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公开(公告)号：US07846717B2
公开(公告)日：2010-12-07
申请号：US10525756
申请日：2003-08-26
Applicant: Kenji Yasuda , Takanori Ichiki , Kazunori Okano
Inventor： Kenji Yasuda , Takanori Ichiki , Kazunori Okano
Abstract: A microchamber including a glass substrate which is transparent to a specific wavelength, an absorbent region which absorbs the specific wavelength, and a melting substance region which does not absorb the specific wavelength, is solid at room temperature and melts when heated, which regions are layered on the glass substrate. The absorbent region, is irradiated with a focused light beam of the specific wavelength and locally heated in the vicinity of the converging rays, so that the melting substance region is locally melted at a portion adjacent to the absorbent region, thereby forming a cavity as the focused light beam moves. Accordingly, the shape of the microchamber can be arbitrarily changed in accordance with the process of cell culture.
Abstract translation: 包括对特定波长透明的玻璃基板，吸收特定波长的吸收区域和不吸收特定波长的熔融物质区域的微室在室温下为固体，在加热时熔融，哪些区域分层 在玻璃基板上。 利用特定波长的聚焦光照射吸收区域，并在会聚射线附近局部加热，使得熔融物质区域在与吸收区域相邻的部分局部熔融，从而形成为空腔 聚焦光束移动。 因此，可以根据细胞培养的过程任意地改变微室的形状。
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公开(公告)号：US20060039828A1
公开(公告)日：2006-02-23
申请号：US10525521
申请日：2003-08-26
Applicant: Kenji Yasuda , Takanori Ichiki , Kazunori Okano
Inventor： Kenji Yasuda , Takanori Ichiki , Kazunori Okano
IPC: G01N21/00
CPC classification number: B01F13/0071 , B01F13/0076 , B01L3/502792 , B01L2300/089 , B01L2400/0415 , G01N15/1468 , G01N35/00029 , G01N2015/149 , G01N2035/0097 , Y10T436/2575
Abstract: A droplet operation device having a substrate having a light transmissibility and subjected to a water repellent treatment, a means for supplying droplets onto the substrate, a means for transporting the droplets on the water repellent substrate, and a means for measuring the state of the droplets, comprising the light transmittable substrate (11) receiving the water repellent treatment on the surface thereof, solvent feeding ports (21), (22), and (23) for leading reagent or specimen droplets (51) onto the substrate (11), optical measuring units (61), (62), and (63), and electric field application units (71), (72), and (73), whereby the inside of the droplets can be observed with a microscope, and the results of the reaction of the droplets against the other droplets can be measured and classified by stopping and moving the droplets in any direction.
Abstract translation: 一种液滴操作装置，具有具有透光性且进行防水处理的基板，用于将液滴供给到基板上的装置，用于在憎水基板上输送液滴的装置，以及用于测量液滴状态的装置 包括在其表面上接受防水处理的透光性基材（11），将试剂或试样液滴（51）引导到基材（11）上的溶剂供给口（21），（22）和（23） 光学测量单元（61），（62）和（63）以及电场施加单元（71），（72）和（73），由此可以用显微镜观察液滴的内部，结果 可以通过在任何方向上停止和移动液滴来测量和分类液滴对其他液滴的反应。
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公开(公告)号：US07597850B2
公开(公告)日：2009-10-06
申请号：US10525521
申请日：2003-08-26
Applicant: Kenji Yasuda , Takanori Ichiki , Kazunori Okano
Inventor： Kenji Yasuda , Takanori Ichiki , Kazunori Okano
IPC: B01L3/02
CPC classification number: B01F13/0071 , B01F13/0076 , B01L3/502792 , B01L2300/089 , B01L2400/0415 , G01N15/1468 , G01N35/00029 , G01N2015/149 , G01N2035/0097 , Y10T436/2575
Abstract: A droplet operation device has a substrate with light transmissibility and having been subjected to a water repellent treatment, a way for supplying droplets onto the substrate, a way for transporting the droplets on the water repellent substrate, and a way for measuring the state of the droplets. The droplet operation device includes the light transmittable substrate, solvent feeding ports for leading reagent or specimen droplets onto the substrate, optical measuring units, and electric field application units. As a result, the inside of the droplets can be observed with a microscope, and results of the reaction of the droplets against other droplets can be measured and classified by stopping and moving the droplets in any direction.
Abstract translation: 液滴操作装置具有透光性的基板，并进行防水处理，将液滴供给到基板上的方式，用于输送憎水基板上的液滴的方式，以及测定 液滴。 液滴操作装置包括可透光基板，用于将试剂或试样液滴引导到基板上的溶剂供给口，光学测量单元和电场施加单元。 结果，可以用显微镜观察液滴的内部，并且可以通过在任何方向上停止和移动液滴来测量和分类液滴对其它液滴的反应结果。
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公开(公告)号：US20060183118A1
公开(公告)日：2006-08-17
申请号：US10525404
申请日：2003-08-26
Applicant: Kenji Yasuda , Takanori Ichiki , Kazunori Okano
Inventor： Kenji Yasuda , Takanori Ichiki , Kazunori Okano
Abstract: On a glass plate being transparent at specified wavelengths there are provided a laminated region being absorptive at the specified wavelengths; means for applying voltage to the region, the region exhibiting electrical conductivity; means for binding nucleic acids onto the region; a container for accommodating cells on the region; means for culturing cells in the container; means for observing the cells; and means for effecting localized dissociation and recovery of nucleic acid components bound on the region by heat, the heat generated locally only in the vicinity of focused light by irradiating the region with focused light of the specified wavelengths. For clarifying the distribution of nucleic acid components in cells of specified condition or the distribution of nucleic acid components in each cell of a tissue cell mass there is provided means for selectively separating and recovering nucleic acid components of specified range in each cell of specified cellular condition.
Abstract translation: 在特定波长透明的玻璃板上，提供在特定波长下吸收的层叠区域; 用于向该区域施加电压的装置，显示导电性的区域; 用于将核酸结合到该区域上的方法; 用于容纳所述区域上的细胞的容器; 用于在容器中培养细胞的方法; 观察细胞的手段; 以及用于通过加热在区域上结合的核酸组分的局部解离和回收的手段，通过用指定波长的聚焦光照射该区域，局部仅在聚焦光附近产生的热。 为了澄清核酸组分在特定条件的细胞中的分布或组织细胞块的每个细胞中的核酸组分的分布，提供了用于在特定细胞条件的每个细胞中选择性分离和回收特定范围的核酸组分的手段 。
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公开(公告)号：US20060014273A1
公开(公告)日：2006-01-19
申请号：US10525756
申请日：2003-08-26
Applicant: Kenji Yasuda , Takanori Ichiki , Kazunori Okano
Inventor： Kenji Yasuda , Takanori Ichiki , Kazunori Okano
IPC: C12M3/06
Abstract: A microchamber comprising a glass substrate (101) which is transparent to a specific wavelength, an absorbent region (102) which absorbs the specific wavelength, and a melting substance region (103) which does not absorb the specific wavelength, is solid at room temperature and melts when heated, which regions are layered on the glass substrate. The absorbent region, is irradiated with a focused light beam (402) of the specific wavelength and locally heated in the vicinity of the converging rays, so that the melting substance region (103) is locally melted at a portion adjacent to the absorbent region, thereby forming a cavity (403) as the focused light beam moves. Accordingly, the shape of the microchamber can be arbitrarily changed in accordance with the process of cell culture.
Abstract translation: 一种微室，包括对特定波长透明的玻璃基板（101），吸收特定波长的吸收区域（102）和不吸收特定波长的熔融物质区域（103）在室温下为固体 并且在加热时熔融，哪些区域层叠在玻璃基板上。 吸收区域被聚焦光束（402）照射，并在会聚射线附近被局部加热，使得熔融物质区域（103）在与吸收区域相邻的部分局部熔化， 从而在聚焦光束移动时形成空腔（403）。 因此，可以根据细胞培养的过程任意地改变微室的形状。
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Patent Agency Ranking
公开(公告)号：US20060073076A1
公开(公告)日：2006-04-06
申请号：US10525875
申请日：2003-08-26
Applicant: Takanori Ichiki , Kenji Yasuda
Inventor： Takanori Ichiki , Kenji Yasuda
IPC: G01N33/48
CPC classification number: G01N15/147 , G01N15/1484 , G01N2015/149
Abstract: A cell analysis and sorting apparatus, comprising a channel into which a fluid containing samples is introduced, the samples being introduced by a laminar flow into a sample-separating portion; a pair of fluid passages arranged symmetrically on both sides of the channel, a pair of streams of fluid that are made to meet in the sample-separating portion being introduced into the fluid passages; means for introducing an external force to the sample-separating portion only when an observed sample is discharged out of the sample-separating portion; a sample recovery channel disposed downstream of the channel into which the samples are introduced such that the fluid containing a sample selected from the sample-selecting portion flows out in a laminar flow; and a pair of fluid passages which are arranged symmetrically on both sides of the sample recovery channel and into which unwanted samples are discharged, whereby the collected samples can be prevented from being damaged by sorting the samples based on the micro structure of the samples and a fluorescence distribution in the samples.
Abstract translation: 一种细胞分析和分选装置，包括其中引入含有样品的流体的通道，所述样品通过层流引入样品分离部分; 一对流体通道对称地设置在通道的两侧，使得在样品分离部分中相遇的一对流体流被引入到流体通道中; 只有当观察到的样品从样品分离部分排出时才将样品分离部分引入外力的装置; 样品回收通道，其设置在所述通道的下游，所述样品被引入所述通道中，使得包含选自所述样品选择部分的样品的流体以层流流出; 以及一对流体通道，其对称地布置在样品回收通道的两侧，并且排出不想要的样品，由此可以基于样品的微结构对样品进行分选而防止收集的样品被损坏，并且 样品中的荧光分布。
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公开(公告)号：US08663572B2
公开(公告)日：2014-03-04
申请号：US10590969
申请日：2004-07-22
Applicant: Takanori Ichiki
Inventor： Takanori Ichiki
IPC: B01J19/08
CPC classification number: H05H1/46 , H05H2001/463
Abstract: The present invention provides a microplasma jet generator capable of stably generating a microplasma jet in a microspace at atmospheric pressure with low electric power.The microplasma jet generator is driven with a VHF power supply to generate an inductively coupled microplasma jet and includes a substrate, a micro-antenna disposed on the substrate, and a discharge tube located close to the micro-antenna. The micro-antenna has a flat meandering shape with plural turns.
Abstract translation: 本发明提供一种能够在低功率的大气压下在微空间中稳定地产生微血管射流的微血管射流发生器。 微型喷气发生器由VHF电源驱动以产生电感耦合微等离子体射流，并且包括衬底，设置在衬底上的微型天线和位于微天线附近的放电管。 微型天线具有多匝平坦的曲折形状。
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公开(公告)号：US08592348B2
公开(公告)日：2013-11-26
申请号：US12447803
申请日：2007-11-01
Applicant: Naoto Nemoto , Takanori Ichiki , Manish Biyani
Inventor： Naoto Nemoto , Takanori Ichiki , Manish Biyani
IPC: C40B50/14
CPC classification number: B01J19/0046 , B01J2219/00317 , B01J2219/00585 , B01J2219/00592 , B01J2219/00596 , B01J2219/00599 , B01J2219/00608 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725
Abstract: A method for producing a chip on which biomolecules are immobilized in an aligned state, comprises (a) producing a substrate 1 on which a plurality of biomolecules 1 of a single type are immobilized in an aligned state, (b) adding reaction reagents for synthesizing biomolecules 2 to microreactors on a microreactor chip comprising the microreactors at positions overlapping with the sequence positions of the biomolecules 1 immobilized on the substrate 1 produced in step (a), (c) closely attaching the microreactor chip to the substrate 1 so that the reaction reagents for synthesizing the biomolecules 2 are allowed to come into contact with the biomolecules 1, so as to synthesize the biomolecules 2 in the microreactors, and (d) superposing the microreactor chip on a substrate 2 after completion of step (c) so as to bind the biomolecules 2 onto the substrate 2; and a chip produced thereby.
Abstract translation: 一种制备其中以对准状态固定有生物分子的芯片的方法，包括（a）制备在其上固定有多种单一生物分子1的底物1，（b）加入用于合成的反应试剂 在微反应器芯片上的生物分子2与在步骤（a）中制备的固定在基板1上的生物分子1的序列位置重叠的位置处的微反应器，（c）将微反应器芯片紧密连接到基板1，使得反应 允许用于合成生物分子2的试剂与生物分子1接触，以便在微反应器中合成生物分子2，和（d）在步骤（c）完成后将微反应器芯片叠加在基底2上，以便 将生物分子2结合到基底2上; 和由此制造的芯片。
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公开(公告)号：US20100035769A1
公开(公告)日：2010-02-11
申请号：US12447803
申请日：2007-11-01
Applicant: Naoto Nemoto , Takanori Ichiki , Manish Biyani
Inventor： Naoto Nemoto , Takanori Ichiki , Manish Biyani
CPC classification number: B01J19/0046 , B01J2219/00317 , B01J2219/00585 , B01J2219/00592 , B01J2219/00596 , B01J2219/00599 , B01J2219/00608 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725
Abstract: It is an object of the present invention to provide a method for producing a biomolecule assay chip using a microreactor technique, and a chip produced by the method.The present invention provides: a method for producing a chip on which biomolecules are immobilized in an aligned state, which comprises (a) a step of producing a substrate 1 on which a plurality of biomolecules 1 of a single type are immobilized in an aligned state, (b) a step of adding reaction reagents for synthesizing biomolecules 2 to microreactors on a microreactor chip comprising the microreactors at positions overlapping with the sequence positions of the biomolecules 1 immobilized on the substrate 1 produced in the step (a), (c) a step of closely attaching the microreactor chip to the substrate 1 so that the reaction reagents for synthesizing the biomolecules 2 are allowed to come into contact with the biomolecules 1, so as to synthesize the biomolecules 2 in the microreactors, and (d) superposing the microreactor chip on a substrate 2 after completion of the step (c) so as to bind the biomolecules 2 onto the substrate 2; and a chip produced by the aforementioned method.
Abstract translation: 本发明的目的是提供使用微反应器技术生产生物分子测定芯片的方法和通过该方法制造的芯片。 本发明提供一种制备其中以对准状态固定生物分子的芯片的方法，其包括（a）制备其上以一定类型的多个生物分子1以对准状态固定的基板1的步骤 （b）在步骤（a），（c）中生产的固定在基板1上的生物分子1的序列位置重叠的位置上，在包含微反应器的微反应器芯片上的微反应器上加入用于合成生物分子2的反应试剂的步骤， 将微反应器芯片紧密地附接到基板1上，使得用于合成生物分子2的反应试剂与生物分子1接触，从而在微反应器中合成生物分子2，并且（d） 在步骤（c）完成之后在基板2上的微反应器芯片，以将生物分子2结合到基板2上; 以及通过上述方法制造的芯片。
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公开(公告)号：US20090116166A1
公开(公告)日：2009-05-07
申请号：US12299859
申请日：2007-04-06
Applicant: Masashi Matsumori , Shigeki Nakatsuka , Takanori Ichiki
Inventor： Masashi Matsumori , Shigeki Nakatsuka , Takanori Ichiki
CPC classification number: H05H1/46 , H05H2001/4667 , H05H2001/4682
Abstract: An apparatus for generating atmospheric-pressure plasma includes: a substrate; an antenna arranged on the substrate; a discharge tube arranged in the vicinity of the antenna; a high-frequency power supply for supplying VHF band high-frequency power to the antenna; and a matching circuit for receiving a high frequency from the high-frequency power supply and adjusting a reflection wave. In this apparatus for generating atmospheric-pressure plasma, a phase circuit is connected between the matching circuit and the antenna, and the phase circuit has a circuit constant setting such that a position of a maximum value of a current amplitude of a standing wave or a position of a minimum value of a voltage amplitude of the standing wave is in the vicinity of the antenna. This configuration can efficiently generate plasma and reduce the size of the apparatus.
Abstract translation: 用于产生大气压等离子体的装置包括：基板; 布置在所述基板上的天线; 布置在天线附近的放电管; 用于向天线提供VHF频带高频电力的高频电源; 以及用于从高频电源接收高频并调整反射波的匹配电路。 在用于产生大气压等离子体的装置中，相位电路连接在匹配电路和天线之间，相位电路具有电路常数设定，使得驻波的电流幅度的最大值或 驻波的电压振幅的最小值的位置在天线附近。 该配置可以有效地产生等离子体并减小设备的尺寸。
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