公开(公告)号：US20160202262A1

公开(公告)日：2016-07-14

申请号：US15066118

申请日：2016-03-10

Applicant: Korea University Research and Business Foundation

Inventor： Man Bock Gu ,  Jee Woong Park ,  Tatavarty Rameshwar

IPC: G01N33/574 ,  G01N33/53 ,  B01D15/38 ,  C12N9/10

CPC classification number: G01N33/57488 ,  B01D15/3823 ,  C12N7/00 ,  C12N9/1048 ,  C12N15/1048 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/351 ,  C12N2320/13 ,  C12N2330/30 ,  C12N2770/24351 ,  C12Q1/6811 ,  C12Y204/02012 ,  G01N33/5308 ,  G01N33/56983 ,  G01N33/57407 ,  G01N33/6893 ,  G01N2333/183 ,  G01N2333/91142 ,  C12Q2525/205 ,  C12Q2541/101

Abstract: Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.

Abstract translation: 提供了基于没有靶固定的石墨烯的适体筛选方法和从该方法获得的适体，更具体地说，没有目标固定的新的GO-SELEX方法，其中单链核酸库可以与未结合的靶材料反应 或反靶物质，之后可以通过使用石墨烯分离未结合靶或对靶的单链核酸。 此外，通过上述方法获得的特异性适体可用于诊断目标相关疾病。

公开(公告)号：US09562907B2

公开(公告)日：2017-02-07

申请号：US15066514

申请日：2016-03-10

Applicant: Korea University Research and Business Foundation

Inventor： Man Bock Gu ,  Jee Woong Park ,  Tatavarty Rameshwar

IPC: C07H21/04 ,  G01N33/574 ,  C12N15/115 ,  C12N7/00 ,  B01D15/38 ,  C12N9/10 ,  G01N33/53 ,  C12N15/10 ,  C12Q1/68 ,  G01N33/569 ,  G01N33/68

CPC classification number: G01N33/57488 ,  B01D15/3823 ,  C12N7/00 ,  C12N9/1048 ,  C12N15/1048 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/351 ,  C12N2320/13 ,  C12N2330/30 ,  C12N2770/24351 ,  C12Q1/6811 ,  C12Y204/02012 ,  G01N33/5308 ,  G01N33/56983 ,  G01N33/57407 ,  G01N33/6893 ,  G01N2333/183 ,  G01N2333/91142 ,  C12Q2525/205 ,  C12Q2541/101

Abstract: Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.

Abstract translation: 提供了基于没有靶固定的石墨烯的适体筛选方法和从该方法获得的适体，更具体地说，没有目标固定的新的GO-SELEX方法，其中单链核酸库可以与未结合的靶材料反应 或反靶物质，之后可以通过使用石墨烯分离未结合靶或对靶的单链核酸。 此外，通过上述方法获得的特异性适体可用于诊断目标相关疾病。

公开(公告)号：US20160202259A1

公开(公告)日：2016-07-14

申请号：US15066514

申请日：2016-03-10

Applicant: Korea University Research and Business Foundation

Inventor： Man Bock Gu ,  Jee Woong Park ,  Tatavarty Rameshwar

IPC: G01N33/569 ,  C12N15/115 ,  C12N7/00

CPC classification number: G01N33/57488 ,  B01D15/3823 ,  C12N7/00 ,  C12N9/1048 ,  C12N15/1048 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/351 ,  C12N2320/13 ,  C12N2330/30 ,  C12N2770/24351 ,  C12Q1/6811 ,  C12Y204/02012 ,  G01N33/5308 ,  G01N33/56983 ,  G01N33/57407 ,  G01N33/6893 ,  G01N2333/183 ,  G01N2333/91142 ,  C12Q2525/205 ,  C12Q2541/101

Abstract: Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.