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公开(公告)号：US10851141B2
公开(公告)日：2020-12-01
申请号：US16311301
申请日：2017-06-19
Applicant: DKK-TOA Corporation
Inventor： Kenichi Noda , Satoshi Yawata , Ai Shimomura
Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.
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2.发明申请公开(公告)号：US20190352350A1
公开(公告)日：2019-11-21
申请号：US16311301
申请日：2017-06-19
Applicant: DKK-TOA Corporation
Inventor： Kenichi Noda , Satoshi Yawata , Ai Shimomura
IPC: C07K14/435 , C12P21/02 , C12N9/02 , C12N15/63
Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.
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公开(公告)号：US20150225768A1
公开(公告)日：2015-08-13
申请号：US14429784
申请日：2013-09-19
Applicant: DKK-TOA CORPORATION , HIROSHIMA UNIVERSITY
Inventor： Satoshi Yawata , Hiromitsu Hachiya , Akio Kuroda , Kenichi Noda
CPC classification number: C12Q1/66 , C12Q1/008 , G01N21/76 , G01N33/579 , G01N2400/24 , G01N2400/50
Abstract: A quantification method includes a calibration curve preparing step to measure a standard solution, which has been prepared by adding sodium ions so that a sodium ion content of the standard solution is equaled to a sodium ion content of a sample to be measured with a method employing a reaction that activates a limulus reagent and/or a biochemical luminescent reaction caused by ATP, luciferin, and luciferase, and to prepare a calibration curve that represents a relation between a measurement value and an amount of a component to be measured; a sample measuring step to measure the sample to be measured with a method being the same as that used in the calibration curve preparing step; and a quantifying step to find, by using the calibration curve, an amount of the component to be measured in the sample to be measured from a measurement value in the sample measuring step.
Abstract translation: 定量方法包括：通过添加钠离子制备标准溶液的标准溶液的校准曲线制备步骤，使标准溶液的钠离子含量与待测样品的钠离子含量相等， 激活鲎试剂和/或由ATP，荧光素和荧光素酶引起的生化发光反应的反应，并制备表示测量值与待测组分量之间的关系的校准曲线; 样品测量步骤，用与校准曲线制备步骤中使用的方法相同的方法测量待测样品; 以及量化步骤，通过使用校准曲线，从样品测量步骤中的测量值来求出待测样品中待测量的成分的量。
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