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    Ribozymes with broad tRNA aminoacylation activity
    1.
    发明授权
    Ribozymes with broad tRNA aminoacylation activity 有权
    具有广泛tRNA氨基酰化活性的核酶

    公开(公告)号：US07622248B2

    公开(公告)日：2009-11-24

    申请号：US10369036

    申请日：2003-02-18

    申请人： Hiroaki Suga ,  Hiroshi Murakami ,  Hirohide Saito

    发明人： Hiroaki Suga ,  Hiroshi Murakami ,  Hirohide Saito

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12P21/00 ,  C12N15/113 ,  C12N2310/12

    摘要： The present invention provides catalytic RNA molecules having cis or trans aminoacylation activity. The catalytic RNA molecules having cis aminoacylation activity comprise a catalytic domain and an aminoacylation domain. The catalytic RNA molecules having trans aminoacylation activity only have the catalytic domain. A method is provided for constructing and screening of these molecules. These molecules are suitable for aminoacylating with specific amino acids.

    摘要翻译： 本发明提供具有顺式或反式氨基酰化活性的催化RNA分子。 具有顺式氨基酰化活性的催化RNA分子包含催化结构域和氨基酰化结构域。 具有反氨基酰化活性的催化RNA分子仅具有催化结构域。 提供了构建和筛选这些分子的方法。 这些分子适合用特定氨基酸进行氨基酰化。

    Catalytic RNAs with aminoacylation activity
    2.
    发明授权
    Catalytic RNAs with aminoacylation activity 有权
    具有氨基酰化活性的催化RNA

    公开(公告)号：US07001723B1

    公开(公告)日：2006-02-21

    申请号：US09721414

    申请日：2000-11-22

    申请人： Hiroaki Suga ,  Dimitrios Kourouklis ,  Hirohide Saito ,  Nick Lee ,  Neil Bonzagni

    发明人： Hiroaki Suga ,  Dimitrios Kourouklis ,  Hirohide Saito ,  Nick Lee ,  Neil Bonzagni

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12N15/113 ,  C12N2310/12

    摘要： The present invention provides catalytic RNA molecules having cis or trans aminoacylation activity. The catalytic RNA molecules having cis aminoacylation activity comprise a catalytic domain and an aminoacylation domain. The catalytic RNA molecules having trans aminoacylation activity only have the catalytic domain. A method is provided for constructing and screening of these molecules. These molecules are suitable for aminoacylating tRNA-like molecules with specific amino acids.

    摘要翻译： 本发明提供具有顺式或反式氨基酰化活性的催化RNA分子。 具有顺式氨基酰化活性的催化RNA分子包含催化结构域和氨基酰化结构域。 具有反氨基酰化活性的催化RNA分子仅具有催化结构域。 提供了构建和筛选这些分子的方法。 这些分子适用于具有特定氨基酸的氨基酰化tRNA样分子。

    NOVEL ARTIFICIAL TRANSLATION/SYNTHESIS SYSTEM
    3.
    发明申请
    NOVEL ARTIFICIAL TRANSLATION/SYNTHESIS SYSTEM 有权
    新人工翻译/合成系统

    公开(公告)号：US20130217599A1

    公开(公告)日：2013-08-22

    申请号：US13816911

    申请日：2011-08-26

    申请人： Hiroaki Suga ,  Hiroshi Murakami ,  Yuki Goto

    发明人： Hiroaki Suga ,  Hiroshi Murakami ,  Yuki Goto

    IPC分类号： C12P21/00

    CPC分类号： C12P21/00 ,  C12N15/67 ,  C12P21/02

    摘要： A new artificial translation-synthesis system of adding tRNAs binding special amino acids to the in vitro translation system and synthesizing peptides with special amino acids incorporated thereto according to a dual genetic code table and an artificial codon box division.

    摘要翻译： 一种新的人工翻译 - 合成系统，其将特异性氨基酸结合到体外翻译系统中并根据双重遗传密码表和人造密码子分组合成具有特异氨基酸的肽。

    METHODS FOR RIBOSOMAL SYNTHESIS OF POLYPEPTIDES CONTAINING UNNATURAL N-TERMINAL GROUPS AND APPLICATIONS THEREOF
    4.
    发明申请
    METHODS FOR RIBOSOMAL SYNTHESIS OF POLYPEPTIDES CONTAINING UNNATURAL N-TERMINAL GROUPS AND APPLICATIONS THEREOF 有权
    含有未知N末端组的多肽的RIBOSOMAL合成方法及其应用

    公开(公告)号：US20110275119A1

    公开(公告)日：2011-11-10

    申请号：US12515074

    申请日：2007-11-13

    申请人： Hiroaki Suga ,  Hiroshi Murakami ,  Yuki Goto ,  Atsushi Ohta

    发明人： Hiroaki Suga ,  Hiroshi Murakami ,  Yuki Goto ,  Atsushi Ohta

    IPC分类号： C12P21/00 ,  C12N9/10

    CPC分类号： C12N15/67 ,  C12P21/02

    摘要： The present invention aims to synthesize a polypeptide having an unnatural structure at the N-terminus via a biosynthetic process by translation of amino acid sequence information encoded by a nucleic acid. A polypeptide having any amino acid at the N-terminus is synthesized by using an ARS ribozyme that catalyzes the acylation of tRNA with any amino acid to attach any amino acid to an initiator tRNA, thereby initiating a translation with the initiator tRNA.

    摘要翻译： 本发明旨在通过生物合成方法通过翻译由核酸编码的氨基酸序列信息在N末端合成具有非天然结构的多肽。 通过使用催化tRNA与任何氨基酸酰化以将任何氨基酸连接至引发剂tRNA的ARS核酶合成在N末端具有任何氨基酸的多肽，从而开始与引发剂tRNA的翻译。

    PROCESS FOR SYNTHESIZING CYCLIC PEPTIDE COMPOUND
    5.
    发明申请
    PROCESS FOR SYNTHESIZING CYCLIC PEPTIDE COMPOUND 有权
    合成环化合物的方法

    公开(公告)号：US20100168380A1

    公开(公告)日：2010-07-01

    申请号：US12593221

    申请日：2008-03-26

    申请人： Hiroaki Suga ,  Hiroshi Murakami ,  Yuki Goto ,  Yusuke Yamagishi ,  Hiroshi Ashigai ,  Yusuke Sako

    发明人： Hiroaki Suga ,  Hiroshi Murakami ,  Yuki Goto ,  Yusuke Yamagishi ,  Hiroshi Ashigai ,  Yusuke Sako

    IPC分类号： C07K1/00 ,  C12P21/00

    CPC分类号： C07K7/56 ,  C07K7/06 ,  C07K7/08 ,  C12N15/67 ,  C12P21/02

    摘要： The objective is to provide a novel process for synthesizing a cyclic peptide compound. It is also an objective to provide a novel cyclic peptide compound. The novel process for synthesizing a cyclic peptide compound comprises the steps of: (1) translationally synthesizing a non-cyclic peptide compound having in a molecule a functional group 1 and a functional group 2, which are a pair of functional groups capable of reacting to form a bond, and (2) cyclizing the non-cyclic peptide compound by the reaction of the functional groups 1 and 2 to form a bond. The novel cyclic peptide compound can be synthesized by the process.

    摘要翻译： 目的是提供一种合成环肽化合物的新方法。 提供新的环肽化合物也是目的。 用于合成环肽化合物的新方法包括以下步骤：（1）在分子中转化合成具有官能团1和官能团2的能够对 形成键，和（2）通过官能团1和2的反应使非环肽化合物环化以形成键。 可以通过该方法合成新的环肽化合物。

    Methods for ribosomal synthesis of polypeptides containing unnatural N-terminal groups and applications thereof
    6.
    发明授权
    Methods for ribosomal synthesis of polypeptides containing unnatural N-terminal groups and applications thereof 有权
    含有非天然N端基的多肽的核糖体合成方法及其应用

    公开(公告)号：US08557542B2

    公开(公告)日：2013-10-15

    申请号：US12515074

    申请日：2007-11-13

    申请人： Hiroaki Suga ,  Hiroshi Murakami ,  Yuki Goto ,  Atsushi Ohta

    发明人： Hiroaki Suga ,  Hiroshi Murakami ,  Yuki Goto ,  Atsushi Ohta

    IPC分类号： C12P21/06

    CPC分类号： C12N15/67 ,  C12P21/02

    摘要： The present invention aims to synthesize a polypeptide having an unnatural structure at the N-terminus via a biosynthetic process by translation of amino acid sequence information encoded by a nucleic acid. A polypeptide having any amino acid at the N-terminus is synthesized by using an ARS ribozyme that catalyzes the acylation of tRNA with any amino acid to attach any amino acid to an initiator tRNA, thereby initiating a translation with the initiator tRNA.

    摘要翻译： 本发明旨在通过生物合成方法通过翻译由核酸编码的氨基酸序列信息在N末端合成具有非天然结构的多肽。 通过使用催化tRNA与任何氨基酸酰化以将任何氨基酸连接至引发剂tRNA的ARS核酶合成在N末端具有任何氨基酸的多肽，从而开始与引发剂tRNA的翻译。

    Process for synthesizing cyclic peptide compound
    8.
    发明授权
    Process for synthesizing cyclic peptide compound 有权
    环肽化合物的合成方法

    公开(公告)号：US09090668B2

    公开(公告)日：2015-07-28

    申请号：US12593221

    申请日：2008-03-26

    申请人： Hiroaki Suga ,  Hiroshi Murakami ,  Yuki Goto ,  Yusuke Yamagishi ,  Hiroshi Ashigai ,  Yusuke Sako

    发明人： Hiroaki Suga ,  Hiroshi Murakami ,  Yuki Goto ,  Yusuke Yamagishi ,  Hiroshi Ashigai ,  Yusuke Sako

    IPC分类号： C07K7/56 ,  C07K7/06 ,  C07K7/08 ,  C12N15/67 ,  C12P21/02

    CPC分类号： C07K7/56 ,  C07K7/06 ,  C07K7/08 ,  C12N15/67 ,  C12P21/02

    摘要： The objective is to provide a novel process for synthesizing a cyclic peptide compound. It is also an objective to provide a novel cyclic peptide compound. The novel process for synthesizing a cyclic peptide compound comprises the steps of: (1) translationally synthesizing a non-cyclic peptide compound having in a molecule a functional group 1 and a functional group 2, which are a pair of functional groups capable of reacting to form a bond, and (2) cyclizing the non-cyclic peptide compound by the reaction of the functional groups 1 and 2 to form a bond. The novel cyclic peptide compound can be synthesized by the process.

    摘要翻译： 目的是提供一种合成环肽化合物的新方法。 提供新的环肽化合物也是目的。 用于合成环肽化合物的新方法包括以下步骤：（1）在分子中转化合成具有官能团1和官能团2的非环状肽化合物，所述官能团是能够使 形成键，和（2）通过官能团1和2的反应使非环肽化合物环化以形成键。 可以通过该方法合成新的环肽化合物。

    Versatile acylation catalytic RNAs and uses thereof
    9.
    发明授权
    Versatile acylation catalytic RNAs and uses thereof 有权
    多功能酰化催化RNA及其用途

    公开(公告)号：US08188260B2

    公开(公告)日：2012-05-29

    申请号：US12086125

    申请日：2006-12-05

    申请人： Hiroaki Suga ,  Hiroshi Murakami

    发明人： Hiroaki Suga ,  Hiroshi Murakami

    IPC分类号： C07H21/04 ,  C07H21/02 ,  C12N15/85

    CPC分类号： C12N15/67 ,  C12N9/93 ,  C12P19/34

    摘要： An object of the present invention is to provide novel ribozyme systems capable of catalyzing tRNA acetylation using various carboxylic acids as acyl donors and uses thereof. Disclosed is a ribozyme catalyzing tRNA acetylation having a structure consisting of the RNA sequence represented by: (formula 1) P1-Z1Z2Z3Z4(N1)1(N1)2 . . . (N1)p-P2-(N2)1(N2)2 . . . (N2)q Y1Y2Y3-(N3)1(N3)2-N4GGN wherein (N1)1-(N1)p each independently represent any monoribonucleotide of U, C, A, and G; p represents 3 or 4; (N2)1-(N2)q each independently represent any monoribonucleotide of U, C, A, and G; q represents 5 or 6; (N3)1-(N3)2 each independently represent any monoribonucleotide of U, C, A, and G; N4 represents any monoribonucleotide of U, C, A, and G; Z1-Z4 each independently represent C or G; Y1-Y3 each independently represent C or G; N represents a monoribonucleotide complementary to A or G; and P1 and P2 represent a domain consisting of any RNA sequence capable of having a stem-loop structure.

    摘要翻译： 本发明的目的是提供能够使用各种羧酸作为酰基供体催化tRNA乙酰化的新型核酶系统及其用途。 公开了一种催化tRNA乙酰化的核酶，其结构由以下所示的RNA序列组成：（式1）P1-Z1Z2Z3Z4（N1）1（N1）2。 。 。 （N1）p-P2-（N2）1（N2）2。 。 。 （N 2）q Y1Y2Y3-（N3）1（N3）2-N4GGN其中（N1）1-（N1）p各自独立地表示U，C，A和G的任何单核糖核苷酸; p表示3或4; （N 2）1-（N 2）q各自独立地表示U，C，A和G的任何单核糖核苷酸; q表示5或6; （N3）1-（N3）2各自独立地表示U，C，A和G的任何单核糖核苷酸; N4代表U，C，A和G的任何单核糖核苷酸; Z1-Z4各自独立地表示C或G; Y1-Y3各自独立地表示C或G; N表示与A或G互补的单核糖核苷酸; P1和P2表示由能够具有茎 - 环结构的任何RNA序列组成的结构域。

    Versatile tRNA Acylation Catalytic RNAs and Uses Thereof
    10.
    发明申请
    Versatile tRNA Acylation Catalytic RNAs and Uses Thereof 有权
    多功能tRNA酰化催化RNA及其用途

    公开(公告)号：US20090281280A1

    公开(公告)日：2009-11-12

    申请号：US12086125

    申请日：2006-12-05

    申请人： Hiroaki Suga ,  Hiroshi Murakami

    发明人： Hiroaki Suga ,  Hiroshi Murakami

    IPC分类号： C07K1/107 ,  C07H21/02 ,  C07H21/04 ,  C07D209/20

    CPC分类号： C12N15/67 ,  C12N9/93 ,  C12P19/34

    摘要： An object of the present invention is to provide novel ribozyme systems capable of catalyzing tRNA acylation using various carboxylic acids as acyl donors and uses thereof.Disclosed is a ribozyme catalyzing tRNA acylation having a structure consisting of the RNA sequence represented by (formula 1): P1-Z1Z2Z3Z4(N1)1(N1)2 . . . (N1)p—P2-(N2)1(N2)2 . . . (N2)qY1Y2Y3(N3)1(N3)2N4GGN wherein (N1)1-(N1)p each independently represent any monoribonucleotide of U, C, A and G; p represents 3 or 4; (N2)1-(N2)q each independently represent any monoribonucleotide of U, C, A and G; q represents 5 or 6; (N3)1-(N3)2 each independently represent any monoribonucleotide of U, C, A and G; N4 represents any monoribonucleotide of U, C, A and G; Z1-Z4 each independently represent C or G; Y1-Y3 each independently represent C or G; N represents a monoribonucleotide complementary to A or G; and P1 and P2 represent a domain consisting of any RNA sequence capable of having a stem-loop structure.

    摘要翻译： 本发明的目的是提供能够使用各种羧酸作为酰基供体催化tRNA酰化的新型核酶体系及其用途。 公开了催化tRNA酰化的核酶，其具有由（式1）表示的RNA序列组成的结构：P1-Z1Z2Z3Z4（N1）1（N1）2。 。 。 （N1）p-P2-（N2）1（N2）2。 。 。 （N2）qY1Y2Y3（N3）1（N3）2N4GGN其中（N1）1-（N1）p各自独立地表示U，C，A和G的任何单核糖核苷酸; p表示3或4; （N2）1-（N2）q各自独立地表示U，C，A和G的任何单核糖核苷酸; q表示5或6; （N3）1-（N3）2各自独立地表示U，C，A和G的任何单核糖核苷酸; N4代表U，C，A和G的任何单核糖核苷酸; Z1-Z4各自独立地表示C或G; Y1-Y3各自独立地表示C或G; N表示与A或G互补的单核糖核苷酸; P1和P2表示由能够具有茎 - 环结构的任何RNA序列组成的结构域。