公开(公告)号：US20140349893A1

公开(公告)日：2014-11-27

申请号：US14262570

申请日：2014-04-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard HAUGLAND ,  Karen MARTIN ,  Wayne PATTON

IPC: G01N33/543

CPC classification number: G01N33/54306 ,  C07K1/1077 ,  C07K1/13 ,  G01N27/44726 ,  G01N33/581 ,  G01N33/583 ,  G01N33/6839

Abstract: The invention describes methods for labeling or detecting of immobilized poly(amino acids), including peptides, polypeptides and proteins, on membranes and other solid supports, using fluorescent dipyrrometheneboron difluoride dyes. Such immobilized poly(amino acids) are labeled or detected on blots or on arrays of poly(amino acids), or are attached to immobilized aptamers.

Abstract translation: 本发明描述了使用荧光二吡咯亚甲基二氟化物染料在膜和其它固体支持物上标记或检测固定的聚（氨基酸），包括肽，多肽和蛋白质的方法。 这种固定的聚（氨基酸）在印迹或聚（氨基酸）阵列上被标记或检测，或连接到固定的适体。

公开(公告)号：US20140038856A1

公开(公告)日：2014-02-06

申请号：US13890137

申请日：2013-05-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Aleksey RUKAVISHNIKOV ,  Courtenay HART ,  Richard HAUGLAND ,  Wai-Yee LEUNG ,  Wayne PATTON ,  Zhenjun DIWU

IPC: G01N33/58

CPC classification number: G01N33/52 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07F5/022 ,  G01N33/533 ,  G01N33/582

Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

Abstract translation: 本发明提供了通过能够选择性地结合亲和标签来促进融合蛋白的检测和标记的方法和荧光化合物。 荧光化合物具有通式A（B）n，其中A是荧光团，B是作为带电化学部分的结合结构域，其蛋白质或片段，n是1-6的整数，条件是 蛋白质或其片段不是抗体或从抗体产生。 本发明提供用于检测和标记含有多组氨酸亲和标签的融合蛋白的特异性荧光化合物和方法。 这些化合物具有通式A（L）m（B）n，其中A是荧光团，L是连接体，B是乙酸结合域，m是1至4的整数，n是1至 乙酸基团与亲和标签的带正电荷的组氨酸残基直接相互作用，以有效地标记和检测当存在于酸性或中性环境中时含有这种亲和标签的融合蛋白。

公开(公告)号：US20150031579A1

公开(公告)日：2015-01-29

申请号：US14508573

申请日：2014-10-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard HAUGLAND ,  Stephen Yue

IPC: C12Q1/68

CPC classification number: C09B23/02 ,  C12Q1/6816 ,  C12Q1/6876 ,  G01N1/30 ,  G01N27/44726 ,  G01N33/52 ,  G01N33/5308 ,  G01N33/531 ,  G01N33/582 ,  G01N33/68 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/107

Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.

Abstract translation: 描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。 当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。 描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

公开(公告)号：US20140193926A1

公开(公告)日：2014-07-10

申请号：US14080559

申请日：2013-11-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard HAUGLAND ,  Wai-Yee Leung ,  Jixiang Liu

IPC: G01N33/536

CPC classification number: G01N33/536 ,  C07C309/38 ,  C07D207/16 ,  C07D207/46 ,  C07D211/56 ,  C07D211/60 ,  C07D211/62 ,  C07D401/12 ,  C07H17/08 ,  C07H19/04 ,  C07K14/77 ,  C07K16/18 ,  C09B57/001

Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.

Abstract translation: 本发明提供了一种基于芘磺酸核的新型反应性荧光剂。 通过使反应性基团与缀合物的其他物质上的互补反应性基团反应，将药剂容易地与其它物质结合。 还提供了使用本发明化合物检测和/或定量样品中的分析物的方法。 在一个示例性实施方案中，本发明提供掺入本发明化合物的多色测定法。