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公开(公告)号：US20090251155A1
公开(公告)日：2009-10-08
申请号：US12288971
申请日：2008-10-24
申请人： Lei Wang , Jing Zhu , Cheng Deng , Jing Cheng
发明人： Lei Wang , Jing Zhu , Cheng Deng , Jing Cheng
IPC分类号： G01R27/00
CPC分类号： G01N33/5029 , B01L3/502761 , B01L2200/10 , B01L2200/12 , B01L2300/0636 , B01L2300/0645 , B01L2300/069 , B01L2300/0816 , B01L2300/16 , G01N33/5438
摘要： Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal.
摘要翻译： 公开了用于检测阻抗的技术，系统和装置。 一方面，微电极感测装置包括基板和形成在基板上的微电极传感器阵列。 每个传感器包括形成在衬底上的至少一个导电层，并被图案化以包括对电极和多个感测电极，以在不存在和存在位于每个感测的表面的至少一部分上的一个或多个靶单元的情况下检测电信号 电极。 感测电极间隔开并布置在对电极周围，以提供检测到的电信号的空间平均值。
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公开(公告)号：US08779779B2
公开(公告)日：2014-07-15
申请号：US12288971
申请日：2008-10-24
申请人： Lei Wang , Jing Zhu , Cheng Deng , Jing Cheng
发明人： Lei Wang , Jing Zhu , Cheng Deng , Jing Cheng
IPC分类号： G01R27/28
CPC分类号： G01N33/5029 , B01L3/502761 , B01L2200/10 , B01L2200/12 , B01L2300/0636 , B01L2300/0645 , B01L2300/069 , B01L2300/0816 , B01L2300/16 , G01N33/5438
摘要： Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal.
摘要翻译： 公开了用于检测阻抗的技术，系统和装置。 一方面，微电极感测装置包括基板和形成在基板上的微电极传感器阵列。 每个传感器包括形成在衬底上的至少一个导电层，并被图案化以包括对电极和多个感测电极，以在不存在和存在位于每个感测的表面的至少一部分上的一个或多个靶单元的情况下检测电信号 电极。 感测电极间隔开并布置在对电极周围，以提供检测到的电信号的空间平均值。
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3.发明申请公开(公告)号：US20090000948A1
公开(公告)日：2009-01-01
申请号：US12097409
申请日：2007-01-04
申请人： Lei Wang , Lei Wang , Min Guo , Jing Cheng
发明人： Lei Wang , Lei Wang , Min Guo , Jing Cheng
摘要： The present invention provides methods for enhancing the efficiency of cell electroporation using dielectrophoresis-assisted cell localization and uses thereof in a microfluidic biochip system. Cells are first subject to dielectrophoresis and localized to regions where the electric field intensity is high enough to render cells electroporated. The invention enhances the efficiency of in situ cell electroporation on a traditional microfluidic biochip.
摘要翻译： 本发明提供使用介电电泳辅助细胞定位提高细胞电穿孔效率的方法及其在微流体生物芯片系统中的用途。 细胞首先进行介导电泳，并定位于电场强度足以使细胞电穿孔的区域。 本发明增强了传统微流体生物芯片上原位细胞电穿孔的效率。
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4.发明授权公开(公告)号：US09511366B2
公开(公告)日：2016-12-06
申请号：US13991848
申请日：2012-01-13
申请人： Rui Ma , Lan Xie , Tian Qiu , Lei Wang , Wanli Xing , Jing Cheng
发明人： Rui Ma , Lan Xie , Tian Qiu , Lei Wang , Wanli Xing , Jing Cheng
CPC分类号： B01L3/5027 , C12M21/06 , C12M23/12 , C12M23/16
摘要： A micro fluidic device comprises one microstructure layer (5) and one cover layer (1), wherein the cover layer (1) is connected to the microstructure layer (5). The microstructure layer (5) comprises one bottom layer and a plurality of microstructures on it to position samples. The cover layer (1) comprises one top layer, one positioning well (6) and at least one inlet pool (4). The positioning well (6) is right above the microstructures and connected with each other. The inlet pools (4) and the positioning well (6) are connected by microchannels (3) which are formed between the microstructure layer (5) and the cover layer (1). The micro fluidic device can be applied in vitro fertilization, in determining how glial cells affect neurons, in constructing neural network and in detecting cell growth conditions.
摘要翻译： 微流体装置包括一个微结构层（5）和一个覆盖层（1），其中覆盖层（1）连接到微结构层（5）。 微结构层（5）包括一个底层和多个微结构，用于定位样品。 覆盖层（1）包括一个顶层，一个定位孔（6）和至少一个入口池（4）。 定位井（6）位于微结构的正上方并相互连接。 入口池（4）和定位井（6）通过形成在微结构层（5）和覆盖层（1）之间的微通道（3）连接。 微流体装置可以应用于体外受精，确定神经胶质细胞如何影响神经元，构建神经网络和检测细胞生长条件。
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公开(公告)号：US08642287B2
公开(公告)日：2014-02-04
申请号：US12532992
申请日：2007-07-25
申请人： Lei Wang , Keith Mitchelson , He Wang , Jing Cheng
发明人： Lei Wang , Keith Mitchelson , He Wang , Jing Cheng
CPC分类号： G01N27/06 , G01N27/221 , G01N33/48707 , G01N33/48728 , G01N33/5438
摘要： Methods and apparatus for designing and measuring a cell-electrode impedance sensor to detect chemical and biological samples, including biological cells. The method of designing a cell-electrode impedance sensor comprises: determining a cell free cell-electrode impedance and a cell-covered cell-electrode impedance; obtaining a sensor sensitivity of the cell-electrode impedance measurement system; and choosing one or more design parameters of the cell-electrode impedance sensor to maximize the sensor sensitivity. When the frequency of AC signal between electrodes ranges from 10 kHz to 40 kHz, the sensitivity of the sensor is maximized.
摘要翻译： 用于设计和测量细胞电极阻抗传感器以检测包括生物细胞在内的化学和生物样品的方法和装置。 设计电池电极阻抗传感器的方法包括：确定无电池的电池 - 电极阻抗和电池覆盖的电池 - 电极阻抗; 获得电池电极阻抗测量系统的传感器灵敏度; 并选择电池电极阻抗传感器的一个或多个设计参数以最大化传感器灵敏度。 当电极间交流信号的频率范围为10 kHz至40 kHz时，传感器的灵敏度最大化。
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公开(公告)号：US20240009411A1
公开(公告)日：2024-01-11
申请号：US18470632
申请日：2023-09-20
申请人： Lei Wang
发明人： Lei Wang
IPC分类号： A61M15/08
CPC分类号： A61M15/08
摘要： A slow release apparatus for slowly releasing a compound in a nasal passage. The apparatus comprises a connection mechanism, two expansion mechanisms, and two release mechanisms; two ends of the connection mechanism are respectively integrally or detachably connected to the two expansion mechanisms that are symmetrically arranged in nasal passages at two sides; and the two release mechanisms are symmetrically arranged, and each release mechanism is correspondingly provided in one of the expansion mechanisms. The slow release apparatus is convenient to carry, and capable of slowly releasing a compound in the nasal passage and synchronously expanding the nasal passage to ensure smooth breathing, while considering functionality, practicability and comfort.
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公开(公告)号：USD985256S1
公开(公告)日：2023-05-09
申请号：US29852235
申请日：2022-09-06
申请人： Lei Wang
设计人： Lei Wang
摘要： FIG. 1 is a first perspective view of a walker showing my new design;
FIG. 2 is a second perspective view thereof;
FIG. 3 is a front view thereof;
FIG. 4 is a back view thereof;
FIG. 5 is a left side view thereof;
FIG. 6 is a right side view thereof;
FIG. 7 is a top view thereof; and,
FIG. 8 is a bottom view thereof.
The broken lines shown in the drawings depict portions of the walker that form no part of the claimed design.-
公开(公告)号：US11365608B2
公开(公告)日：2022-06-21
申请号：US16148684
申请日：2018-10-01
申请人： Haining Zheng , Lei Wang , Michael W. Walker
发明人： Haining Zheng , Lei Wang , Michael W. Walker
摘要： A method of moving a string assembly within a wellbore is disclosed. In some embodiments, the method comprises moving the string assembly within the wellbore; obtaining surface data regarding at least one parameter associated with moving the string assembly within the wellbore over a range of depths; modeling the at least one parameter over the range of depths for a plurality of assumed friction factors to obtain modeled data for each assumed friction factor; calculating a derivative of the surface data over the range of depths; calculating a derivative of the modeled data over the range of depths; comparing the derivative of the surface data to the derivative of the modeled data; determining one or more local friction factors for the range of depths based on the comparison; and adjusting at least one string assembly operating parameter based on the one or more local friction factors.
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公开(公告)号：US10352099B2
公开(公告)日：2019-07-16
申请号：US15208704
申请日：2016-07-13
申请人： Benjamin Spivey , Gregory S. Payette , Darren Pais , Krishnan Kumaran , Lei Wang , Jeffrey R. Bailey , Paul E. Pastusek
发明人： Benjamin Spivey , Gregory S. Payette , Darren Pais , Krishnan Kumaran , Lei Wang , Jeffrey R. Bailey , Paul E. Pastusek
摘要： Methods for drilling a wellbore within a subsurface region and drilling assemblies and systems that include and/or utilize the methods are disclosed herein. The methods include receiving a plurality of drilling performance indicator maps, normalizing the plurality of drilling performance indicator maps to generate a plurality of normalized maps, adaptive trending of the plurality of drilling performance indicator maps to generate a plurality of trended maps, summing the plurality of trended maps to generate an objective map, selecting a desired operating regime from the objective map, and adjusting at least one drilling operational parameter of a drilling rig based, at least in part, on the desired operating regime.
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公开(公告)号：US20190178059A1
公开(公告)日：2019-06-13
申请号：US16148684
申请日：2018-10-01
申请人： Haining Zheng , Lei Wang , Michael W. Walker
发明人： Haining Zheng , Lei Wang , Michael W. Walker
摘要： A method of moving a string assembly within a wellbore is disclosed. In some embodiments, the method comprises moving the string assembly within the wellbore; obtaining surface data regarding at least one parameter associated with moving the string assembly within the wellbore over a range of depths; modeling the at least one parameter over the range of depths for a plurality of assumed friction factors to obtain modeled data for each assumed friction factor; calculating a derivative of the surface data over the range of depths; calculating a derivative of the modeled data over the range of depths; comparing the derivative of the surface data to the derivative of the modeled data; determining one or more local friction factors for the range of depths based on the comparison; and adjusting at least one string assembly operating parameter based on the one or more local friction factors.
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