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公开(公告)号：US20150225768A1
公开(公告)日：2015-08-13
申请号：US14429784
申请日：2013-09-19
Applicant: DKK-TOA CORPORATION , HIROSHIMA UNIVERSITY
Inventor： Satoshi Yawata , Hiromitsu Hachiya , Akio Kuroda , Kenichi Noda
CPC classification number: C12Q1/66 , C12Q1/008 , G01N21/76 , G01N33/579 , G01N2400/24 , G01N2400/50
Abstract: A quantification method includes a calibration curve preparing step to measure a standard solution, which has been prepared by adding sodium ions so that a sodium ion content of the standard solution is equaled to a sodium ion content of a sample to be measured with a method employing a reaction that activates a limulus reagent and/or a biochemical luminescent reaction caused by ATP, luciferin, and luciferase, and to prepare a calibration curve that represents a relation between a measurement value and an amount of a component to be measured; a sample measuring step to measure the sample to be measured with a method being the same as that used in the calibration curve preparing step; and a quantifying step to find, by using the calibration curve, an amount of the component to be measured in the sample to be measured from a measurement value in the sample measuring step.
Abstract translation: 定量方法包括：通过添加钠离子制备标准溶液的标准溶液的校准曲线制备步骤，使标准溶液的钠离子含量与待测样品的钠离子含量相等， 激活鲎试剂和/或由ATP，荧光素和荧光素酶引起的生化发光反应的反应，并制备表示测量值与待测组分量之间的关系的校准曲线; 样品测量步骤，用与校准曲线制备步骤中使用的方法相同的方法测量待测样品; 以及量化步骤，通过使用校准曲线，从样品测量步骤中的测量值来求出待测样品中待测量的成分的量。
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公开(公告)号：US12180533B2
公开(公告)日：2024-12-31
申请号：US16976267
申请日：2019-03-01
Applicant: HIROSHIMA UNIVERSITY
Inventor： Ryuichi Hirota , Akio Kuroda , Kei Motomura
IPC: C12Q1/06 , C07K14/195 , C12N9/02 , C12N15/74
Abstract: It is an object of an aspect of the present invention to provide (i) transformants, whose proliferation depends on phosphite, of various species of organism and (ii) a method for detecting the presence of a reduced phosphorous compound with use of such a transformant. Use is made of a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced, a signal peptide of a hypophosphite binding protein being substituted with a signal peptide derived from a host or a species of organism closely related to the host.
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3.发明申请METHOD FOR SELECTIVELY CULTURING MICROORGANISM USING PHOSPHITE DEHYDROGENASE GENE AS MARKER 有权使用磷酸脱氢酶基因作为标记选择性培养微生物的方法公开(公告)号：US20150125934A1
公开(公告)日：2015-05-07
申请号：US14413035
申请日：2013-08-08
Applicant: Hiroshima University
Inventor： Akio Kuroda , Ryuichi Hirota , Takencri Ishida
IPC: C12N15/52 , C07K14/195 , C12N9/02
CPC classification number: C12N15/52 , C07K14/195 , C12N1/14 , C12N1/16 , C12N1/20 , C12N9/0004 , C12Y120/01001
Abstract: A simple and inexpensive method for selectively culturing a microorganism which method makes it possible to selectively culture a microorganism of interest even without using a sterilization operation or an antibiotic substance is provided. The method according to the present invention selectively culturing a microorganism includes the step of culturing, in a culture medium containing phosphorous acid as a sole phosphorous source, a recombinant microorganism into which a phosphite dehydrogenase gene has been introduced.
Abstract translation: 提供了一种用于选择性培养微生物的简单和便宜的方法，该方法使得即使不使用灭菌操作或抗生素也可选择性培养感兴趣的微生物。 根据本发明的选择性培养微生物的方法包括在含有磷酸作为唯一磷源的培养基中培养引入了亚磷酸脱氢酶基因的重组微生物的步骤。
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Patent Agency Ranking
公开(公告)号：US10253064B2
公开(公告)日：2019-04-09
申请号：US15521256
申请日：2015-10-21
Applicant: Hiroshima University
Inventor： Akio Kuroda , Takeshi Ikeda , Hisakage Funabashi
Abstract: The present invention provides a method for purifying an intended substance, a kit for purifying the intended substance, and a silicon oxide-binding tag for use in the method and the kit. In the present invention, a complex of a silicon oxide-binding tag and a substance is caused to specifically bind to silicon oxide in a solution for binding, to which solution no salt is added, and binding between the silicon oxide-binding tag and the silicon oxide is broken with use of arginine.
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公开(公告)号：US12117440B2
公开(公告)日：2024-10-15
申请号：US16640753
申请日：2018-07-25
Applicant: Hiroshima University
Inventor： Akio Kuroda , Takenori Ishida
IPC: G01N33/543 , C07K7/06
CPC classification number: G01N33/54326 , C07K7/06
Abstract: The present invention provides a method for simply isolating exosomes from a sample containing exosomes. The present invention includes a complex forming step of forming a complex by binding an exosome in a sample to a particular peptide that contains lysines and is supported on a carrier and a dissociating step of dissociating the exosome from the complex by bringing the complex into contact with a dissociation buffer containing metal cations.
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公开(公告)号：US08507216B2
公开(公告)日：2013-08-13
申请号：US13760524
申请日：2013-02-06
Applicant: Hiroshima University
Inventor： Akio Kuroda , Kenichi Noda
IPC: C12Q1/56
CPC classification number: G01N21/763 , C12Q1/66 , G01N33/56911 , G01N33/579
Abstract: The present invention provides a method comprising allowing a reaction of a sample, a reagent containing Factor C, which can be activated by binding with endotoxin, and a synthetic luminescent substrate comprising a luminescent substrate bound to a peptide, for release of the luminescent substrate from the synthetic luminescent substrate, allowing a luminescent enzyme to act on the luminescent substrate released in the luminescent substrate release step, for measurement of the luminescence intensity, and quantifying the level of endotoxin in the sample based on a measured value obtained in the luminescence measuring step, the method enabling endotoxin to be simply and quickly measured at a level that cannot be detected in conventional methods for endotoxin measurement, without use of any dedicated measuring device.
Abstract translation: 本发明提供了一种方法，其包括使样品，可通过与内毒素结合而活化的因子C的试剂与包含与肽结合的发光底物的合成发光底物反应，从而将发光底物从 所述合成发光基板允许发光酶作用于在所述发光底物释放步骤中释放的所述发光基板，用于测量所述发光强度，并且基于在所述发光测量步骤中获得的测量值来定量所述样品中的内毒素水平 ，在不使用任何专用测量装置的情况下，能够以常规的内毒素测量方法无法检测的水平简单且快速地测量内毒素的方法。
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公开(公告)号：US11130981B2
公开(公告)日：2021-09-28
申请号：US16328560
申请日：2017-07-31
Applicant: HIROSHIMA UNIVERSITY
Inventor： Ryuichi Hirota , Akio Kuroda
Abstract: In order to provide a transformant by which a high containment effect is obtained and which can be prepared by a simple procedure, a method for producing the transformant, and a method for detecting the presence of a reduced phosphorous compound with use of the transformant, a transformant in accordance with an embodiment of the present invention is a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced.
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8.发明授权Method for selectively culturing microorganism using phosphite dehydrogenase gene as marker 有权使用亚磷酸脱氢酶基因作为标记选择性培养微生物的方法公开(公告)号：US09499822B2
公开(公告)日：2016-11-22
申请号：US14413035
申请日：2013-08-08
Applicant: Hiroshima University
Inventor： Akio Kuroda , Ryuichi Hirota , Takenori Ishida
CPC classification number: C12N15/52 , C07K14/195 , C12N1/14 , C12N1/16 , C12N1/20 , C12N9/0004 , C12Y120/01001
Abstract: A simple and inexpensive method for selectively culturing a microorganism which method makes it possible to selectively culture a microorganism of interest even without using a sterilization operation or an antibiotic substance is provided. The method according to the present invention selectively culturing a microorganism includes the step of culturing, in a culture medium containing phosphorous acid as a sole phosphorous source, a recombinant microorganism into which a phosphite dehydrogenase gene has been introduced.
Abstract translation: 提供了一种用于选择性培养微生物的简单和便宜的方法，该方法使得即使不使用灭菌操作或抗生素也可选择性培养感兴趣的微生物。 根据本发明的选择性培养微生物的方法包括在含有磷酸作为唯一磷源的培养基中培养引入了亚磷酸脱氢酶基因的重组微生物的步骤。
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