公开(公告)号：US20090251155A1

公开(公告)日：2009-10-08

申请号：US12288971

申请日：2008-10-24

申请人： Lei Wang ,  Jing Zhu ,  Cheng Deng ,  Jing Cheng

发明人： Lei Wang ,  Jing Zhu ,  Cheng Deng ,  Jing Cheng

IPC分类号： G01R27/00

CPC分类号： G01N33/5029 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/16 ,  G01N33/5438

摘要： Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal.

摘要翻译： 公开了用于检测阻抗的技术，系统和装置。 一方面，微电极感测装置包括基板和形成在基板上的微电极传感器阵列。 每个传感器包括形成在衬底上的至少一个导电层，并被图案化以包括对电极和多个感测电极，以在不存在和存在位于每个感测的表面的至少一部分上的一个或多个靶单元的情况下检测电信号 电极。 感测电极间隔开并布置在对电极周围，以提供检测到的电信号的空间平均值。

公开(公告)号：US08779779B2

公开(公告)日：2014-07-15

申请号：US12288971

申请日：2008-10-24

申请人： Lei Wang ,  Jing Zhu ,  Cheng Deng ,  Jing Cheng

发明人： Lei Wang ,  Jing Zhu ,  Cheng Deng ,  Jing Cheng

IPC分类号： G01R27/28

CPC分类号： G01N33/5029 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/16 ,  G01N33/5438

摘要： Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal.

摘要翻译： 公开了用于检测阻抗的技术，系统和装置。 一方面，微电极感测装置包括基板和形成在基板上的微电极传感器阵列。 每个传感器包括形成在衬底上的至少一个导电层，并被图案化以包括对电极和多个感测电极，以在不存在和存在位于每个感测的表面的至少一部分上的一个或多个靶单元的情况下检测电信号 电极。 感测电极间隔开并布置在对电极周围，以提供检测到的电信号的空间平均值。

公开(公告)号：US20090000948A1

公开(公告)日：2009-01-01

申请号：US12097409

申请日：2007-01-04

申请人： Lei Wang ,  Lei Wang ,  Min Guo ,  Jing Cheng

发明人： Lei Wang ,  Lei Wang ,  Min Guo ,  Jing Cheng

IPC分类号： G01N27/26 ,  G01N27/00

CPC分类号： C12N13/00 ,  C12M23/16 ,  C12M33/00 ,  C12M35/02

摘要： The present invention provides methods for enhancing the efficiency of cell electroporation using dielectrophoresis-assisted cell localization and uses thereof in a microfluidic biochip system. Cells are first subject to dielectrophoresis and localized to regions where the electric field intensity is high enough to render cells electroporated. The invention enhances the efficiency of in situ cell electroporation on a traditional microfluidic biochip.

摘要翻译： 本发明提供使用介电电泳辅助细胞定位提高细胞电穿孔效率的方法及其在微流体生物芯片系统中的用途。 细胞首先进行介导电泳，并定位于电场强度足以使细胞电穿孔的区域。 本发明增强了传统微流体生物芯片上原位细胞电穿孔的效率。

公开(公告)号：US08784633B2

公开(公告)日：2014-07-22

申请号：US12667507

申请日：2007-07-04

申请人： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

发明人： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N33/4836 ,  B01J2219/00653 ,  B01J2219/00743 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2400/0421 ,  B01L2400/0424

摘要： A microelectrode sensing device includes a substrate and an array of microelectrode sensors. Each sensor includes a first conductive layer that at least partially conducts electricity. The first conductive layer is formed above the substrate and patterned to include a recording electrode that measures electrical activities of target cells. Each sensor also includes a second conductive layer that at least partially conducts electricity. The second conductive layer is elevated above the first layer and patterned to include multiple positioning electrodes arranged to define a sensing region above the recording electrode. The positioning electrodes are designed to generate an electric field pattern in the sensing region to move and confine the target cells to a sub-region of the sensing region that at least partially overlaps the recording electrode.

摘要翻译： 微电极感测装置包括基板和微电极传感器阵列。 每个传感器包括至少部分地导电的第一导电层。 第一导电层形成在衬底上并被图案化以包括测量靶细胞的电活动的记录电极。 每个传感器还包括至少部分地传导电的第二导电层。 第二导电层在第一层之上被升高并且被图案化以包括布置成限定记录电极上方的感测区域的多个定位电极。 定位电极被设计成在感测区域中产生电场图案以移动并将目标单元限制在至少部分地与记录电极重叠的感测区域的子区域上。

公开(公告)号：US20090322309A1

公开(公告)日：2009-12-31

申请号：US12424955

申请日：2009-04-16

申请人： Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

发明人： Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

IPC分类号： G01N27/00 ,  H05K1/16

CPC分类号： G01N33/4836

摘要： Among others things, techniques, systems, and apparatus are disclosed for recording electrophysiological signals. In one aspect, a microelectrode sensing device includes a printed circuit board (PCB), a chip unit electrically connected to the PCB, and a cell culture chamber positioned over the chip unit and sealed to the PCB with the chip unit between the PCB and the cell culture chamber. The chip unit includes a substrate; a conductive layer positioned over the substrate that includes one or more recording electrodes; an insulation layer positioned over the conductive layer; another conductive layer positioned over the insulation layer that includes positioning electrodes; and another insulation layer positioned over the other conductive layer. The recording and positioning electrodes are electrically independent so as to independently receive a stimulus signal at each recording electrode and positioning electrode and independently detect a sensed signal at each recording electrode.

摘要翻译： 公开了用于记录电生理信号的技术，系统和装置。 在一个方面，微电极感测装置包括印刷电路板（PCB），与PCB电连接的芯片单元和位于芯片单元上方并且密封到PCB的细胞培养室，芯片单元位于PCB和PCB之间 细胞培养室。 芯片单元包括基板; 位于所述基板上方的包括一个或多个记录电极的导电层; 位于导电层上的绝缘层; 位于包括定位电极的绝缘层之上的另一个导电层; 以及位于另一导电层上的另一绝缘层。 记录和定位电极是电独立的，以便在每个记录电极和定位电极处独立地接收刺激信号，并独立地检测每个记录电极处的感测信号。

公开(公告)号：US08901913B2

公开(公告)日：2014-12-02

申请号：US12424955

申请日：2009-04-16

申请人： Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

发明人： Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

IPC分类号： G01N27/00 ,  G01N33/483

CPC分类号： G01N33/4836

摘要： Among others things, techniques, systems, and apparatus are disclosed for recording electrophysiological signals. In one aspect, a microelectrode sensing device includes a printed circuit board (PCB), a chip unit electrically connected to the PCB, and a cell culture chamber positioned over the chip unit and sealed to the PCB with the chip unit between the PCB and the cell culture chamber. The chip unit includes a substrate; a conductive layer positioned over the substrate that includes one or more recording electrodes; an insulation layer positioned over the conductive layer; another conductive layer positioned over the insulation layer that includes positioning electrodes; and another insulation layer positioned over the other conductive layer. The recording and positioning electrodes are electrically independent so as to independently receive a stimulus signal at each recording electrode and positioning electrode and independently detect a sensed signal at each recording electrode.

摘要翻译： 公开了用于记录电生理信号的技术，系统和装置。 在一个方面，微电极感测装置包括印刷电路板（PCB），与PCB电连接的芯片单元和位于芯片单元上方并且密封到PCB的细胞培养室，芯片单元位于PCB和PCB之间 细胞培养室。 芯片单元包括基板; 位于所述基板上方的包括一个或多个记录电极的导电层; 位于导电层上的绝缘层; 位于包括定位电极的绝缘层之上的另一个导电层; 以及位于另一导电层上的另一绝缘层。 记录和定位电极是电独立的，以便在每个记录电极和定位电极处独立地接收刺激信号，并独立地检测每个记录电极处的感测信号。

公开(公告)号：US20100270176A1

公开(公告)日：2010-10-28

申请号：US12667507

申请日：2007-07-04

申请人： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

发明人： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

IPC分类号： G01N33/50 ,  G01N27/26

CPC分类号： G01N33/4836 ,  B01J2219/00653 ,  B01J2219/00743 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2400/0421 ,  B01L2400/0424

摘要： A microelectrode sensing device includes a substrate and an array of microelectrode sensors. Each sensor includes a first conductive layer that at least partially conducts electricity. The first conductive layer is formed above the substrate and patterned to include a recording electrode that measures electrical activities of target cells. Each sensor also includes a second conductive layer that at least partially conducts electricity. The second conductive layer is elevated above the first layer and patterned to include multiple positioning electrodes arranged to define a sensing region above the recording electrode. The positioning electrodes are designed to generate an electric field pattern in the sensing region to move and confine the target cells to a sub-region of the sensing region that at least partially overlaps the recording electrode.

摘要翻译： 微电极感测装置包括基板和微电极传感器阵列。 每个传感器包括至少部分地导电的第一导电层。 第一导电层形成在衬底上并被图案化以包括测量靶细胞的电活动的记录电极。 每个传感器还包括至少部分地传导电的第二导电层。 第二导电层在第一层之上被升高并且被图案化以包括布置成限定记录电极上方的感测区域的多个定位电极。 定位电极被设计成在感测区域中产生电场图案以移动并将目标单元限制在至少部分地与记录电极重叠的感测区域的子区域上。

公开(公告)号：US10365213B2

公开(公告)日：2019-07-30

申请号：US12997223

申请日：2008-06-11

申请人： Jiang Zhu ,  Cheng Deng ,  Guoliang Huang ,  Shukuan Xu ,  Jing Cheng

发明人： Jiang Zhu ,  Cheng Deng ,  Guoliang Huang ,  Shukuan Xu ,  Jing Cheng

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N21/27 ,  G01N21/64

摘要： Methods and devices use in two-color measurement systems. The methods and devices include methods of making corrections, methods of calculating correction factors, fluorescence scanners, and microarray chips. The said methods and devices enable a user to correct fluorescence intensities for errors caused by the occurrence of FRET and/or cross-talk when two fluorophores are used in two-color fluorescence arrays.

公开(公告)号：US20110301062A1

公开(公告)日：2011-12-08

申请号：US12997223

申请日：2008-06-11

申请人： Jiang Zhu ,  Cheng Deng ,  Guoliang Huang ,  Shukuan Xu ,  Jing Cheng

发明人： Jiang Zhu ,  Cheng Deng ,  Guoliang Huang ,  Shukuan Xu ,  Jing Cheng

IPC分类号： C40B30/10 ,  C40B50/14 ,  C40B60/12 ,  C40B60/14

CPC分类号： G01N21/278 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6441

摘要： Methods and devices use in two-color measurement systems. The methods and devices include methods of making corrections, methods of calculating correction factors, fluorescence scanners, and microarray chips. The said methods and devices enable a user to correct fluorescence intensities for errors caused by the occurrence of FRET and /or cross-talk when two fluorophores are used in two-color fluorescence arrays.

摘要翻译： 方法和设备用于双色测量系统。 方法和装置包括进行校正的方法，计算校正因子的方法，荧光扫描仪和微阵列芯片。 所述方法和装置使得用户能够在双色荧光阵列中使用两个荧光团时，校正由FRET和/或串扰的发生引起的误差的荧光强度。

公开(公告)号：US09511366B2

公开(公告)日：2016-12-06

申请号：US13991848

申请日：2012-01-13

申请人： Rui Ma ,  Lan Xie ,  Tian Qiu ,  Lei Wang ,  Wanli Xing ,  Jing Cheng

发明人： Rui Ma ,  Lan Xie ,  Tian Qiu ,  Lei Wang ,  Wanli Xing ,  Jing Cheng

IPC分类号： B01L3/00 ,  C12M3/00 ,  C12M1/32 ,  C12M3/06

CPC分类号： B01L3/5027 ,  C12M21/06 ,  C12M23/12 ,  C12M23/16

摘要： A micro fluidic device comprises one microstructure layer (5) and one cover layer (1), wherein the cover layer (1) is connected to the microstructure layer (5). The microstructure layer (5) comprises one bottom layer and a plurality of microstructures on it to position samples. The cover layer (1) comprises one top layer, one positioning well (6) and at least one inlet pool (4). The positioning well (6) is right above the microstructures and connected with each other. The inlet pools (4) and the positioning well (6) are connected by microchannels (3) which are formed between the microstructure layer (5) and the cover layer (1). The micro fluidic device can be applied in vitro fertilization, in determining how glial cells affect neurons, in constructing neural network and in detecting cell growth conditions.

摘要翻译： 微流体装置包括一个微结构层（5）和一个覆盖层（1），其中覆盖层（1）连接到微结构层（5）。 微结构层（5）包括一个底层和多个微结构，用于定位样品。 覆盖层（1）包括一个顶层，一个定位孔（6）和至少一个入口池（4）。 定位井（6）位于微结构的正上方并相互连接。 入口池（4）和定位井（6）通过形成在微结构层（5）和覆盖层（1）之间的微通道（3）连接。 微流体装置可以应用于体外受精，确定神经胶质细胞如何影响神经元，构建神经网络和检测细胞生长条件。