It is an object of the present invention to provide a method for producing a biomolecule assay chip using a microreactor technique, and a chip produced by the method.The present invention provides: a method for producing a chip on which biomolecules are immobilized in an aligned state, which comprises (a) a step of producing a substrate 1 on which a plurality of biomolecules 1 of a single type are immobilized in an aligned state, (b) a step of adding reaction reagents for synthesizing biomolecules 2 to microreactors on a microreactor chip comprising the microreactors at positions overlapping with the sequence positions of the biomolecules 1 immobilized on the substrate 1 produced in the step (a), (c) a step of closely attaching the microreactor chip to the substrate 1 so that the reaction reagents for synthesizing the biomolecules 2 are allowed to come into contact with the biomolecules 1, so as to synthesize the biomolecules 2 in the microreactors, and (d) superposing the microreactor chip on a substrate 2 after completion of the step (c) so as to bind the biomolecules 2 onto the substrate 2; and a chip produced by the aforementioned method.