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公开(公告)号：US20240168274A1
公开(公告)日：2024-05-23
申请号：US18515339
申请日：2023-11-21
Applicant: Leica Microsystems CMS GmbH
Inventor： Kai RITSCHEL , Mate BELJAN
CPC classification number: G02B21/367 , G02B21/26
Abstract: Embodiments of the present invention relate to an optical imaging system, and to methods, systems, and computer programs for such an optical imaging system. The methods comprise obtaining first image data of an imaging device of the imaging system, the first image data comprising a representation of a pattern. The methods comprise obtaining second image data of the pattern from the imaging device after the pattern has been displaced by a stage of the optical imaging system by a distance in a dimension defined relative to the stage. The methods comprise determining an offset between the patterns of the first and second image data in two dimensions. The methods comprise calculating a conversion parameter based on the offset and the distance. A first method comprises controlling a drive unit configured to displace the stage based on the conversion parameter.
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公开(公告)号：US20210149175A1
公开(公告)日：2021-05-20
申请号：US17082320
申请日：2020-10-28
Applicant: Leica Microsystems CMS GmbH
Inventor： Kai RITSCHEL , Marco BINGEL , Patric PELZER
Abstract: An optical imaging device for a microscope comprises a first optical system configured to form a first optical image corresponding to a first region of a sample in accordance with a first imaging mode, a second optical system configured to form a second optical image corresponding to a second region of said sample, wherein said first and second regions spatially coincide in a target region of said sample and said first and second imaging modes are different from each other, a memory storing first distortion correction data suitable for correcting a first optical distortion caused by said first optical system in said first optical image, second distortion correction data suitable for correcting a second optical distortion caused by said second optical system in said second optical image, and transformation data suitable for correcting positional misalignment between said first and second optical images, and a processor which is configured to process first image data representing said first optical image based on said first distortion correction data for generating first distortion corrected image data, to process second image data representing said second optical image based on said second distortion correction data for generating second distortion corrected image data; and to combine said first and second distortion corrected image data based on said transformation data for generating combined image data representing a combined image which corresponds to said target region of said object.
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公开(公告)号：US20240053596A1
公开(公告)日：2024-02-15
申请号：US18267117
申请日：2021-11-30
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Oliver KEUL , Volker SCHACHT , Kai RITSCHEL
CPC classification number: G02B21/365 , G02B21/26 , G02B21/242
Abstract: A control device for a microscope includes an actuator configured to shift a microscopic field of view relative to a sample, and an operating device configured to be operated by a user to control the actuator in accordance with a response characteristic determining a shift sensitivity. The field of view is shifted relative to the sample in response to a user operation of the operating device. The control device further includes a processor configured to determine a total visual magnification, and to control the response characteristic of the operating device based on the total visual magnification. The field of view is visualized by the microscope to the user based on the total visual magnification.
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公开(公告)号：US20230175963A1
公开(公告)日：2023-06-08
申请号：US17994434
申请日：2022-11-28
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Benjamin DEISSLER , Christian SCHUMANN , Kai RITSCHEL , Mate BELJAN , Wernher FOUQUET , Gheorghe COJOC
IPC: G01N21/64
CPC classification number: G01N21/64 , G01N2201/127
Abstract: A method of analyzing a mixed fluorescence response of a plurality of fluorophores in a microscopic sample includes reconstructing individual fluorescence responses from a mixed fluorescence response using spectral un-mixing based on reference emission spectra for fluorophores to be reconstructed, and a procedure for determining and validating reference emission spectra including providing a plurality of image acquisition settings for a sequence of images of the sample equal to, or greater than, the plurality of fluorophores and including an illumination setting for each image, acquiring the sequence of images using the plurality of image acquisition settings and storing each image together with the corresponding illumination setting, determining candidate reference emission spectra for the fluorophores to be reconstructed from the sequence of images of the sample using one or more reference emission spectra determination algorithms, and conditionally using the candidate reference emission spectra as the reference emission spectra in the spectral un-mixing.
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5.发明公开公开(公告)号：US20230324662A1
公开(公告)日：2023-10-12
申请号：US18040154
申请日：2020-08-05
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Christian SCHUMANN , Benjamin DEISSLER , Kai RITSCHEL
IPC: G02B21/00
CPC classification number: G02B21/0076 , G02B21/0032 , G02B21/0084
Abstract: A method for automatically ascertaining illumination brightnesses to be adjusted of at least two light sources for exciting at least one respective fluorophore in a sample to be imaged in a fluorescence microscope includes separately controlling, in terms of illumination brightness, each of the at least two light sources, detecting an image intensity of a microscopically imaged sample with at least two detectors, and automatically ascertaining the illumination brightnesses to be adjusted of the at least two light sources in such a way that a predefined setpoint of a signal-to-noise ratio is reached for each fluorophore. In order to ascertain the illumination brightnesses of the at least two light sources, cross-talk of a detector for different emission spectra of the fluorophores and/or cross-excitation of a fluorophore for different illumination spectra of the light sources are/is taken into account.
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Patent Agency Ranking
公开(公告)号：US20230161143A1
公开(公告)日：2023-05-25
申请号：US18057773
申请日：2022-11-22
Applicant: Leica Microsystems CMS GmbH
Inventor： Kai RITSCHEL , Benjamin DEISSLER , Dennis JAEHNERT
CPC classification number: G02B21/0076 , G01N21/6458 , G01N2021/0106
Abstract: A fluorescence microscope system for imaging a sample having at least two different fluorophores includes an illumination system configured to emit illumination light for exciting the fluorophores; an optical detection system configured to generate images of the sample based on fluorescence light emitted by the excited fluorophores; and a control unit configured to determine whether to image the sample in a concurrent imaging mode or in a sequential imaging mode, based on at least one characteristic of each of the fluorophores and based on at least one parameter of the optical detection system and/or the illumination system. In the concurrent imaging mode, the fluorophores are imaged simultaneously, and in the sequential imaging mode, the fluorophores are divided into a first group and at least one second group, and fluorophores of the first group and the at least one second group are imaged subsequently.
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公开(公告)号：US20240095959A1
公开(公告)日：2024-03-21
申请号：US18469577
申请日：2023-09-19
Applicant: Leica Microsystems CMS GmbH
Inventor： Kai RITSCHEL , Mate BELJAN
IPC: G06T7/80
CPC classification number: G06T7/80 , G06T2207/10056
Abstract: A stage insert is provided. The stage insert comprises a first region for accommodating a sample holder. Further, the stage insert comprises a second region comprising a first calibration target for calibrating a first parameter of the microscope system. The stage insert also comprises a third region comprising a second calibration target for calibrating a second parameter of the microscope system.
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公开(公告)号：US20230196513A1
公开(公告)日：2023-06-22
申请号：US18065010
申请日：2022-12-13
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Kai RITSCHEL , Oliver SCHLICKER
CPC classification number: G06T5/001 , G01N21/6458 , G06T2207/10024 , G06T2207/10056 , G06T2207/10064
Abstract: A fluorescence microscope system including an optical detection system configured to capture a raw image of a sample, the raw image including a plurality of pixels, each pixel having a brightness value and a processor, configured to determine one or more invalid pixels in the raw image, assign a predetermined value to each invalid pixel, determine a range of brightness values including the brightness values of a majority of the plurality of pixels excluding the one or more invalid pixels, and generate a processed image of the sample based on the determined range of brightness values. (FIG. 1)
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公开(公告)号：US20220373464A1
公开(公告)日：2022-11-24
申请号：US17734164
申请日：2022-05-02
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Kai RITSCHEL , Christian SCHUMANN
Abstract: A control system for automatedly determining an illumination intensity of at least one light source of a fluorescence microscope is provided. The control system is configured to automatedly determine, after a change in a light path, a control value for the illumination intensity of the at least one light source in order to achieve a desired value of an inspection parameter characterizing sample inspection. The light path comprises at least one of: an illumination path from the at least one light source to the sample and an imaging path from the sample to at least one detector. Determining the control value is based on: (i) a value of the illumination intensity that was set before the change in the light path, (ii) a value of the inspection parameter used before the change in the light path, and (iii) a physical model of the light path.
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10.发明申请公开(公告)号：US20220284604A1
公开(公告)日：2022-09-08
申请号：US17676318
申请日：2022-02-21
Applicant: Leica Microsystems CMS GmbH
Inventor： Christian SCHUMANN , Kai RITSCHEL , Mate BELJAN
Abstract: A multispectral microscope system includes: a first detector element for capturing a first image of a sample in a first spectral channel; at least a second detector element for capturing a second image of the sample in a second spectral channel; and a processor for determining a spatial correlation between the first and second images based on a spectral crosstalk between the first and second spectral channels and registering the first and second images based on the spatial correlation.
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