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    METHOD OF PRODUCING A POLYPEPTIDE OR VIRUS OF INTEREST IN A CONTINUOUS CELL CULTURE
    1.
    发明申请
    METHOD OF PRODUCING A POLYPEPTIDE OR VIRUS OF INTEREST IN A CONTINUOUS CELL CULTURE 有权
    在连续细胞培养中生产多肽或感兴趣的病毒的方法

    公开(公告)号：US20140038264A1

    公开(公告)日：2014-02-06

    申请号：US14050710

    申请日：2013-10-10

    申请人： Baxter International Inc. ,  Baxter Healthcare S.A.

    发明人： Leopold Grillberger ,  Manfred Reiter ,  Daniel Fleischanderl

    IPC分类号： C12N7/00

    CPC分类号： C12N7/00 ,  C12N9/6489 ,  C12N2710/00052 ,  C12P21/02 ,  Y02P20/52

    摘要： Described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d−1, and a cell density of less than about 2×107 cell/mL. Also described herein is a method for producing a polypeptide and/or virus of interest in a continuous cell culture, the method comprising culturing mammalian cells expressing the polypeptide and/or virus of interest in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d−1, and a cell density of less than about 2×107 cell/mL; and recovering the polypeptide and/or virus of interest from medium of the cell culture system.

    摘要翻译： 本文描述的是一种恒化器样连续细胞培养系统，其结合了灌注开放系统和恒化器开放系统的某些优点，以改善哺乳动物细胞，例如遗传修饰的细胞，特别是在无血清或化学定义的培养基中的培养。 本文所述的连续培养系统包括在包含细胞保留装置的连续细胞培养系统中培养哺乳动物细胞，其中细胞培养系统的稀释速率（D）小于约2d-1，细胞密度 小于约2×10 7个细胞/ mL。 本文还描述了在连续细胞培养物中产生感兴趣的多肽和/或病毒的方法，所述方法包括在连续细胞培养系统中培养表达所述多肽和/或感兴趣的病毒的哺乳动物细胞，其包含细胞保留装置 其中细胞培养体系具有小于约2d-1的稀释倍率（D），小于约2×10 7个细胞/ mL的细胞密度; 以及从细胞培养系统的培养基中回收目标多肽和/或病毒。

    Animal protein-free media for cultivation of cells
    2.
    发明授权
    Animal protein-free media for cultivation of cells 有权
    用于培养细胞的动物无蛋白质培养基

    公开(公告)号：US08748156B2

    公开(公告)日：2014-06-10

    申请号：US13864118

    申请日：2013-04-16

    申请人： Baxter International Inc. ,  Baxter Healthcare S.A.

    发明人： Leopold Grillberger ,  Manfred Reiter ,  Wolfgang Mundt ,  Friedrich Dorner

    IPC分类号： C12N1/20 ,  C07H21/04

    CPC分类号： C12N5/0043 ,  C12N5/0682 ,  C12N2500/46 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/92

    摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

    摘要翻译： 本发明涉及含有多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。 本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。 这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。

    Animal protein-free media for cultivation of cells
    9.
    发明授权
    Animal protein-free media for cultivation of cells 有权
    用于培养细胞的动物无蛋白质培养基

    公开(公告)号：US09222075B2

    公开(公告)日：2015-12-29

    申请号：US14268859

    申请日：2014-05-02

    申请人： Baxter International Inc. ,  Baxter Healthcare SA

    发明人： Leopold Grillberger ,  Manfred Reiter ,  Wolfgang Mundt ,  Friedrich Dorner

    IPC分类号： C12N1/20 ,  C12N5/071 ,  C12N5/00

    CPC分类号： C12N5/0043 ,  C12N5/0682 ,  C12N2500/46 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/92

    摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

    摘要翻译： 本发明涉及含有多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。 本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。 这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。

    ANIMAL PROTEIN-FREE MEDIA FOR CULTIVATION OF CELLS
    10.
    发明申请
    ANIMAL PROTEIN-FREE MEDIA FOR CULTIVATION OF CELLS 有权
    用于培养细胞的动物蛋白质免费媒体

    公开(公告)号：US20150104867A1

    公开(公告)日：2015-04-16

    申请号：US14268859

    申请日：2014-05-02

    申请人： Baxter International Inc. ,  Baxter Healthcare SA

    发明人： Leopold Grillberger ,  Manfred Reiter ,  Wolfgang Mundt ,  Friedrich Dorner

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0043 ,  C12N5/0682 ,  C12N2500/46 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/92

    摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

    摘要翻译： 本发明涉及含有多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。 本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。 这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。