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    CULTURE MEDIUM COMPOSITION
    1.
    发明申请
    CULTURE MEDIUM COMPOSITION 审中-公开
    文化中成分

    公开(公告)号：US20170009201A1

    公开(公告)日：2017-01-12

    申请号：US15113762

    申请日：2015-01-23

    申请人： NISSAN CHEMICAL INDUSTRIES, LTD. ,  KYOTO UNIVERSITY

    发明人： Hisato HAYASHI ,  Misayo OTANI ,  Koichiro SARUHASHI ,  Taito NISHINO ,  Takehisa IWAMA ,  Tatsuro KANAKI ,  Ayako AIHARA

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0068 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/78 ,  C12N2535/00

    摘要： The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition having an effect of preventing sedimentation of cells and/or tissues, which is afforded by substantially retaining the cells and/or tissues without substantially increasing the viscosity of the solution by nanofibers which have been added to the solution and uniformly dispersed in the liquid medium, and the like.

    摘要翻译： 本发明通过使用具有抑制细胞和/或组织沉淀的作用的培养基组合来提供包含培养细胞和/或悬浮状态的组织的细胞和/或组织的培养方法，所述培养基组合物通过基本上保持细胞 和/或组织，而基本上不会增加​​已经加入到溶液中并均匀分散在液体介质中的纳米纤维的溶液粘度等。

    METHOD FOR PRODUCING CULTURE MEDIUM COMPOSITION
    5.
    发明申请
    METHOD FOR PRODUCING CULTURE MEDIUM COMPOSITION 审中-公开
    生产培养基组合物的方法

    公开(公告)号：US20170002311A1

    公开(公告)日：2017-01-05

    申请号：US15113753

    申请日：2015-01-23

    申请人： NISSAN CHEMICAL INDUSTRIES, LTD. ,  KYOTO UNIVERSITY

    发明人： Misayo OTANI ,  Koichiro SARUHASHI ,  Taito NISHINO ,  Ayako AIHARA ,  Hisashi ISHII ,  Keigo MATSUMOTO

    IPC分类号： C12N5/00 ,  C08B37/00

    CPC分类号： C12N5/0018 ,  C08B37/006 ,  C12N5/0025 ,  C12N2500/34

    摘要： The present invention provides a production method of a medium composition, including previously removing divalent metal cations contained in a polymer compound having an anionic functional group, preparing a dry powder of the polymer compound, and mixing the dry powder and the medium, and the like. The present invention also provides a purification method of a polymer compound having an anionic functional group, which includes mixing a suspension of the polymer compound having an anionic functional group in water and a cation exchanger that exchanges a divalent metal cation with a monovalent metal cation to give a polymer compound having an anionic functional group, which shows a reduced amount of contaminating divalent metal cations.

    摘要翻译： 本发明提供了一种介质组合物的制备方法，其包括预先除去具有阴离子官能团的高分子化合物中所含的二价金属阳离子，制备高分子化合物的干燥粉末，以及混合干燥粉末和介质等 。 本发明还提供了具有阴离子官能团的高分子化合物的纯化方法，该方法包括将水中具有阴离子官能团的高分子化合物的悬浮液和将二价金属阳离子与一价金属阳离子交换的阳离子交换剂混合 得到具有阴离子官能团的高分子化合物，其显示出减少量的污染二价金属阳离子。

    METHOD FOR PREPARING LIQUID MEDIUM COMPOSITION, AND PREPARATION DEVICE AND KIT THEREFOR
    9.
    发明申请
    METHOD FOR PREPARING LIQUID MEDIUM COMPOSITION, AND PREPARATION DEVICE AND KIT THEREFOR 有权

    公开(公告)号：US20220267480A1

    公开(公告)日：2022-08-25

    申请号：US17742130

    申请日：2022-05-11

    申请人： NISSAN CHEMICAL INDUSTRIES, LTD.

    发明人： Hisato HAYASHI ,  Koichiro SARUHASHI ,  Tatsuro KANAKI

    IPC分类号： C08B37/00 ,  C12N5/00 ,  A61J1/20 ,  B01J19/24 ,  C08J3/205

    摘要： The present invention provides a method capable of easily mixing any liquid containing a linking substance such as a divalent metal cation and the like with a liquid containing a particular compound at a high concentration, and capable of producing a liquid medium composition comprising fine structures dispersed therein, and a production device therefor and a kit therefor. The first liquid containing a particular compound is passed through a through-hole having a given cross-sectional area formed in a nozzle part at a given flow rate and injected into the second liquid at a given flow rate. By this simple operation, a structure in which the particular compound is bonded via the linking substance is formed, and the structure is preferably dispersed in a mixture of the both liquids.