公开(公告)号：US20150225768A1

公开(公告)日：2015-08-13

申请号：US14429784

申请日：2013-09-19

Applicant: DKK-TOA CORPORATION ,  HIROSHIMA UNIVERSITY

Inventor： Satoshi Yawata ,  Hiromitsu Hachiya ,  Akio Kuroda ,  Kenichi Noda

IPC: C12Q1/66 ,  C12Q1/00

CPC classification number: C12Q1/66 ,  C12Q1/008 ,  G01N21/76 ,  G01N33/579 ,  G01N2400/24 ,  G01N2400/50

Abstract: A quantification method includes a calibration curve preparing step to measure a standard solution, which has been prepared by adding sodium ions so that a sodium ion content of the standard solution is equaled to a sodium ion content of a sample to be measured with a method employing a reaction that activates a limulus reagent and/or a biochemical luminescent reaction caused by ATP, luciferin, and luciferase, and to prepare a calibration curve that represents a relation between a measurement value and an amount of a component to be measured; a sample measuring step to measure the sample to be measured with a method being the same as that used in the calibration curve preparing step; and a quantifying step to find, by using the calibration curve, an amount of the component to be measured in the sample to be measured from a measurement value in the sample measuring step.

Abstract translation: 定量方法包括：通过添加钠离子制备标准溶液的标准溶液的校准曲线制备步骤，使标准溶液的钠离子含量与待测样品的钠离子含量相等， 激活鲎试剂和/或由ATP，荧光素和荧光素酶引起的生化发光反应的反应，并制备表示测量值与待测组分量之间的关系的校准曲线; 样品测量步骤，用与校准曲线制备步骤中使用的方法相同的方法测量待测样品; 以及量化步骤，通过使用校准曲线，从样品测量步骤中的测量值来求出待测样品中待测量的成分的量。