination microscope includes: an illumination optical system that irradiates a medium with sheet-shaped excitation light; a detection optical system that has an optical axis that intersects an optical axis of the illumination optical system, and detects fluorescence emitted by the medium that is irradiated with the light; a focus adjustment mechanism that changes a focus position of the detection optical system in a direction of the optical axis; and a controller. The controller controls, according to at least a first refractive index and a second refractive index, the focus adjustment mechanism when a wavelength of the fluorescence detected by the detection optical system is switched from a first wavelength to a second wavelength, the first refractive index being a refractive index of the medium with respect to the first wavelength, the second refractive index being a refractive index of the medium with respect to the second wavelength.

公开(公告)号：US20190025564A1

公开(公告)日：2019-01-24

申请号：US16035989

申请日：2018-07-16

Applicant: OLYMPUS CORPORATION

Inventor： Go RYU

IPC: G02B21/06 ,  G02B21/00

Abstract: A light sheet illumination microscope 100 includes: an illumination optical system 15 that emits excitation light to a sample from a plurality of directions; an up-and-down stage 11 and a tilt stage 12 that respectively change an emission position and an emission direction of the excitation light emitted by the illumination optical system 15; and a controller 20 that controls the up-and-down stage 11 and the tilt stage 12 according to a switching of a wavelength of the excitation light emitted by the illumination optical system 15, so as to correct a deviation between irradiated planes formed by pieces of excitation light emitted from the plurality of directions.