公开(公告)号：US08243266B2

公开(公告)日：2012-08-14

申请号：US12788136

申请日：2010-05-26

申请人： Kohei Watanabe ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yasuhito Shimada

发明人： Kohei Watanabe ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yasuhito Shimada

IPC分类号： G01N1/10

CPC分类号： G01N35/00

摘要： A sample observation system and a sample observation process are provided which do not need any special manipulation or treatment to limit the motion of the sample or adjust the orientation of the sample when observing the sample, and enable observation of samples simply from wider direction than ever. A sample observation system of a sample whose vertical direction is defined by gravity, wherein the system at least includes a sample holder having at least one penetrating hole for retaining a liquid containing the sample; an observation unit for observing the sample; and an angle adjusting unit for varying the angle of the observation unit to the direction of the gravity which acts on the sample in a condition in which the sample holder and the observation unit have a fixed relative angle therebetween.

摘要翻译： 提供样品观察系统和样品观察过程，其不需要任何特殊的操作或处理来限制样品的运动或在观察样品时调整样品的取向，并且能够简单地从宽的方向观察样品 。 样品的样品观察系统，其垂直方向由重力限定，其中所述系统至少包括具有至少一个用于保持含有样品的液体的穿透孔的样品保持器; 用于观察样品的观察单元; 以及角度调整单元，用于在样本保持器和观察单元之间具有固定的相对角度的状态下，改变观察单元与作用在样本上的重力方向的角度。

公开(公告)号：US20110243850A1

公开(公告)日：2011-10-06

申请号：US13133381

申请日：2009-12-24

申请人： Taichi Shintou ,  Takeshi Miyazaki ,  Masashi Hirose ,  Taketoshi Okubo ,  Kohei Watanabe ,  Tsuyoshi Nomoto ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

发明人： Taichi Shintou ,  Takeshi Miyazaki ,  Masashi Hirose ,  Taketoshi Okubo ,  Kohei Watanabe ,  Tsuyoshi Nomoto ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

IPC分类号： A61K49/00 ,  C07D417/10 ,  C07D417/14 ,  C07D209/94 ,  C07D403/10 ,  C07D413/10 ,  C07D409/10 ,  C07D401/10 ,  C07D405/10 ,  G01N1/30

CPC分类号： A61K51/0453 ,  A61K49/0002 ,  A61K49/0008 ,  A61K49/0021 ,  A61K49/006 ,  A61K49/10 ,  C07D209/08 ,  C07D209/86 ,  C07D209/94 ,  C07D401/08 ,  C07D403/06 ,  C07D403/08 ,  C07D413/08 ,  C07D417/06 ,  C07D417/14 ,  C09B23/0091 ,  C09B23/04 ,  C09B23/105 ,  G01N33/582 ,  G01N2333/4603 ,  G01N2500/10

摘要： Provided is a novel probe for a biological specimen for labelling by itself and clearly visualizing one of a specific cell and a specific cell organ in a living body, the probe having excellent spectral characteristics and exhibiting excellent storage stability. The probe for a biological specimen contains, as an active agent, at least one kind of compound represented by a general formula (I).

摘要翻译： 本发明提供了一种用于标记本身并且清楚地观察生物体中的特定细胞和特定细胞器官之一的生物样本的新型探针，所述探针具有优异的光谱特性并且显示出优异的储存稳定性。 用于生物样品的探针作为活性剂含有至少一种由通式（I）表示的化合物。

公开(公告)号：US20110236310A1

公开(公告)日：2011-09-29

申请号：US13133357

申请日：2009-12-24

申请人： Kohei Watanabe ,  Taichi Shintou ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

发明人： Kohei Watanabe ,  Taichi Shintou ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

IPC分类号： A61K49/00 ,  C07D221/18 ,  A61K51/04 ,  C07D491/14 ,  C09B1/54 ,  C07D311/82 ,  C07D417/06 ,  A61K51/08

CPC分类号： A61K49/0021 ,  A61K49/0002 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/006 ,  C07D209/70 ,  C07D209/96 ,  C07D215/00 ,  C07D221/14 ,  C07D221/18 ,  C07D263/54 ,  C07D277/34 ,  C07D277/36 ,  C07D277/64 ,  C07D311/82 ,  C07D311/86 ,  C07D405/04 ,  C07D405/06 ,  C07D413/04 ,  C07D413/06 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C07D491/052 ,  C07D491/12 ,  C07D497/10 ,  G01N33/5088 ,  G01N33/582 ,  G01N2333/4603

摘要： The invention provides a labeling composition for an intraocular tissue of a living individual, which specifically labels the intraocular tissue without need of an invasive operation such as exposure of an ocular tissue or injection of a staining agent into the ocular tissue or a nerve tissue linking to the ocular tissue, a method of noninvasively labeling an intraocular tissue of a living individual, and a screening method using the labeling composition for the intraocular tissues. The composition contains a compound capable of labeling at least a photoreceptor cell layer of a retina, wherein the compound is a staining compound having a particular structure as a partial structure thereof.

摘要翻译： 本发明提供了一种用于生物个体的眼内组织的标记组合物，其特异性地标记眼内组织，而不需要诸如眼组织的暴露或注射染色剂的侵入性手术或连接到眼组织的神经组织 眼组织，活体标记眼内组织的方法，以及使用眼内组织标记组合物的筛选方法。 该组合物含有能够至少标记视网膜感光细胞层的化合物，其中该化合物是具有特定结构的染色化合物作为其部分结构。

公开(公告)号：US07989219B2

公开(公告)日：2011-08-02

申请号：US11913045

申请日：2006-05-30

申请人： Hidenori Shiotsuka ,  Satoru Hatakeyama ,  Tsuyoshi Nomoto ,  Masaru Kaieda ,  Junta Yamamichi

发明人： Hidenori Shiotsuka ,  Satoru Hatakeyama ,  Tsuyoshi Nomoto ,  Masaru Kaieda ,  Junta Yamamichi

IPC分类号： G01N33/53

CPC分类号： C07K16/40 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2319/73 ,  G01N33/531 ,  G01N33/566 ,  G01N33/6854 ,  Y10S435/97 ,  Y10S436/81

摘要： A capturing molecule having an association containing a plurality of polypeptide chains that specifically bind to different sites of a target substance, characterized in that each of the polypeptide chains has a domain having a hypervariable loop structure at a binding site binding to the target substance and an association portion for forming the association, and the polypeptide chains are associated via the association portions present in the polypeptide chains.

摘要翻译： 具有包含特异性结合靶物质的不同位点的多个多肽链的缔合的捕获分子，其特征在于，每个多肽链具有在结合位点与目标物质具有高变环结构的结构域，以及 用于形成缔合的缔合部分和多肽链通过存在于多肽链中的缔合部分相关联。

公开(公告)号：US20110182810A1

公开(公告)日：2011-07-28

申请号：US13084971

申请日：2011-04-12

申请人： Tsuyoshi Nomoto ,  Kohei Watanabe ,  Taichi Shintou ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada

发明人： Tsuyoshi Nomoto ,  Kohei Watanabe ,  Taichi Shintou ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada

IPC分类号： A61K51/04 ,  A61K49/10

CPC分类号： A61K49/0004 ,  A61K49/0032 ,  A61K49/0041 ,  A61K51/0446

摘要： There is provided a central nervous system labelling composition for intranasal administration for the purpose of labelling the central nervous system from the olfactory epithelium by way of the olfactory bulb and by means of intranasal administration. Additionally, there is provided a method of non-invasively labelling the central nervous system by way of an administration route that entails little transferability to the entire body. Furthermore, there is provided a screening method using a central nervous system labelling composition for intranasal administration. A central nervous system labelling composition for intranasal administration is characterized by labelling the central nervous system from the olfactory epithelium by way of the olfactory bulb and by means of intranasal administration and by containing at least one compound expressed either by the general formula (1) or the general formula (2) shown below as effective component:

摘要翻译： 提供了用于鼻内给药的中枢神经系统标记组合物，用于通过嗅球通过鼻涕管理从嗅觉上皮标记中枢神经系统的目的。 此外，提供了一种通过对整个身体几乎不具有转移性的给药途径非侵入性地标记中枢神经系统的方法。 此外，提供了一种使用中枢神经系统标记组合物进行鼻内给药的筛选方法。 用于鼻内给药的中枢神经系统标记组合物的特征在于通过嗅球标记嗅觉上皮的中枢神经系统，并通过鼻内给药并含有至少一种由通式（1）或 以下所示的通式（2）为有效成分：

公开(公告)号：US07919263B2

公开(公告)日：2011-04-05

申请号：US10548442

申请日：2004-08-18

申请人： Hidenori Shiotsuka ,  Takeshi Imamura ,  Tsuyoshi Nomoto ,  Miki Ogawa

发明人： Hidenori Shiotsuka ,  Takeshi Imamura ,  Tsuyoshi Nomoto ,  Miki Ogawa

IPC分类号： G01N31/00 ,  G01N33/53

CPC分类号： C07K17/14 ,  C07K7/08 ,  C07K17/08 ,  C12N11/14 ,  G01N33/54353 ,  G01N33/552

摘要： The invention provides an organic material-immobilizing structure employing new immobilization means, characterized in that at least a part of the surface of the substrate is comprised of one or more members containing silicon oxide, the organic material is bound to the surface of the substrate through a binding domain bound to the organic material and containing an amino acid sequence capable of binding to silicon oxide, selected from the group consisting of amino acid sequences of SEQ ID NOS: 1 and 2: Val-Ser-Pro-Met-Arg-Ser-Ala-Thr-Thr-His-Thr-Val; and Ile-Pro-Met-His-Val-His-His-Lys-His-Pro-His-Val, and derivatives thereof.

摘要翻译： 本发明提供一种使用新的固定装置的有机材料固定结构，其特征在于，基底表面的至少一部分由含有氧化硅的一种或多种构件组成，有机材料通过 与有机材料结合并含有能够结合氧化硅的氨基酸序列的结合结构域，其选自SEQ ID NO：1和2的氨基酸序列：Val-Ser-Pro-Met-Arg-Ser -Ala-Thr-Thr-His-Thr-Val; 和Ile-Pro-Met-His-Val-His-His-Lys-His-Pro-His-Val及其衍生物。

公开(公告)号：US20100302534A1

公开(公告)日：2010-12-02

申请号：US12788136

申请日：2010-05-26

申请人： Kohei Watanabe ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yasuhito Shimada

发明人： Kohei Watanabe ,  Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Toshio Tanaka ,  Yasuhito Shimada

IPC分类号： G01N1/10

CPC分类号： G01N35/00

摘要： A sample observation system and a sample observation process are provided which do not need any special manipulation or treatment to limit the motion of the sample or adjust the orientation of the sample when observing the sample, and enable observation of samples simply from wider direction than ever. A sample observation system of a sample whose vertical direction is defined by gravity, wherein the system at least includes a sample holder having at least one penetrating hole for retaining a liquid containing the sample; an observation unit for observing the sample; and an angle adjusting unit for varying the angle of the observation unit to the direction of the gravity which acts on the sample in a condition in which the sample holder and the observation unit have a fixed relative angle therebetween.

摘要翻译： 提供样品观察系统和样品观察过程，其不需要任何特殊的操作或处理来限制样品的运动或在观察样品时调整样品的取向，并且能够简单地从宽的方向观察样品 。 样品的样品观察系统，其垂直方向由重力限定，其中所述系统至少包括具有至少一个用于保持含有样品的液体的穿透孔的样品保持器; 用于观察样品的观察单元; 以及角度调整单元，用于在样本保持器和观察单元之间具有固定的相对角度的状态下，改变观察单元与作用在样本上的重力方向的角度。

公开(公告)号：US20100166663A1

公开(公告)日：2010-07-01

申请号：US12644472

申请日：2009-12-22

申请人： Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Kohei Watanabe ,  Taichi Shintou ,  Kaoru Takahashi ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

发明人： Tsuyoshi Nomoto ,  Takeshi Miyazaki ,  Kohei Watanabe ,  Taichi Shintou ,  Kaoru Takahashi ,  Toshio Tanaka ,  Yuhei Nishimura ,  Yasuhito Shimada ,  Norihiro Nishimura

IPC分类号： A61K49/00 ,  A61P27/16

CPC分类号： G01N33/5005 ,  A61K49/0032 ,  A61K49/0041 ,  A61K49/006 ,  C09B11/24 ,  C09B23/0016 ,  C09B23/0058 ,  C09B23/0066 ,  C09B23/0075 ,  C09B23/06 ,  C09B23/105 ,  C09B23/107 ,  G01N33/5044 ,  G01N33/6893 ,  G01N2800/14 ,  G01N2800/20

摘要： Provided are a novel probe for a hair cell for clearly identifying various conditions of a hair cell, and a labelling method for a hair cell using the probe for a hair cell, more particularly, a probe for a hair cell containing, as an active agent, at least one kind selected from staining compounds represented by one of the general formulae (I) and (II), and a labelling method for a hair cell using the probe for a hair cell.

摘要翻译： 本发明提供一种用于毛细胞的新型探针，用于清楚地识别毛细胞的各种条件，以及使用毛细胞探针的毛细胞的标记方法，更具体地，涉及含有作为活性成分的毛细胞的探针 ，选自由通式（I）和（II）之一表示的染色化合物中的至少一种，以及使用毛细胞探针的毛细胞的标记方法。

公开(公告)号：US07632618B2

公开(公告)日：2009-12-15

申请号：US12177094

申请日：2008-07-21

申请人： Tsuyoshi Nomoto ,  Tetsuya Yano ,  Shinya Kozaki ,  Tsutomu Honma

发明人： Tsuyoshi Nomoto ,  Tetsuya Yano ,  Shinya Kozaki ,  Tsutomu Honma

IPC分类号： C03G9/00 ,  C08G63/06 ,  C12N11/14 ,  C12P7/40

CPC分类号： C12N9/1025 ,  C12N11/00 ,  C12P7/625 ,  Y10T428/2989

摘要： A method for manufacturing polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with polyhydroxyalkanoate, the method comprises the steps of immobilizing a polyhydroxyalkanoate synthase on the base material surface, synthesizing, on the base material surface, polyhydroxyalkanoate using a 3-hydroxyacyl coenzyme A to become the substrate of the synthase and the synthase and coating at least a part of the base material surface with the synthesized polyhydroxyalkanoate, wherein the synthase contains an amino acid sequence capable of binding to the base material. A polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with a polyhydroxyalkanoate, comprises the base material, a polyhydroxyalkanoate synthase immobilized on the base material surface, and the polyhydroxyalkanoate with which at least a part of the base material surface is coated, wherein the synthase contains an amino acid sequence capable of binding to the base material.

摘要翻译： 一种制备聚羟基链烷酸酯结构的方法，所述结构的基材表面的至少一部分涂覆有聚羟基链烷酸酯，所述方法包括以下步骤：将聚羟基链烷酸酯合酶固定在基材表面上，在基材表面上合成， 聚羟基链烷酸酯使用3-羟酰基辅酶A成为合成酶和合成酶的底物并用合成的聚羟基链烷酸酯涂覆至少一部分基材表面，其中合成酶含有能够与基材结合的氨基酸序列。 一种含聚羟基链烷酸酯的结构，该结构的基材表面的至少一部分涂覆有聚羟基链烷酸酯，其包含基材，固定在基材表面上的聚羟基链烷酸酯合酶和至少一部分的聚羟基链烷酸酯 基材表面被涂覆，其中合成酶含有能够结合基材的氨基酸序列。

公开(公告)号：US07632600B2

公开(公告)日：2009-12-15

申请号：US11525848

申请日：2006-09-25

申请人： Wataru Kubo ,  Tsuyoshi Nomoto ,  Tetsuya Yano ,  Rie Ueno

发明人： Wataru Kubo ,  Tsuyoshi Nomoto ,  Tetsuya Yano ,  Rie Ueno

IPC分类号： H01M4/00 ,  H01M4/88 ,  C12N11/00

CPC分类号： H01M8/16 ,  H01M4/8803 ,  H01M4/8817 ,  H01M4/8828 ,  H01M4/8882 ,  H01M4/9008 ,  Y02E60/527

摘要： An enzyme electrode that enables to enhance the rate of charge transfer from a redox center of an enzyme and catalytic current is provided. An enzyme electrode capable of increasing catalytic current by increasing the rate of charge transfer from an enzyme using an enzyme/metal fine particle complex in which part of a metal fine particle is incorporated into the enzyme can be provided.

摘要翻译： 提供能够提高从酶的氧化还原中心的电荷转移速率和催化电流的酶电极。 可以提供能够通过增加使用其中部分金属微粒掺入到酶中的酶/金属微粒络合物从酶的电荷转移速率来增加催化电流的酶电极。