-
公开(公告)号：US4410338A
公开(公告)日：1983-10-18
申请号：US388577
申请日：1982-06-15
Applicant: Minoru Yamamoto , Masana Hirai , Jiro Sakata
Inventor： Minoru Yamamoto , Masana Hirai , Jiro Sakata
CPC classification number: B01D69/127 , C08G77/06
Abstract: A gas separating member of the present invention comprises a porous substrate in the form of a film, wall or hollow fiber and a polymer film formed on a surface of the substrate by plasma polymerization. The gas separation factor (O.sub.2 /N.sub.2) ranges from 2.3 to 3.9 with the corresponding gas permeability ranging from 12 to 0.16 liter/min. m.sup.2 atm. pres.-air.A modified gas separating member of the present invention, which comprises a porous substrate in the aforementioned form and two polymer films formed on a surface of the substrate by plasma polymerization, has the gas separation factor (He/H.sub.2) ranging from 14 to 45.
Abstract translation: 本发明的气体分离部件包括膜，壁或中空纤维形式的多孔基材和通过等离子体聚合在基材的表面上形成的聚合物膜。 气体分离因子（O 2 / N 2）为2.3至3.9，相应的气体渗透率为12至0.16升/分钟。 m2 atm。 空气。 本发明的改性气体分离部件的气体分离系数（He / H 2）为14〜45，包括上述形式的多孔基材和通过等离子体聚合形成于基材表面的2个聚合物膜。
-
2.发明授权Method and apparatus for detecting the concentration of a component in a solution 失效用于检测溶液中组分浓度的方法和装置
公开(公告)号：US4481808A
公开(公告)日：1984-11-13
申请号：US234069
申请日：1981-02-12
Applicant: Jiro Sakata , Masana Hirai , Minoru Yamamoto
Inventor： Jiro Sakata , Masana Hirai , Minoru Yamamoto
CPC classification number: G01N7/10 , G01N33/2852
Abstract: Method and apparatus for determination of the concentration of a component in a solution, such as ethyl alcohol in gasohol, acetic acid in hexane-acetic acid solution, or the like. Such determination is made with ease and accuracy based on a rate of pressure change caused in a closed container due to mass transfer occurring between two liquids through porous material.
Abstract translation: 用于测定溶液中的组分浓度的方法和装置，如乙醇在汽油中，乙酸在己烷 - 乙酸溶液中等。 基于由于通过多孔材料在两种液体之间发生质量传递而在密闭容器中引起的压力变化的速率，这种确定是容易且准确的。
-
3.发明申请公开(公告)号：US20050214915A1
公开(公告)日：2005-09-29
申请号：US10507129
申请日：2003-03-11
Applicant: Satoshi Saito , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Takao Imaeda , Chikara Miyazaki , Kenro Tokuhiro
Inventor： Satoshi Saito , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Takao Imaeda , Chikara Miyazaki , Kenro Tokuhiro
CPC classification number: C12N9/0006 , C12P7/56 , C12Y101/01027
Abstract: The present invention provides a transformant into which has been incorporated DNA for coding a foreign protein having lactate dehydrogenase activity and provided with pyruvic acid substrate affinity that equals or exceeds the pyruvic acid substrate affinity of the pyruvate decarboxylase inherent in the host organism. Said transformant can stably mass-produce lactic acid inside a host organism having the pyruvate decarboxylase gene.
Abstract translation: 本发明提供了一种转化体，其中掺入了用于编码具有乳酸脱氢酶活性的外源蛋白质的DNA并且具有等于或超过固有在宿主生物体中的丙酮酸脱羧酶的丙酮酸底物亲和力的丙酮酸底物亲和力的DNA。 所述转化体可以在具有丙酮酸脱羧酶基因的宿主生物体内稳定地大量生产乳酸。
-
4.发明授权公开(公告)号：US08039238B2
公开(公告)日：2011-10-18
申请号：US10507129
申请日：2003-03-11
Applicant: Satoshi Saito , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Takao Imaeda , Chikara Miyazaki , Kenro Tokuhiro
Inventor： Satoshi Saito , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Takao Imaeda , Chikara Miyazaki , Kenro Tokuhiro
CPC classification number: C12N9/0006 , C12P7/56 , C12Y101/01027
Abstract: The present invention provides a transformant into which has been incorporated DNA for coding a foreign protein having lactate dehydrogenase activity and provided with pyruvic acid substrate affinity that equals or exceeds the pyruvic acid substrate affinity of the pyruvate decarboxylase inherent in the host organism. Said transformant can stably mass-produce lactic acid inside a host organism having the pyruvate decarboxylase gene.
Abstract translation: 本发明提供了一种转化体，其中掺入了用于编码具有乳酸脱氢酶活性的外源蛋白质的DNA并且具有等于或超过固有在宿主生物体中的丙酮酸脱羧酶的丙酮酸底物亲和力的丙酮酸底物亲和力的DNA。 所述转化体可以在具有丙酮酸脱羧酶基因的宿主生物体内稳定地大量生产乳酸。
-
公开(公告)号：US5702883A
公开(公告)日：1997-12-30
申请号：US326949
申请日：1994-10-21
Applicant: Takao Imaeda , Masana Hirai
Inventor： Takao Imaeda , Masana Hirai
CPC classification number: C07K14/47 , C12Q1/025 , C12Q1/6897
Abstract: A method for detecting or quantitating a mutagenic substance in a sample includes culturing a host microorganism transformed with a recombinant gene comprising an SOS gene and genes expressing luciferase activity and optionally genes expressing an enzyme which catalyzes the production of a substrate for luciferase, positioned downstream of the SOS gene, in a medium to which the sample is added; and measuring a luminescence generated by expression of the gene expressing luciferase activity. The method is sensitive, accurate and non-time consuming; and gene systems used for said method, i.e., a recombinant gene comprising an SOS gene expressed when a DNA is damaged and a gene expressing luciferase activity positioned downstream of the SOS gene, and a host microorganism transformed with said recombinant gene. Preferably the recombinant gene further comprises a gene expressing an enzyme which catalyses the production of a substrate for the luciferase in the down stream of the SOS gene.
Abstract translation: 用于检测或定量样品中的致突变物质的方法包括培养用包含SOS基因的重组基因和表达荧光素酶活性的基因转化的宿主微生物，以及任选地表达催化产生荧光素酶底物的酶的基因，所述基因位于下游 在添加样品的培养基中的SOS基因; 并测定通过表达荧光素酶活性的基因的表达产生的发光。 该方法灵敏，准确，无耗时; 以及用于所述方法的基因系统，即包含DNA受损时表达的SOS基因的重组基因和位于SOS基因下游的表达荧光素酶活性的基因以及用所述重组基因转化的宿主微生物。 优选地，重组基因还包含表达在SOS基因的下游中催化萤光素酶底物的产生的基因的基因。
-
Patent Agency Ranking6.发明授权Polypeptide possessing protein disulfide isomerase activity gene encoding the same and process for producing the same 失效具有编码相同蛋白质二硫键异构酶活性基因的多肽及其制备方法
公开(公告)号：US5700659A
公开(公告)日：1997-12-23
申请号：US464365
申请日：1995-06-05
Applicant: Yukio Yamada , Osamu Asami , Hidehiko Sugiyama , Chie Idekoba , Fumihiko Hoshino , Masana Hirai , Tsutomu Kajino , Takao Imaeda , Kiyoko Sarai
Inventor： Yukio Yamada , Osamu Asami , Hidehiko Sugiyama , Chie Idekoba , Fumihiko Hoshino , Masana Hirai , Tsutomu Kajino , Takao Imaeda , Kiyoko Sarai
CPC classification number: C12N9/90
Abstract: A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polyeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins. Further, a process which enables the polylpeptide possessing PDI activity to be efficiently produced using Humicola insolens or a transformant transformed with an expression vector containing the above-described gene is also provided.
Abstract translation: 提供了具有蛋白质二硫键异构酶（PDI）活性的高度热稳定的多肽，编码多肽的基因和产生多肽的方法。 具有PDI活性的多肽的特征在于：A）具有催化蛋白质中二硫键交换的能力，B）主要以核糖核酸酶A为底物，C）具有20〜70℃的合适的活性温度，D）为 在pH值为6〜9的条件下稳定，E）分子量约为60,000〜62,000。 由于与常规PDI相比，具有较高的热稳定性并且在更宽的二硫苏糖醇浓度范围内表现出稳定的活性，因此可以提供可有利地用于某些蛋白质的重折叠反应的新型酶活性蛋白质。 此外，还提供了使用Humicola insolens或用含有上述基因的表达载体转化的转化体能够有效地产生具有PDI活性的多肽的方法。
-
7.发明授权DNA encoding a protein having D-lactate dehydrogenase activity and uses thereof 有权编码具有D-乳酸脱氢酶活性的蛋白质的DNA及其用途公开(公告)号：US07964382B2
公开(公告)日：2011-06-21
申请号：US12324804
申请日：2008-11-26
Applicant: Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
Inventor： Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
IPC: C12N9/04 , C12N1/20 , C12N15/00 , C12P7/56 , C12P21/04 , C12Q1/00 , C12Q1/68 , C12Q1/32 , C07H21/04
CPC classification number: C12N9/0006 , C12P7/56 , C12P7/625
Abstract: This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).
Abstract translation: 本发明提供编码具有乳酸脱氢酶活性的蛋白质的多核苷酸和可用于生产D-乳酸的蛋白质。 该多核苷酸具有SEQ ID NO：1（a）所示的核苷酸序列，并且在严格条件下与包含SEQ ID NO：1所示的全部或部分核苷酸序列或其互补链的探针杂交，并编码 具有D-乳酸脱氢酶活性的蛋白质（b）。
-
8.发明申请Dna encoding a protein having d-lactate dehydrogenase activity and uses thereof 审中-公开Dna编码具有d-乳酸脱氢酶活性的蛋白质及其用途公开(公告)号：US20070105202A1
公开(公告)日：2007-05-10
申请号：US10557306
申请日：2004-05-21
Applicant: Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
Inventor： Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
CPC classification number: C12N9/0006 , C12P7/56 , C12P7/625
Abstract: This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).
Abstract translation: 本发明提供编码具有乳酸脱氢酶活性的蛋白质的多核苷酸和可用于生产D-乳酸的蛋白质。 该多核苷酸具有SEQ ID NO：1（a）所示的核苷酸序列，并且在严格条件下与包含SEQ ID NO：1所示的全部或部分核苷酸序列或其互补链的探针杂交，并编码 具有D-乳酸脱氢酶活性的蛋白质（b）。
-
公开(公告)号：US20050120394A1
公开(公告)日：2005-06-02
申请号：US10490046
申请日：2002-09-13
Applicant: Satoshi Saitoh , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Katsuhiko Kitamoto
Inventor： Satoshi Saitoh , Osamu Saotome , Noriko Yasutani , Yasuo Matsuo , Nobuhiro Ishida , Masana Hirai , Katsuhiko Kitamoto
IPC: C12N1/19 , C12N1/21 , C12N15/69 , C12N15/81 , A01K67/00 , A01H1/00 , C07H21/04 , C12N1/18 , C12N9/06 , C12N15/74
Abstract: The present invention relates to a method of expressing a gene by inserting the genome into a host organism using genetic engineering techniques. The present invention further relates to a novel promoter, a recombinant vector containing the promoter and a target gene, a transformant containing the recombinant vector, and a method of producing a useful gene product or useful substance using the transformant.
Abstract translation: 本发明涉及通过使用基因工程技术将基因组插入宿主生物体来表达基因的方法。 本发明还涉及一种新型启动子，含有启动子和靶基因的重组载体，含有重组载体的转化体，以及使用该转化体生产有用的基因产物或有用物质的方法。
-
10.发明申请DNA Encoding a Protein Having a D-Lactate Dehydrogenase Activity and Uses Thereof 有权编码具有D-乳酸脱氢酶活性的蛋白质的DNA及其用途公开(公告)号：US20090275095A1
公开(公告)日：2009-11-05
申请号：US12324804
申请日：2008-11-26
Applicant: Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
Inventor： Nobuhiro Ishida , Kenro Tokuhiro , Haruo Takahashi , Eiji Nagamori , Masana Hirai , Satoshi Saitoh , Tohru Ohnishi
CPC classification number: C12N9/0006 , C12P7/56 , C12P7/625
Abstract: This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).
Abstract translation: 本发明提供编码具有乳酸脱氢酶活性的蛋白质的多核苷酸和可用于生产D-乳酸的蛋白质。 该多核苷酸具有SEQ ID NO：1（a）所示的核苷酸序列，并且在严格条件下与包含SEQ ID NO：1所示的全部或部分核苷酸序列或其互补链的探针杂交，并编码 具有D-乳酸脱氢酶活性的蛋白质（b）。
-
-
-
-
-
-
-
-
-
Search Results
12
records
(took 0.204 seconds)
