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公开(公告)号：US20200283732A1
公开(公告)日：2020-09-10
申请号：US16825213
申请日：2020-03-20
申请人： EMULATE INC.
摘要： An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.
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公开(公告)号：US20240309331A1
公开(公告)日：2024-09-19
申请号：US18591819
申请日：2024-02-29
申请人： Emulate, Inc.
发明人： S. Jordan Kerns , Catherine Karalis , Janna Nawroth , Remi Villenave , Jenifer Obrigewitch , Doris Roth , Michael Salmon , Athanasia Apostolou , David Conegliano
CPC分类号： C12N5/0679 , C12M23/16 , C12M35/04 , C12M35/08 , C12N1/20 , C12N5/0688 , C12Q1/04 , C12N2500/02 , C12N2502/70 , C12N2506/23 , C12N2537/00
摘要： The present invention relates to a combination of microbes, cell culture systems and microfluidic fluidic systems for use in providing a human Intestine On-Chip with optimal intestinal motility. More specifically, in some embodiments, a microfluidic chip containing intestinal epithelial cells co-cultured with intestinal endothelial cells in the presence of bacteria, such as probiotic bacteria, may find use in providing an Intestine-On-Chip for testing intestinal motility function. In some embodiments, an Intestine On-Chip may be used for identifying (testing) therapeutic compounds continuing probiotic microbes or compounds for inducing intestinal motility for use in treating gastrointestinal disorders or diseases related to intestinal function.
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公开(公告)号：US11566231B2
公开(公告)日：2023-01-31
申请号：US16825213
申请日：2020-03-20
申请人： EMULATE, INC.
摘要： An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.
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公开(公告)号：US20210054323A1
公开(公告)日：2021-02-25
申请号：US17017029
申请日：2020-09-10
申请人： EMULATE, Inc.
摘要： The present invention relates to a combination of cell culture systems and microfluidic fluidic systems for use in providing a human innervated Intestine On-Chip. More specifically, in some embodiments, a microfluidic chip containing intestinal epithelial cells co-cultured with intestinal endothelial cells or intestinal muscle cells, or both, in the presence of induced neural crest cells may find use in providing an innervated Intestine-On-Chip. In some embodiments, an innervated Intestine On-Chip may be used for identifying (testing) therapeutic compounds for use in treating gastrointestinal disorders or diseases.
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公开(公告)号：US20200224136A1
公开(公告)日：2020-07-16
申请号：US16742497
申请日：2020-01-14
申请人： EMULATE, INC.
摘要： The present invention relates to fluidic systems for use in providing biomarkers for human Intestine On-Chip. More specifically, in some embodiments, a microfluidic chip containing intestinal epithelial cells co-cultured with intestinal endothelial cells in the presence of stretch and flow are used for identifying differentially expressed genes as biomarkers, e.g. for specific types of drug testing for use in treating gastrointestinal disorders or diseases related to intestinal function.
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公开(公告)号：US20240301358A1
公开(公告)日：2024-09-12
申请号：US18602727
申请日：2024-03-12
申请人： EMULATE, Inc.
CPC分类号： C12N5/0679 , B01L3/502715 , B01L3/502761 , C12M23/16 , C12M23/26 , C12M25/02 , C12N5/069 , G01N1/30 , G01N33/5047 , G01N33/5064 , B01L2200/16 , B01L2300/123 , B01L2300/16 , C12N2500/00 , C12N2501/052 , C12N2501/2301 , C12N2501/2306 , C12N2501/25
摘要： An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.
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公开(公告)号：US12018284B2
公开(公告)日：2024-06-25
申请号：US17984023
申请日：2022-11-09
申请人： EMULATE, INC.
CPC分类号： C12N5/0679 , B01L3/502715 , B01L3/502761 , C12M23/16 , C12M23/26 , C12M25/02 , C12N5/069 , G01N1/30 , G01N33/5047 , G01N33/5064 , B01L2200/16 , B01L2300/123 , B01L2300/16 , C12N2500/00 , C12N2501/052 , C12N2501/2301 , C12N2501/2306 , C12N2501/25
摘要： An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.
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公开(公告)号：US20230151333A1
公开(公告)日：2023-05-18
申请号：US17984023
申请日：2022-11-09
申请人： EMULATE, INC.
CPC分类号： C12N5/0679 , C12M23/16 , C12M25/02 , G01N1/30
摘要： An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.
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公开(公告)号：US20210115406A1
公开(公告)日：2021-04-22
申请号：US17088828
申请日：2020-11-04
申请人： EMULATE, INC.
发明人： S. Jordan Kerns , Catherine Karalis , Janna Nawroth , Remi Villenave , Jenifer Obrigewitch , Doris Roth , Michael Salmon , Athanasia Apostolou , David Conegliano
摘要： The present invention relates to a combination of microbes, cell culture systems and microfluidic fluidic systems for use in providing a human Intestine On-Chip with optimal intestinal motility. More specifically, in some embodiments, a microfluidic chip containing intestinal epithelial cells co-cultured with intestinal endothelial cells in the presence of bacteria, such as probiotic bacteria, may find use in providing an Intestine-On-Chip for testing intestinal motility function. In some embodiments, an Intestine On-Chip may be used for identifying (testing) therapeutic compounds continuing probiotic microbes or compounds for inducing intestinal motility for use in treating gastrointestinal disorders or diseases related to intestinal function.
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