公开(公告)号：US20240209419A1

公开(公告)日：2024-06-27

申请号：US18539988

申请日：2023-12-14

申请人： Illumina, Inc.

发明人： Naneki McCallum ,  Fei Shen ,  Xiaoyu Ma ,  Weixian Xi ,  Olivia Ghazinejad ,  Jeffrey Brodin ,  Jeff Fisher

IPC分类号： C12Q1/6818 ,  C12Q1/6874

CPC分类号： C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6851

摘要： Methods for on-flow cell selective capture and enrichment of clustered beads, general capture strategies on bead mobility on flow cell surfaces, sorting clustered and unclustered beads, and flow cell reusability for bead immobilization onto flow cells.

公开(公告)号：US20240279733A1

公开(公告)日：2024-08-22

申请号：US18567989

申请日：2022-12-15

申请人： Illumina, Inc.

发明人： Fei Shen ,  Mathieu Lessard-Viger ,  Eric Brustad ,  Allison Meade ,  Esteban Armijo ,  Michael Howard ,  Jeffrey Fisher ,  Jonathan Boutell ,  Ramon Saracho ,  Olivia Ghazinejad ,  Seth McDonald ,  Lena Storms ,  Jeffrey Brodin

IPC分类号： C12Q1/6874

CPC分类号： C12Q1/6874

摘要： The present disclosure is directed to decoupling library capture (template seeding) from cluster generation to optimise both processes. This is achieved by introducing orthogonality between the seeding and clustering primer.

公开(公告)号：US20240271126A1

公开(公告)日：2024-08-15

申请号：US18562095

申请日：2022-05-26

申请人： ILLUMINA, INC.

发明人： Niall Anthony Gormley ,  Carlo Randise-Hinchliff ,  Jeffrey Brodin ,  Esther Musgrave-Brown ,  Sarah E. Shultzaberger ,  Andrew Slatter ,  Jeffrey S. Fisher

IPC分类号： C12N15/10 ,  C12N15/11 ,  C12Q1/48

CPC分类号： C12N15/1096 ,  C12N15/11 ,  C12Q1/485

摘要： Nucleic acid techniques are disclosed. Embodiments include modified nucleotides with oligonucleotide adapters that are coupled via cleavable linkers. Incorporation of the modified nucleotide at a 3′ end of a nucleic acid permits end-adapterization via ligation of a free 5′ end of the oligonucleotide adapter to a 3′ reactive group of the modified nucleotide and cleavage at the cleavable linker to liberate a free 3′ end.

公开(公告)号：US20230287476A1

公开(公告)日：2023-09-14

申请号：US18151128

申请日：2023-01-06

申请人： Illumina, Inc.

发明人： Yir-Shyuan Wu ,  Tarun Kumar Khurana ,  Elisabet Rosàs-Canyelles ,  Fei Shen ,  Jeffrey Brodin ,  Lena Storms ,  Jeffrey S. Fisher

IPC分类号： C12Q1/6806 ,  B82Y5/00 ,  C12Q1/6834

CPC分类号： C12Q1/6806 ,  B82Y5/00 ,  C12Q1/6834

摘要： Degradable polyester bead are described comprising a plurality of transposome complexes immobilized to the surface thereof, wherein each transposome complex comprises a transposase bound to a first polynucleotide and a second polynucleotide, wherein the first polynucleotide comprises a 3′ portion comprising a transposon end sequence and a tag, and the second polynucleotide comprises a 5′ portion that is complementary to and hybridized to the transposon end sequence, and wherein the polyester bead has a melting point of from 50° C. to 65° C. Flow cells and methods related to these polyester beads are described. Also described herein are compositions comprising a bead and at least one nanoparticle and methods of use of such compositions comprising transposome complexes immobilized to nanoparticles.