公开(公告)号：US20220106588A1

公开(公告)日：2022-04-07

申请号：US17451771

申请日：2021-10-21

申请人： Illumina, Inc.

发明人： Yir-Shyuan Wu ,  Filiz Gorpe-Yasar ,  Tarun Kumar Khurana ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6834

摘要： Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.

公开(公告)号：US11352668B2

公开(公告)日：2022-06-07

申请号：US16626115

申请日：2018-07-31

申请人： ILLUMINA, INC.

发明人： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC分类号： C12Q1/6874 ,  C12Q1/6813

摘要： Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US20200216895A1

公开(公告)日：2020-07-09

申请号：US16626115

申请日：2018-07-31

申请人： ILLUMINA, INC.

发明人： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC分类号： C12Q1/6874 ,  C12Q1/6813

摘要： Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US11649498B2

公开(公告)日：2023-05-16

申请号：US17720187

申请日：2022-04-13

申请人： ILLUMINA, INC.

发明人： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC分类号： C12Q1/6874 ,  C12Q1/6813

CPC分类号： C12Q1/6874 ,  C12Q1/6813 ,  C12Q2565/514 ,  C12Q2600/156

摘要： Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US20220243269A1

公开(公告)日：2022-08-04

申请号：US17720187

申请日：2022-04-13

申请人： ILLUMINA, INC.

发明人： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC分类号： C12Q1/6874 ,  C12Q1/6813

摘要： Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US20230220462A1

公开(公告)日：2023-07-13

申请号：US18180622

申请日：2023-03-08

申请人： ILLUMINA, INC.

发明人： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC分类号： C12Q1/6874 ,  C12Q1/6813

CPC分类号： C12Q1/6874 ,  C12Q1/6813 ,  C12Q2600/156 ,  C12Q2565/514

摘要： Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US11180752B2

公开(公告)日：2021-11-23

申请号：US16272870

申请日：2019-02-11

申请人： Illumina, Inc.

发明人： Yir-Shyuan Wu ,  Filiz Gorpe-Yasar ,  Tarun Kumar Khurana ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC分类号： C12N15/10 ,  B01L3/00 ,  C12Q1/6834 ,  C12Q1/6806 ,  B01L7/00

摘要： Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.

公开(公告)号：US20190249171A1

公开(公告)日：2019-08-15

申请号：US16272870

申请日：2019-02-11

申请人： Illumina, Inc.

发明人： Yir-Shyuan Wu ,  Filiz Gorpe-Yasar ,  Tarun Kumar Khurana ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12N15/1068 ,  B01L3/508 ,  B01L7/52 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/123 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2527/156 ,  C12Q2531/119 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/629

摘要： Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.