公开(公告)号：US12186746B2

公开(公告)日：2025-01-07

申请号：US17255819

申请日：2020-02-07

Applicant: ILLUMINA, INC.

Inventor： Aathavan Karunakaran ,  Arnaud Rival ,  Ali Agah ,  Fabien Abeille ,  Steven Barnard ,  Craig Ciesla ,  Murali Venkatesan ,  Pargol Gheissari ,  Jennifer Wang ,  Dietrich Dehlinger

IPC: B01L3/00

Abstract: A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell having an inlet port, an outlet port, and a flow channel extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device.

公开(公告)号：US20200238276A1

公开(公告)日：2020-07-30

申请号：US16750930

申请日：2020-01-23

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Arnaud Rival ,  Lewis J. Kraft ,  Steven Barnard ,  M. Shane Bowen ,  Xi-Jun Chen ,  Yir-Shyuan Wu ,  Jeffrey S. Fisher ,  Dajun Yuan

IPC: B01L3/00 ,  C12N15/10

Abstract: An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.

公开(公告)号：USRE49884E1

公开(公告)日：2024-03-26

申请号：US17214093

申请日：2021-06-08

Applicant: ILLUMINA, INC.

Inventor： Wenyi Feng ,  Jason Bryant ,  Steven Barnard ,  Maria Candelaria Rogert Bacigalupo

IPC: G01N21/64 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/03 ,  G01N21/05 ,  G02B21/00

CPC classification number: G01N21/6458 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/05 ,  G02B21/0024 ,  G01N2021/0346 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B2207/113

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：US20220331792A1

公开(公告)日：2022-10-20

申请号：US17729814

申请日：2022-04-26

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Arnaud Rival ,  Lewis J. Kraft ,  Steven Barnard ,  M. Shane Bowen ,  Xi-Jun Chen ,  Yir-Shyuan Wu ,  Jeffrey S. Fisher ,  Dajun Yuan

IPC: B01L3/00 ,  C12N15/10

Abstract: An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.

公开(公告)号：USRE48561E1

公开(公告)日：2021-05-18

申请号：US16000720

申请日：2018-06-05

Applicant: ILLUMINA, INC.

Inventor： Wenyi Feng ,  Jason Bryant ,  Steven Barnard ,  Maria Candelaria Rogert Bacigalupo

IPC: G01N21/05 ,  G01N21/64 ,  C12Q1/6825 ,  C12Q1/6874 ,  G02B21/00 ,  C12Q1/6869 ,  G01N21/03

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

公开(公告)号：US20220410146A1

公开(公告)日：2022-12-29

申请号：US17898155

申请日：2022-08-29

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Arnaud Rival ,  Lewis J. Kraft ,  Steven Barnard ,  M. Shane Bowen ,  Xi-Jun Chen ,  Yir-Shyuan Wu ,  Jeffrey S. Fisher ,  Dajun Yuan

IPC: B01L3/00 ,  C12N15/10

Abstract: An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.

公开(公告)号：US20200157618A1

公开(公告)日：2020-05-21

申请号：US16626425

申请日：2019-02-13

Applicant: Illumina, Inc.

Inventor： Jeffrey Mandell ,  Steven Barnard ,  John Moon ,  Maria Candelaria Rogert Bacigalupo

IPC: C12Q1/6869 ,  C07H19/06 ,  B01L3/00

Abstract: A labeled nucleotide includes a nucleotide, a linking molecule attached to a phosphate group of the nucleotide, and a redox-active charge tag attached to the linking molecule. The redox-active charge tag is to be oxidized or reduced by an electrically conductive channel when maintained in proximity of a sensing zone of the electrically conductive channel.

公开(公告)号：US11819843B2

公开(公告)日：2023-11-21

申请号：US17729814

申请日：2022-04-26

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Arnaud Rival ,  Lewis J. Kraft ,  Steven Barnard ,  M. Shane Bowen ,  Xi-Jun Chen ,  Yir-Shyuan Wu ,  Jeffrey S. Fisher ,  Dajun Yuan

IPC: B01L3/00 ,  C12N15/10 ,  C08K5/54 ,  C08L37/00

CPC classification number: B01L3/5027 ,  B01L3/5085 ,  C12N15/1065 ,  B01L3/5088 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2300/165 ,  C08K5/5406 ,  C08L37/00

Abstract: An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.

公开(公告)号：US11542550B2

公开(公告)日：2023-01-03

申请号：US16626425

申请日：2019-02-13

Applicant: Illumina, Inc.

Inventor： Jeffrey Mandell ,  Steven Barnard ,  John Moon ,  Maria Candelaria Rogert Bacigalupo

IPC: C12Q1/68 ,  C07H19/06 ,  C12Q1/6869 ,  B01L3/00

Abstract: A labeled nucleotide includes a nucleotide, a linking molecule attached to a phosphate group of the nucleotide, and a redox-active charge tag attached to the linking molecule. The redox-active charge tag is to be oxidized or reduced by an electrically conductive channel when maintained in proximity of a sensing zone of the electrically conductive channel.

公开(公告)号：US20250108375A1

公开(公告)日：2025-04-03

申请号：US18979010

申请日：2024-12-12

Applicant: ILLUMINA, INC.

Inventor： Aathavan Karunakaran ,  Arnaud Rival ,  Ali Agah ,  Fabien Abeille ,  Steven Barnard ,  Craig Ciesla ,  Murali Venkatesan ,  Pargol Gheissari ,  Jennifer Wang ,  Dietrich Dehlinger

IPC: B01L3/00

Abstract: A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell having an inlet port, an outlet port, and a flow channel extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device.