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    FLUID CACHING
    4.
    发明申请
    FLUID CACHING 有权

    公开(公告)号：US20210154667A1

    公开(公告)日：2021-05-27

    申请号：US17165757

    申请日：2021-02-02

    申请人： Illumina, Inc. ,  Illumina Cambridge Limited

    发明人： Wesley A. Cox-Muranami ,  Darren Robert Segale ,  Oliver Jon Miller ,  Jennifer Olivia Foley ,  Tarun Kumar Khurana ,  Paul Crivelli ,  Gary Watts

    IPC分类号： B01L3/00 ,  G01N1/31

    摘要： An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.

    SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS
    7.
    发明申请
    SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS 有权

    公开(公告)号：US20220243269A1

    公开(公告)日：2022-08-04

    申请号：US17720187

    申请日：2022-04-13

    申请人： ILLUMINA, INC.

    发明人： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

    IPC分类号： C12Q1/6874 ,  C12Q1/6813

    摘要： Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

    IMMOBILIZATION IN FLOW CELLS
    8.
    发明申请
    IMMOBILIZATION IN FLOW CELLS 有权

    公开(公告)号：US20220195519A1

    公开(公告)日：2022-06-23

    申请号：US17600526

    申请日：2020-12-11

    申请人： ILLUMINA, INC.

    发明人： Jeffrey S. Fisher ,  Tarun Kumar Khurana ,  Mathieu Lessard-Viger ,  Clifford Lee Wang ,  Yir-Shyuan Wu

    IPC分类号： C12Q1/6874 ,  G01N1/40

    摘要： In an example, a target material is immobilized on two opposed sequencing surfaces of a flow cell using first and second fluids. The first fluid has a density less than a target material density and the second fluid has a density greater than the target material density; or the second fluid has a density less than the target material density and the first fluid has a density greater than the target material density. The first fluid (including the target material) is introduced into the flow cell, whereby at least some of the target material becomes immobilized by capture sites on one of the sequencing surfaces. The first fluid and non-immobilized target material are removed. The second fluid (including target material) is introduced into the flow cell, whereby at least some of the target material becomes immobilized by capture sites on another of the sequencing surfaces.

    Fluid caching
    9.
    发明授权
    Fluid caching 有权

    公开(公告)号：US11291998B2

    公开(公告)日：2022-04-05

    申请号：US17165757

    申请日：2021-02-02

    申请人： Illumina, Inc. ,  Illumina Cambridge Limited

    发明人： Wesley A. Cox-Muranami ,  Darren Robert Segale ,  Oliver Jon Miller ,  Jennifer Olivia Foley ,  Tarun Kumar Khurana ,  Paul Crivelli ,  Gary Watts

    IPC分类号： B01L3/00 ,  G01N1/31 ,  B01L9/00

    摘要： An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.

    Beads as Transposome Carriers
    10.
    发明公开
    Beads as Transposome Carriers 审中-公开

    公开(公告)号：US20230287476A1

    公开(公告)日：2023-09-14

    申请号：US18151128

    申请日：2023-01-06

    申请人： Illumina, Inc.

    发明人： Yir-Shyuan Wu ,  Tarun Kumar Khurana ,  Elisabet Rosàs-Canyelles ,  Fei Shen ,  Jeffrey Brodin ,  Lena Storms ,  Jeffrey S. Fisher

    IPC分类号： C12Q1/6806 ,  B82Y5/00 ,  C12Q1/6834

    CPC分类号： C12Q1/6806 ,  B82Y5/00 ,  C12Q1/6834

    摘要： Degradable polyester bead are described comprising a plurality of transposome complexes immobilized to the surface thereof, wherein each transposome complex comprises a transposase bound to a first polynucleotide and a second polynucleotide, wherein the first polynucleotide comprises a 3′ portion comprising a transposon end sequence and a tag, and the second polynucleotide comprises a 5′ portion that is complementary to and hybridized to the transposon end sequence, and wherein the polyester bead has a melting point of from 50° C. to 65° C. Flow cells and methods related to these polyester beads are described. Also described herein are compositions comprising a bead and at least one nanoparticle and methods of use of such compositions comprising transposome complexes immobilized to nanoparticles.