公开(公告)号：US20210130757A1

公开(公告)日：2021-05-06

申请号：US17097987

申请日：2020-11-13

申请人： KYOTO UNIVERSITY

发明人： Li LIU ,  Junjun LI ,  Itsunari MINAMI ,  Yong CHEN ,  Norio NAKATSUJI

IPC分类号： C12M3/00 ,  C12M1/12 ,  A61K35/12 ,  C12M1/34 ,  A61F2/24

摘要： Provided is a method for producing a tissue-like construct having cultured cells organized in an aligned manner, especially, a cardiac tissue-like construct. The tissue-like construct prepared by this method is also provided. Further provided are a device for evaluating cellular electrophysiological functions and a method for evaluating cellular electrophysiological functions. Furthermore, a sheet-like cell culture scaffold is also provided.

公开(公告)号：US20200325448A1

公开(公告)日：2020-10-15

申请号：US16084132

申请日：2017-03-17

申请人： KYOTO UNIVERSITY

发明人： Itsunari MINAMI

IPC分类号： C12N5/077 ,  A01N1/02 ,  A61K35/34 ,  G01N33/50

摘要： The disclosure provides a method of freezing an aggregate of pluripotent stem cell-derived cardiomyocytes, comprising: (i) immersing an aggregate of pluripotent stem cell-derived cardiomyocytes in a cryoprotective solution; and (ii) freezing the aggregate immersed in the cryoprotective solution. The disclosure also provides a frozen aggregate of pluripotent stem cell-derived cardiomyocytes frozen by the method.