公开(公告)号：US08481770B2

公开(公告)日：2013-07-09

申请号：US13497157

申请日：2010-09-17

Applicant: Ken Sugo

Inventor： Ken Sugo

IPC: C07F9/02

CPC classification number: B01D15/08 ,  A23J7/00 ,  B01D15/426 ,  B01J20/048 ,  B01J20/282 ,  B01J2220/54 ,  C11B7/00

Abstract: A method of selectively separating a phospholipid from a sample solution containing the phospholipid is provided. The method comprises: adsorbing calcium ions to a filler, at least a surface of the filler being constituted of a calcium phosphate-based compound; supplying the sample solution into an apparatus having a filling space, wherein the filling space being filled with the filler so that the phospholipid contained in the sample solution is adsorbed to the filler through the calcium ions; supplying an organic solvent-based eluate into the filling space of the apparatus to obtain a liquid containing the phospholipid and discharged from the apparatus; and fractionating the obtained liquid per a predetermined amount to thereby separate the phospholipid from the sample solution.

Abstract translation: 提供了从含有磷脂的样品溶液中选择性分离磷脂的方法。 该方法包括：将钙离子吸附到填料中，填料的至少表面由磷酸钙基化合物构成; 将样品溶液供给到具有填充空间的装置中，其中填充空间填充有填料，使得样品溶液中所含的磷脂通过钙离子吸附到填料上; 将有机溶剂型洗脱液供给到装置的填充空间中以获得含有磷脂并从该装置排出的液体; 并将所得液体按预定量进行分馏，从而将磷脂与样品溶液分离。

公开(公告)号：US20120178950A1

公开(公告)日：2012-07-12

申请号：US13497157

申请日：2010-09-17

Applicant: Ken Sugo

Inventor： Ken Sugo

IPC: C11B3/10

CPC classification number: B01D15/08 ,  A23J7/00 ,  B01D15/426 ,  B01J20/048 ,  B01J20/282 ,  B01J2220/54 ,  C11B7/00

Abstract: A method of selectively separating a phospholipid from a sample solution containing the phospholipid is provided. The method comprises: adsorbing calcium ions to a filler, at least a surface of the filler being constituted of a calcium phosphate-based compound; supplying the sample solution into an apparatus having a filling space, wherein the filling space being filled with the filler so that the phospholipid contained in the sample solution is adsorbed to the filler through the calcium ions; supplying an organic solvent-based eluate into the filling space of the apparatus to obtain a liquid containing the phospholipid and discharged from the apparatus; and fractionating the obtained liquid per a predetermined amount to thereby separate the phospholipid from the sample solution.

Abstract translation: 提供了从含有磷脂的样品溶液中选择性分离磷脂的方法。 该方法包括：将钙离子吸附到填料上，填料的至少表面由磷酸钙基化合物构成; 将样品溶液供给到具有填充空间的装置中，其中填充空间填充有填料，使得样品溶液中所含的磷脂通过钙离子吸附到填料上; 将有机溶剂型洗脱液供给到装置的填充空间中以获得含有磷脂并从该装置排出的液体; 并将所得液体按预定量进行分馏，从而将磷脂与样品溶液分离。

公开(公告)号：US20060024823A1

公开(公告)日：2006-02-02

申请号：US11190868

申请日：2005-07-28

Applicant: Tsuyoshi Ishikawa ,  Ken Sugo

Inventor： Tsuyoshi Ishikawa ,  Ken Sugo

IPC: C12M1/00 ,  C12N5/00

CPC classification number: C12N5/0068 ,  C12N2533/18

Abstract: Cell culture carriers that allow cells to efficiently adhere thereto and sufficiently grow thereon and from which the grown cells can be easily removed or detached, a method for manufacturing cell culture carriers that enables to easily and reliably manufacture such cell culture carriers and a method for culturing cells using such cell culture carriers are disclosed. The cell culture carrier includes a base material having a granular shape and a coating layer that is provided so as to cover the surface of the base material, the coating layer is mainly made of calcium phosphate-based apatite in which a part of calcium is deficient. Such cell culture carriers are utilized in cell culture in which cells adhere to and grow on the surface of cell culture carriers, particularly in three-dimensional high-density culture (suspension culture). The Ca deficiency rate in the calcium phosphate-based apatite in which a part of calcium is deficient is preferably in the range of 1 to 30 mol %.

Abstract translation: 细胞培养载体，其允许细胞有效地附着于其上并在其上充分生长，并且可以从其中容易地去除或分离生长的细胞;制造能够容易且可靠地制造这种细胞培养载体的细胞培养载体的方法和培养方法 公开了使用这种细胞培养载体的细胞。 细胞培养载体包括具有颗粒形状的基材和设置成覆盖基材表面的涂层，涂层主要由磷酸钙基磷灰石制成，其中钙的一部分不足 。 这种细胞培养载体用于细胞培养，其中细胞粘附并生长在细胞培养载体的表面上，特别是在三维高密度培养（悬浮培养）中。 一部分钙缺乏的磷酸钙磷灰石中的Ca缺乏率优选为1〜30摩尔％。

公开(公告)号：US07399836B2

公开(公告)日：2008-07-15

申请号：US11201379

申请日：2005-08-11

Applicant: Akira Yamamoto ,  Ken Sugo

Inventor： Akira Yamamoto ,  Ken Sugo

IPC: C07K16/00

CPC classification number: G01N33/551 ,  G01N33/54393

Abstract: Carrier and method of its manufacture having immobilized antigens or antibodies, including a carrier having a surface, in which at least the surface of the carrier is formed of a calcium phosphate based compound; antiligands provided on and surrounding the surface of the carrier; a blocking layer formed of a protein having low interaction with antigens or antibodies, the blocking layer being formed on a portion of the surface of the carrier where the antiligands are not provided; and antigens or antibodies each having a portion that is irrelevant to the antigen-antibody reaction on which a ligand is provided, the antigens or antibodies being immobilized to the surface of the carrier through the bonding between the ligands and the antiligands with the blocking layer effectively preventing antigens or antibodies from being directly absorbed to the surface without bonding between the ligands and the antiligands.

Abstract translation: 载体及其制造方法具有固定化的抗原或抗体，包括具有表面的载体，其中载体的至少表面由磷酸钙基化合物形成; 提供在载体表面上并围绕载体表面的抗配偶; 由与抗原或抗体相互作用低的蛋白质形成的阻断层，所述阻挡层形成在载体表面上未设置反配位体的部分上; 以及各自具有与其上提供配体的抗原 - 抗体反应无关的部分的抗原或抗体，所述抗原或抗体通过具有阻断层的配体和抗配配基之间的键合而被固定在载体的表面上 防止抗原或抗体直接吸收到表面，而不会在配体与抗配糖之间粘合。

公开(公告)号：US20060051342A1

公开(公告)日：2006-03-09

申请号：US11201379

申请日：2005-08-11

Applicant: Akira Yamamoto ,  Ken Sugo

Inventor： Akira Yamamoto ,  Ken Sugo

IPC: A61K39/395 ,  G01N1/28 ,  G01N33/53

CPC classification number: G01N33/551 ,  G01N33/54393

Abstract: Carrier and method of its manufacture having immobilized antigens or antibodies, including a carrier having a surface, in which at least the surface of the carrier is formed of a calcium phosphate based compound; antiligands provided on and surrounding the surface of the carrier; a blocking layer formed of a protein having low interaction with antigens or antibodies, the blocking layer being formed on a portion of the surface of the carrier where the antiligands are not provided; and antigens or antibodies each having a portion that is irrelevant to the antigen-antibody reaction on which a ligand is provided, the antigens or antibodies being immobilized to the surface of the carrier through the bonding between the ligands and the antiligands with the blocking layer effectively preventing antigens or antibodies from being directly absorbed to the surface without bonding between the ligands and the antiligands.

Abstract translation: 载体及其制造方法具有固定化的抗原或抗体，包括具有表面的载体，其中载体的至少表面由磷酸钙基化合物形成; 提供在载体表面上并围绕载体表面的抗配偶; 由与抗原或抗体相互作用低的蛋白质形成的阻断层，所述阻挡层形成在载体表面上未设置反配位体的部分上; 以及各自具有与其上提供配体的抗原 - 抗体反应无关的部分的抗原或抗体，所述抗原或抗体通过具有阻断层的配体和抗配配基之间的键合而被固定在载体的表面上 防止抗原或抗体直接吸收到表面，而不会在配体与抗配糖之间粘合。