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公开(公告)号：US20220390730A1
公开(公告)日：2022-12-08
申请号：US17770321
申请日：2020-10-22
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Christian SCHUMANN
Abstract: An optical system for a light-sheet microscope comprises transporting optics configured to project, into a sample, a light sheet for illuminating a sample plane positioned obliquely to an optical axis of the transporting optics and to project the illuminated sample plane into an intermediate image space. The transporting optics comprises an interchanging system that includes a first light-deflection element and a second light-deflection element. The interchanging system is configured to switch an illumination direction along which the light sheet illuminates the sample by alternately introducing the first light-deflection element and the second light-deflection element into a beam path of the transporting optics. The first light-deflection element causes a partial image inversion in only one direction. The second light-deflection element causes a complete image inversion in two directions.
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公开(公告)号：US20220373464A1
公开(公告)日：2022-11-24
申请号：US17734164
申请日：2022-05-02
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Kai RITSCHEL , Christian SCHUMANN
Abstract: A control system for automatedly determining an illumination intensity of at least one light source of a fluorescence microscope is provided. The control system is configured to automatedly determine, after a change in a light path, a control value for the illumination intensity of the at least one light source in order to achieve a desired value of an inspection parameter characterizing sample inspection. The light path comprises at least one of: an illumination path from the at least one light source to the sample and an imaging path from the sample to at least one detector. Determining the control value is based on: (i) a value of the illumination intensity that was set before the change in the light path, (ii) a value of the inspection parameter used before the change in the light path, and (iii) a physical model of the light path.
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3.发明申请公开(公告)号：US20220284604A1
公开(公告)日：2022-09-08
申请号：US17676318
申请日：2022-02-21
Applicant: Leica Microsystems CMS GmbH
Inventor： Christian SCHUMANN , Kai RITSCHEL , Mate BELJAN
Abstract: A multispectral microscope system includes: a first detector element for capturing a first image of a sample in a first spectral channel; at least a second detector element for capturing a second image of the sample in a second spectral channel; and a processor for determining a spatial correlation between the first and second images based on a spectral crosstalk between the first and second spectral channels and registering the first and second images based on the spatial correlation.
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公开(公告)号：US20240241359A1
公开(公告)日：2024-07-18
申请号：US18562393
申请日：2022-05-23
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Christian SCHUMANN
CPC classification number: G02B21/16 , G01N21/6408 , G01N21/6458 , G02B21/365
Abstract: A method for examining a sample containing a target fluorophore j using a fluorescence microscope includes acquiring a series of sample images over an acquisition time interval, and adjusting an illumination parameter Pk in a plurality of iteration steps n during the acquisition time interval to different set values. The series of sample images are acquired after adjusting the illumination parameter Pk in at least some of the plurality of iteration steps n. The method further includes determining a bleaching behaviour descriptor κj indicative of a bleaching behaviour of the target fluorophore j and a fluorescence response descriptor Ij indicative of a fluorescence response of the target fluorophore j for the set values of the illumination parameter Pk. The illumination parameter Pk is adjusted based on the bleaching behaviour descriptor κj and the fluorescence response descriptor Ij as determined in a preceding one of the plurality of iteration steps n.
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公开(公告)号：US20220390732A1
公开(公告)日：2022-12-08
申请号：US17827823
申请日：2022-05-30
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Arnold MUELLER-RENTZ , Christian SCHUMANN , Sebastian HITZLER
Abstract: A microscope for imaging a sample by a transmitted light contrasting method includes an objective lens holder configured to place an objective lens of a number of objective lenses onto an optical axis of the microscope. The microscope further includes a lens system for forming an intermediate image of an exit pupil of any one of the number of objective lenses placed onto the optical axis. The intermediate image is formed at a respective conjugated plane conjugate to the exit pupil. The microscope further includes a control device configured for automatically positioning a modulation element onto the optical axis at a positon related to the respective conjugated plane.
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Patent Agency Ranking
公开(公告)号：US20220171176A1
公开(公告)日：2022-06-02
申请号：US17594181
申请日：2020-04-08
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Patric PELZER , Christian SCHUMANN
Abstract: A device for receiving samples in a microscope is provided. The device comprises a microtiter plate. The microtiter plate comprises an inner part with cavities for the samples and with an optically transparent bottom plate, which seals the cavities underneath and a frame which defines a supporting surface and is configured to hold the inner part at least in a first position. The bottom plate is arranged above the supporting surface in the first position of the inner part. The inner part is movable relative to the frame along a direction (A) perpendicular to the supporting surface.
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公开(公告)号：US20200319659A1
公开(公告)日：2020-10-08
申请号：US16835449
申请日：2020-03-31
Applicant: Leica Microsystems CMS GmbH
Inventor： Alexander WEISS , Sebastian HITZLER , Christian SCHUMANN
Abstract: A feed device for an immersion medium for use with an objective enabling a specimen to be imaged microscopically includes a cap fitted releasably or fixedly to the objective and delimiting a receptacle space for the immersion medium. The cap has an exit opening aligned with an optical element of the objective facing the specimen. The immersion medium held in the receptacle space is feedable through the exit opening to a target space situated between the optical element of the objective and the specimen. A sensor is integrated in the cap and has an electrode structure configured to detect an amount of the immersion medium fed through the exit opening to the target space. The electrode structure at least partly encloses the exit opening and has a spatial detection region extending away from the exit opening in a radial direction.
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8.发明公开公开(公告)号：US20230324662A1
公开(公告)日：2023-10-12
申请号：US18040154
申请日：2020-08-05
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Christian SCHUMANN , Benjamin DEISSLER , Kai RITSCHEL
IPC: G02B21/00
CPC classification number: G02B21/0076 , G02B21/0032 , G02B21/0084
Abstract: A method for automatically ascertaining illumination brightnesses to be adjusted of at least two light sources for exciting at least one respective fluorophore in a sample to be imaged in a fluorescence microscope includes separately controlling, in terms of illumination brightness, each of the at least two light sources, detecting an image intensity of a microscopically imaged sample with at least two detectors, and automatically ascertaining the illumination brightnesses to be adjusted of the at least two light sources in such a way that a predefined setpoint of a signal-to-noise ratio is reached for each fluorophore. In order to ascertain the illumination brightnesses of the at least two light sources, cross-talk of a detector for different emission spectra of the fluorophores and/or cross-excitation of a fluorophore for different illumination spectra of the light sources are/is taken into account.
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公开(公告)号：US20230152562A1
公开(公告)日：2023-05-18
申请号：US17915111
申请日：2020-03-31
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Christian SCHUMANN
IPC: G02B21/00
CPC classification number: G02B21/0032 , G02B21/0044 , G02B21/008
Abstract: A light sheet microscope includes a light source configured to emit illumination light, an optical system configured to form a light sheet from the illumination light in a sample space, the light sheet being focused in a thickness direction perpendicular to a light propagation direction thereof to form a beam waist in the thickness direction, wherein the optical system has a field diaphragm adjustable to vary a width of the light sheet in a width direction, a scanning element configured to move the light sheet a scanning distance in the sample space along a scanning direction, and a control unit configured to control the field diaphragm for adjusting the width of the light sheet and to control the scanning element for moving the light sheet by the scanning distance to manipulate a target area of a sample by scanning the target area with the beam waist.
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公开(公告)号：US20210341281A1
公开(公告)日：2021-11-04
申请号：US17283573
申请日：2019-10-11
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Christian SCHUMANN
Abstract: A method for determining a tilting of a coverslip in a microscope, which has an object lens facing the coverslip, includes defining at least three measuring points which span a plane on a surface of the coverslip. The following steps are carried out for each of the measuring points: directing a measuring light beam through the object lens to the respective measuring point; producing a reflection light beam by at least partial reflection at the respective measuring point; directing the reflection light beam through the object lens onto a position-sensitive sensor and detecting an incidence position thereon; and determining a distance of the respective measuring point from the object lens along an optical axis thereof based on the detected incidence position. Based on the determined distances, a tilting of the plane spanned by the at least three measuring points relative to the optical axis is determined.
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