TISSUE FRAGMENT
    7.
    发明申请

    公开(公告)号:US20210130757A1

    公开(公告)日:2021-05-06

    申请号:US17097987

    申请日:2020-11-13

    申请人: KYOTO UNIVERSITY

    摘要: Provided is a method for producing a tissue-like construct having cultured cells organized in an aligned manner, especially, a cardiac tissue-like construct. The tissue-like construct prepared by this method is also provided. Further provided are a device for evaluating cellular electrophysiological functions and a method for evaluating cellular electrophysiological functions. Furthermore, a sheet-like cell culture scaffold is also provided.

    METHOD FOR SUBCULTURING PLURIPOTENT STEM CELLS

    公开(公告)号:US20190078058A1

    公开(公告)日:2019-03-14

    申请号:US16180005

    申请日:2018-11-05

    IPC分类号: C12N5/074

    摘要: The present disclosure provides a method for subculturing pluripotent stem cells suitable for mass culture. The method for subculturing pluripotent stem cells includes: a culture step of culturing pluripotent stem cells to obtain a cell aggregation; and a dividing step of dividing the cell aggregation by passing the cell aggregation through a mesh-like film, which has a plurality of through-holes each having an opening dimension of 30 μm to 80 μm, at a speed of 15 cm/sec to 150 cm/sec.

    Frozen Storage Container and Frozen Storage Container System

    公开(公告)号:US20190159968A1

    公开(公告)日:2019-05-30

    申请号:US16313410

    申请日:2017-06-27

    摘要: There is provided means with which, during frozen storage of a biological sample or a drug, the biological sample or drug stored inside a frozen storage container is not susceptible to contamination.A frozen storage container 10 includes a container body 11 with an internal space 13, and a partition wall 12 which divides the internal space 13 of the container body 11 at least into a first space 14 and a second space 15. In the container body 11, the first space 14 is liquid-tightly sealed, the second space 15 has an opening 22 which is in communication with the outside, and the second space 15 is liquid-tightly sealable by welding the container body 11. A frozen storage container system 40 includes the frozen storage container 10; and a needle member 41 which includes a needle tip portion 42 capable of being inserted into the internal space 13 of the container body 11 and capable of piercing the partition wall 12, and has a flow path which is opened at the needle tip portion 42 and is opened at a base end portion 43 opposite to the needle tip portion 42.