公开(公告)号：US11340230B2

公开(公告)日：2022-05-24

申请号：US17071976

申请日：2020-10-15

申请人： Abbott Laboratories

发明人： Anthony S. Muerhoff ,  John C. Prostko ,  Christopher C. Marohnic

IPC分类号： G01N33/576

摘要： The present disclosure provides methods, kits, and compositions for detecting subject anti-HCV antibodies in a sample using NS3 capture peptides. In certain embodiments, at least two NS3 helicase (NS3h) capture peptides and at least two conjugate peptides (e.g., NS3h conjugate peptides) are employed together, which allows for a broad dynamic range of subject antibody detection in a one-step type assay. In other embodiments, methods are provided of detecting NS3-specific subject antibodies without the use of a reducing agent. In some embodiments, NS3-specific subject antibodies are detected with a ‘double shot’ of NS3 conjugate peptide (e.g., conjugate peptide added to a sample both before and after washing).

公开(公告)号：US10308709B2

公开(公告)日：2019-06-04

申请号：US15290762

申请日：2016-10-11

申请人： ABBOTT LABORATORIES

发明人： Bailin Tu ,  Robert N. Ziemann ,  Bryan C. Tieman ,  Philip M. Hemken ,  Carol S. Ramsay ,  Carolyn J. Strobel ,  David J. Hawksworth ,  Larry G. Birkenmeyer ,  Cheng Zhao ,  Susan E. Brophy ,  Barry L. Dowell ,  Anthony S. Muerhoff

IPC分类号： G01N33/53 ,  C07K16/18 ,  G01N33/574 ,  G01N33/68

摘要： Disclosed herein are antibodies and methods of using said antibodies to detect Golgi protein 73 (GP73) and fucosylated GP73 in a sample.

公开(公告)号：US09469686B2

公开(公告)日：2016-10-18

申请号：US13836229

申请日：2013-03-15

申请人： Abbott Laboratories

发明人： Bailin Tu ,  Robert N. Ziemann ,  Bryan C. Tieman ,  Philip M. Hemken ,  Carol S. Ramsay ,  Carolyn J. Strobel ,  David J. Hawksworth ,  Larry G. Birkenmeyer ,  Cheng Zhao ,  Susan E. Brophy ,  Barry L. Dowell ,  Anthony S. Muerhoff

IPC分类号： C07K16/00 ,  A61K39/395 ,  C07K16/18 ,  G01N33/574 ,  G01N33/68

CPC分类号： C07K16/18 ,  C07K2317/21 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  G01N33/57438 ,  G01N33/6893 ,  G01N2333/47 ,  G01N2800/085 ,  G01N2800/50

摘要： Disclosed herein are antibodies and methods of using said antibodies to detect Golgi protein 73 (GP73) and fucosylated GP73 in a sample.

摘要翻译： 本文公开了使用所述抗体检测样品中的高尔基体蛋白73（GP73）和岩藻糖基化GP73的抗体和方法。

公开(公告)号：US20190315844A1

公开(公告)日：2019-10-17

申请号：US16387282

申请日：2019-04-17

申请人： ABBOTT LABORATORIES

发明人： Bailin Tu ,  Robert N. Ziemann ,  Bryan C. Tieman ,  Philip M. Hemken ,  Carol S. Ramsay ,  Carolyn J. Strobel ,  David J. Hawksworth ,  Larry G. Birkenmeyer ,  Cheng Zhao ,  Susan E. Brophy ,  Barry L. Dowell ,  Anthony S. Muerhoff

IPC分类号： C07K16/18 ,  G01N33/574 ,  G01N33/68

摘要： Disclosed herein are antibodies and methods of using said antibodies to detect Golgi protein 73 (GP73) and fucosylated GP73 in a sample.

公开(公告)号：US10816551B2

公开(公告)日：2020-10-27

申请号：US16122261

申请日：2018-09-05

申请人： Abbott Laboratories

发明人： Anthony S. Muerhoff ,  John C. Prostko ,  Christopher C. Marohnic

IPC分类号： G01N33/576

摘要： The present disclosure provides methods, kits, and compositions for detecting subject anti-HCV antibodies in a sample using NS3 capture peptides. In certain embodiments, at least two NS3 helicase (NS3h) capture peptides and at least two conjugate peptides (e.g., NS3h conjugate peptides) are employed together, which allows for a broad dynamic range of subject antibody detection in a one-step type assay. In other embodiments, methods are provided of detecting NS3-specific subject antibodies without the use of a reducing agent. In some embodiments, NS3-specific subject antibodies are detected with a ‘double shot’ of NS3 conjugate peptide (e.g., conjugate peptide added to a sample both before and after washing).

公开(公告)号：US20200256843A1

公开(公告)日：2020-08-13

申请号：US16864672

申请日：2020-05-01

申请人： ABBOTT LABORATORIES

发明人： Jeffrey B. Huff ,  Mark A. Hayden ,  Peter J. Karabatsos ,  Andrew S. Schapals ,  Anthony S. Muerhoff ,  M. Felicia Bogdan ,  Thomas Leary ,  Shelley R. Holets-McCormack ,  Sophie Laurenson ,  Andrew T. Fischer ,  Richard Haack ,  Stefan Hershberger ,  Dustin House ,  Lei Qiao ,  M. Shawn Murphy ,  Mark R. Pope ,  Edna M. Prieto-Ballengee ,  QiaoQiao Ruan ,  Pathik Soni ,  Sergey Tetin ,  Lyle Yarnell

IPC分类号： G01N33/487 ,  G01N33/53 ,  B01L3/00 ,  G01N33/543

摘要： Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

公开(公告)号：US20160131653A1

公开(公告)日：2016-05-12

申请号：US14925778

申请日：2015-10-28

申请人： Abbott Laboratories

发明人： Anthony S. Muerhoff ,  John Prostko ,  Chris Marohnic

IPC分类号： G01N33/576

CPC分类号： G01N33/5767 ,  G01N2333/186 ,  G01N2333/914 ,  G01N2469/20

摘要： The present disclosure provides methods, kits, and compositions for detecting subject anti-HCV antibodies in a sample using NS3 capture peptides. In certain embodiments, at least two NS3 helicase (NS3h) capture peptides and at least two conjugate peptides (e.g., NS3h conjugate peptides) are employed together, which allows for a broad dynamic range of subject antibody detection in a one-step type assay. In other embodiments, methods are provided of detecting NS3-specific subject antibodies without the use of a reducing agent. In some embodiments, NS3-specific subject antibodies are detected with a ‘double shot’ of NS3 conjugate peptide (e.g., conjugate peptide added to a sample both before and after washing).

摘要翻译： 本公开提供了使用NS3捕获肽检测样品中的受试者抗HCV抗体的方法，试剂盒和组合物。 在某些实施方案中，一起使用至少两种NS3解旋酶（NS3h）捕获肽和至少两种缀合肽（例如NS3h缀合肽），其允许在一步式测定中的主题抗体检测的宽动态范围。 在其它实施方案中，提供了在不使用还原剂的情况下检测NS3特异性目标抗体的方法。 在一些实施方案中，NS3特异性目标抗体用NS3缀合肽“双重”检测（例如，在洗涤前后添加到样品中的结合肽）。

公开(公告)号：US11022598B2

公开(公告)日：2021-06-01

申请号：US15724213

申请日：2017-10-03

申请人： ABBOTT LABORATORIES

发明人： Jeffrey B. Huff ,  Mark A. Hayden ,  Peter J. Karabatsos ,  Andrew S. Schapals ,  Anthony S. Muerhoff ,  M. Felicia Bogdan ,  Thomas Leary ,  Shelley R. Holets-McCormack ,  Sophie Laurenson ,  Andrew T. Fischer ,  Richard Haack ,  Stefan Hershberger ,  Dustin House ,  Lei Qiao ,  M. Shawn Murphy ,  Mark R. Pope ,  Edna M. Prieto-Ballengee ,  QiaoQiao Ruan ,  Pathik Soni ,  Sergey Tetin ,  Lyle Yarnell

IPC分类号： G01N33/487 ,  G01N33/543 ,  G01N33/53 ,  B01L3/00

摘要： Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

公开(公告)号：US20190064169A1

公开(公告)日：2019-02-28

申请号：US16122261

申请日：2018-09-05

申请人： Abbott Laboratories

发明人： Anthony S. Muerhoff ,  John C. Prostko ,  Christopher C. Marohnic

IPC分类号： G01N33/576

CPC分类号： G01N33/5767 ,  G01N2333/186 ,  G01N2333/914 ,  G01N2469/20

摘要： The present disclosure provides methods, kits, and compositions for detecting subject anti-HCV antibodies in a sample using NS3 capture peptides. In certain embodiments, at least two NS3 helicase (NS3h) capture peptides and at least two conjugate peptides (e.g., NS3h conjugate peptides) are employed together, which allows for a broad dynamic range of subject antibody detection in a one-step type assay. In other embodiments, methods are provided of detecting NS3-specific subject antibodies without the use of a reducing agent. In some embodiments, NS3-specific subject antibodies are detected with a ‘double shot’ of NS3 conjugate peptide (e.g., conjugate peptide added to a sample both before and after washing).

公开(公告)号：US10088483B2

公开(公告)日：2018-10-02

申请号：US14925778

申请日：2015-10-28

申请人： Abbott Laboratories

发明人： Anthony S. Muerhoff ,  John Prostko ,  Chris Marohnic

IPC分类号： G01N33/576

摘要： The present disclosure provides methods, kits, and compositions for detecting subject anti-HCV antibodies in a sample using NS3 capture peptides. In certain embodiments, at least two NS3 helicase (NS3h) capture peptides and at least two conjugate peptides (e.g., NS3h conjugate peptides) are employed together, which allows for a broad dynamic range of subject antibody detection in a one-step type assay. In other embodiments, methods are provided of detecting NS3-specific subject antibodies without the use of a reducing agent. In some embodiments, NS3-specific subject antibodies are detected with a ‘double shot’ of NS3 conjugate peptide (e.g., conjugate peptide added to a sample both before and after washing).