公开(公告)号：US09803236B2

公开(公告)日：2017-10-31

申请号：US13814213

申请日：2010-08-06

申请人： Guanbin Zhang ,  Guangxin Xiang ,  Wanli Xing ,  Jing Cheng

发明人： Guanbin Zhang ,  Guangxin Xiang ,  Wanli Xing ,  Jing Cheng

IPC分类号： C12Q1/68 ,  C40B20/02

CPC分类号： C12Q1/6837 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/155

摘要： A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises coupling a target molecule to a particle and then binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. Coupled with microarray, the particles, themselves or further modified, facilitate the detection of results with non-expensive devices or even naked eyes. This technology enables the detection and interpretation of molecular interactions in an efficient and cost effective way.

公开(公告)号：US20130217584A1

公开(公告)日：2013-08-22

申请号：US13814213

申请日：2010-08-06

申请人： Guanbin Zhang ,  Guangxin Xiang ,  Wanli Xing ,  Jing Cheng

发明人： Guanbin Zhang ,  Guangxin Xiang ,  Wanli Xing ,  Jing Cheng

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/155

摘要： A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises coupling a target molecule to a particle and then binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. Coupled with microarray, the particles, themselves or further modified, facilitate the detection of results with non-expensive devices or even naked eyes. This technology enables the detection and interpretation of molecular interactions in an efficient and cost effective way.

摘要翻译： 提供基于微阵列的测定法，其用于分析分子相互作用，包括多核苷酸，多肽，抗体，小分子化合物，肽和碳水化合物。 这种方法包括将靶分子偶联到颗粒上，然后与微阵列上的探针分子结合。 特别地，可以实现核酸片段的多重遗传分析。 可以鉴定特异性基因，单核苷酸多态性或基因突变，例如缺失，插入和插入。 与微阵列结合，颗粒本身或进一步修饰，有助于使用非昂贵装置甚至肉眼检测结果。 该技术能够以有效和成本有效的方式检测和解释分子相互作用。

公开(公告)号：US20050106708A1

公开(公告)日：2005-05-19

申请号：US10499098

申请日：2002-11-29

申请人： Wanli Xing ,  Zhe Yu ,  Guangxin Xiang ,  Liangbin Pan ,  Jing Cheng

发明人： Wanli Xing ,  Zhe Yu ,  Guangxin Xiang ,  Liangbin Pan ,  Jing Cheng

IPC分类号： C12M1/42 ,  C12M1/34 ,  C12N13/00 ,  C12Q1/02 ,  G01N33/543 ,  G01F1/64

CPC分类号： G01N33/5438 ,  C12M35/02 ,  G01N2333/46 ,  G01N2500/10

摘要： The present invention provides an apparatus for stimulating an animal cell and recording its physiological signal and methods of making and using thereof. The purpose of the present invention is to provide an apparatus for stimulating an animal cell and recording its physiological signal that is efficient, convenient, and accurate. The apparatus for stimulating an animal cell and recording its physiological signal of the present invention comprises a poor conductive substrate, wherein on at least one surface of the substrate is provided at least one unit conductive polymer layer and at least one good conductive microelectrode.

摘要翻译： 本发明提供一种用于刺激动物细胞并记录其生理信号的装置及其制造和使用方法。 本发明的目的是提供一种用于刺激动物细胞并记录其生理信号的装置，其有效，方便和准确。 用于刺激动物细胞并记录其本发明的生理信号的装置包括差的导电基底，其中在基底的至少一个表面上提供至少一个单位导电聚合物层和至少一个良好的导电微电极。

公开(公告)号：US09310375B2

公开(公告)日：2016-04-12

申请号：US13877320

申请日：2010-10-27

申请人： Huafang Gao ,  Guangxin Xiang ,  Di Jiang ,  Wanli Xing ,  Jing Cheng

发明人： Huafang Gao ,  Guangxin Xiang ,  Di Jiang ,  Wanli Xing ,  Jing Cheng

IPC分类号： G01N33/58 ,  C12Q1/68 ,  C40B30/04 ,  G01N33/543

CPC分类号： G01N33/582 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C40B30/04 ,  G01N33/54333 ,  G01N33/54386 ,  C12Q2563/103 ,  C12Q2565/501

摘要： A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises labeling a target molecule with a luminophore, coupling the target molecule to a particle, and binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. This technology, with high sensitivity, enables the detection and interpretation of molecular interactions in an efficient way.

摘要翻译： 提供基于微阵列的测定法，其用于分析分子相互作用，包括多核苷酸，多肽，抗体，小分子化合物，肽和碳水化合物。 这种方法包括用发光体标记目标分子，将靶分子偶联到颗粒上，并在微阵列上结合探针分子。 特别地，可以实现核酸片段的多重遗传分析。 可以鉴定特异性基因，单核苷酸多态性或基因突变，例如缺失，插入和插入。 该技术具有高灵敏度，能够以有效的方式检测和解释分子相互作用。

公开(公告)号：US20090322309A1

公开(公告)日：2009-12-31

申请号：US12424955

申请日：2009-04-16

申请人： Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

发明人： Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

IPC分类号： G01N27/00 ,  H05K1/16

CPC分类号： G01N33/4836

摘要： Among others things, techniques, systems, and apparatus are disclosed for recording electrophysiological signals. In one aspect, a microelectrode sensing device includes a printed circuit board (PCB), a chip unit electrically connected to the PCB, and a cell culture chamber positioned over the chip unit and sealed to the PCB with the chip unit between the PCB and the cell culture chamber. The chip unit includes a substrate; a conductive layer positioned over the substrate that includes one or more recording electrodes; an insulation layer positioned over the conductive layer; another conductive layer positioned over the insulation layer that includes positioning electrodes; and another insulation layer positioned over the other conductive layer. The recording and positioning electrodes are electrically independent so as to independently receive a stimulus signal at each recording electrode and positioning electrode and independently detect a sensed signal at each recording electrode.

摘要翻译： 公开了用于记录电生理信号的技术，系统和装置。 在一个方面，微电极感测装置包括印刷电路板（PCB），与PCB电连接的芯片单元和位于芯片单元上方并且密封到PCB的细胞培养室，芯片单元位于PCB和PCB之间 细胞培养室。 芯片单元包括基板; 位于所述基板上方的包括一个或多个记录电极的导电层; 位于导电层上的绝缘层; 位于包括定位电极的绝缘层之上的另一个导电层; 以及位于另一导电层上的另一绝缘层。 记录和定位电极是电独立的，以便在每个记录电极和定位电极处独立地接收刺激信号，并独立地检测每个记录电极处的感测信号。

公开(公告)号：US08901913B2

公开(公告)日：2014-12-02

申请号：US12424955

申请日：2009-04-16

申请人： Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

发明人： Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

IPC分类号： G01N27/00 ,  G01N33/483

CPC分类号： G01N33/4836

摘要： Among others things, techniques, systems, and apparatus are disclosed for recording electrophysiological signals. In one aspect, a microelectrode sensing device includes a printed circuit board (PCB), a chip unit electrically connected to the PCB, and a cell culture chamber positioned over the chip unit and sealed to the PCB with the chip unit between the PCB and the cell culture chamber. The chip unit includes a substrate; a conductive layer positioned over the substrate that includes one or more recording electrodes; an insulation layer positioned over the conductive layer; another conductive layer positioned over the insulation layer that includes positioning electrodes; and another insulation layer positioned over the other conductive layer. The recording and positioning electrodes are electrically independent so as to independently receive a stimulus signal at each recording electrode and positioning electrode and independently detect a sensed signal at each recording electrode.

摘要翻译： 公开了用于记录电生理信号的技术，系统和装置。 在一个方面，微电极感测装置包括印刷电路板（PCB），与PCB电连接的芯片单元和位于芯片单元上方并且密封到PCB的细胞培养室，芯片单元位于PCB和PCB之间 细胞培养室。 芯片单元包括基板; 位于所述基板上方的包括一个或多个记录电极的导电层; 位于导电层上的绝缘层; 位于包括定位电极的绝缘层之上的另一个导电层; 以及位于另一导电层上的另一绝缘层。 记录和定位电极是电独立的，以便在每个记录电极和定位电极处独立地接收刺激信号，并独立地检测每个记录电极处的感测信号。

公开(公告)号：US20130267436A1

公开(公告)日：2013-10-10

申请号：US13877320

申请日：2010-10-27

申请人： Huafang Gao ,  Guangxin Xiang ,  Di Jiang ,  Wanli Xing ,  Jing Cheng

发明人： Huafang Gao ,  Guangxin Xiang ,  Di Jiang ,  Wanli Xing ,  Jing Cheng

IPC分类号： G01N33/58 ,  C12Q1/68

CPC分类号： G01N33/582 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C40B30/04 ,  G01N33/54333 ,  G01N33/54386 ,  C12Q2563/103 ,  C12Q2565/501

摘要： A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises labeling a target molecule with a luminophore, coupling the target molecule to a particle, and binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. This technology, with high sensitivity, enables the detection and interpretation of molecular interactions in an efficient way.

摘要翻译： 提供基于微阵列的测定法，其用于分析分子相互作用，包括多核苷酸，多肽，抗体，小分子化合物，肽和碳水化合物。 这种方法包括用发光体标记目标分子，将靶分子偶联到颗粒上，并在微阵列上结合探针分子。 特别地，可以实现核酸片段的多重遗传分析。 可以鉴定特异性基因，单核苷酸多态性或基因突变，例如缺失，插入和插入。 该技术具有高灵敏度，能够以有效的方式检测和解释分子相互作用。

公开(公告)号：US08784633B2

公开(公告)日：2014-07-22

申请号：US12667507

申请日：2007-07-04

申请人： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

发明人： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N33/4836 ,  B01J2219/00653 ,  B01J2219/00743 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2400/0421 ,  B01L2400/0424

摘要： A microelectrode sensing device includes a substrate and an array of microelectrode sensors. Each sensor includes a first conductive layer that at least partially conducts electricity. The first conductive layer is formed above the substrate and patterned to include a recording electrode that measures electrical activities of target cells. Each sensor also includes a second conductive layer that at least partially conducts electricity. The second conductive layer is elevated above the first layer and patterned to include multiple positioning electrodes arranged to define a sensing region above the recording electrode. The positioning electrodes are designed to generate an electric field pattern in the sensing region to move and confine the target cells to a sub-region of the sensing region that at least partially overlaps the recording electrode.

摘要翻译： 微电极感测装置包括基板和微电极传感器阵列。 每个传感器包括至少部分地导电的第一导电层。 第一导电层形成在衬底上并被图案化以包括测量靶细胞的电活动的记录电极。 每个传感器还包括至少部分地传导电的第二导电层。 第二导电层在第一层之上被升高并且被图案化以包括布置成限定记录电极上方的感测区域的多个定位电极。 定位电极被设计成在感测区域中产生电场图案以移动并将目标单元限制在至少部分地与记录电极重叠的感测区域的子区域上。

公开(公告)号：US07632674B2

公开(公告)日：2009-12-15

申请号：US10499098

申请日：2002-11-29

申请人： Wanli Xing ,  Zhe Yu ,  Guangxin Xiang ,  Liangbin Pan ,  Jing Cheng

发明人： Wanli Xing ,  Zhe Yu ,  Guangxin Xiang ,  Liangbin Pan ,  Jing Cheng

IPC分类号： C12M3/00

CPC分类号： G01N33/5438 ,  C12M35/02 ,  G01N2333/46 ,  G01N2500/10

摘要： The present invention provides an apparatus for stimulating an animal cell and recording its physiological signal and methods of making and using thereof. The purpose of the present invention is to provide an apparatus for stimulating an animal cell and recording its physiological signal that is efficient, convenient, and accurate. The apparatus for stimulating an animal cell and recording its physiological signal of the present invention comprises a poor conductive substrate, wherein on at least one surface of the substrate is provided at least one unit conductive polymer layer and at least one good conductive microelectrode.

摘要翻译： 本发明提供一种用于刺激动物细胞并记录其生理信号的装置及其制造和使用方法。 本发明的目的是提供一种用于刺激动物细胞并记录其生理信号的装置，其有效，方便和准确。 用于刺激动物细胞并记录其本发明的生理信号的装置包括差的导电基底，其中在基底的至少一个表面上提供至少一个单位导电聚合物层和至少一个良好的导电微电极。

公开(公告)号：US20100270176A1

公开(公告)日：2010-10-28

申请号：US12667507

申请日：2007-07-04

申请人： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

发明人： Guangxin Xiang ,  Liangbin Pan ,  Wanli Xing ,  Lihua Huang ,  Zhongyao Yu ,  Jing Zhu ,  Yuxiang Zhou ,  Jing Cheng

IPC分类号： G01N33/50 ,  G01N27/26

CPC分类号： G01N33/4836 ,  B01J2219/00653 ,  B01J2219/00743 ,  B01L3/502707 ,  B01L2300/0645 ,  B01L2400/0421 ,  B01L2400/0424

摘要： A microelectrode sensing device includes a substrate and an array of microelectrode sensors. Each sensor includes a first conductive layer that at least partially conducts electricity. The first conductive layer is formed above the substrate and patterned to include a recording electrode that measures electrical activities of target cells. Each sensor also includes a second conductive layer that at least partially conducts electricity. The second conductive layer is elevated above the first layer and patterned to include multiple positioning electrodes arranged to define a sensing region above the recording electrode. The positioning electrodes are designed to generate an electric field pattern in the sensing region to move and confine the target cells to a sub-region of the sensing region that at least partially overlaps the recording electrode.

摘要翻译： 微电极感测装置包括基板和微电极传感器阵列。 每个传感器包括至少部分地导电的第一导电层。 第一导电层形成在衬底上并被图案化以包括测量靶细胞的电活动的记录电极。 每个传感器还包括至少部分地传导电的第二导电层。 第二导电层在第一层之上被升高并且被图案化以包括布置成限定记录电极上方的感测区域的多个定位电极。 定位电极被设计成在感测区域中产生电场图案以移动并将目标单元限制在至少部分地与记录电极重叠的感测区域的子区域上。