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    Systems and methods for identifying bioactive agents utilizing unbiased machine learning
    3.
    发明授权
    Systems and methods for identifying bioactive agents utilizing unbiased machine learning 有权

    公开(公告)号：US12141963B2

    公开(公告)日：2024-11-12

    申请号：US17282039

    申请日：2019-10-04

    Applicant: THE ROCKEFELLER UNIVERSITY

    Inventor： Jakob Metzger ,  Fred Etoc ,  Ali Brivanlou ,  Eric Siggia

    IPC: G06T7/62 ,  C12N5/0735 ,  G01N1/30 ,  G01N33/50 ,  G06F18/21 ,  G06F18/214 ,  G06N3/084 ,  G06T7/00 ,  G06V10/764 ,  G06V10/82 ,  G06V20/69 ,  G16B40/00

    Abstract: Systems and methods for identifying molecules that are biologically active against a disease, where the method can comprise culturing a first mammalian cell population under organoid formation conditions in the presence of a test molecule to obtain a first organoid, wherein the first mammalian cell population, when cultured under the organoid formation conditions in the absence of the test molecule, results in an organoid with a disease phenotype; imaging the first organoid following exposure to the test molecule; analyzing one or more images of the first organoid using a neural network that has been trained to assign a probability score of disease or non-disease ranging between 0% and 100%; assigning the first organoid a probability score ranging between 0% and 100%; wherein the test molecule is biologically active against the disease if the probability score of the first organoid is greater than a cutoff probability score of non-disease or lower than a cutoff probability score of disease.

    Hybrid multi-photon microscopy
    7.
    发明授权
    Hybrid multi-photon microscopy 有权

    公开(公告)号：US11808702B2

    公开(公告)日：2023-11-07

    申请号：US17427586

    申请日：2020-01-30

    Applicant: The Rockefeller University

    Inventor： Alipasha Vaziri ,  Siegfried Weisenburger

    IPC: G01N21/64 ,  G02B21/16

    CPC classification number: G01N21/6428 ,  G01N21/6458 ,  G02B21/16 ,  G01N2201/06113

    Abstract: A multi-photon imaging system includes a laser module having a first channel for outputting a two-photon excitation laser pulse and a second channel for outputting a three-photon excitation laser pulse. The system further includes a first optical path for guiding the two-photon laser pulse from the first channel of the laser module and a second optical path for guiding the three-photon laser pulse from the second channel of the laser module. A microscope is also provided for simultaneously receiving the two-photon laser pulse from the first optical path and the three-photon laser pulse from the second optical path, and simultaneously, or with well controllable delays, delivering the two-photon laser pulse and the three-photon pulse to a target volume. The system further includes a photodetector configured to collect photons generated within the target volume in response to simultaneous excitation of the target volume by both the two-photon laser pulse and the three-photon laser pulse.

    SEQUENCE SPECIFIC DEGRADATION OF SINGLE-STRANDED POLYNUCLEOTIDES WITH CARD1 NUCLEASE
    10.
    发明公开
    SEQUENCE SPECIFIC DEGRADATION OF SINGLE-STRANDED POLYNUCLEOTIDES WITH CARD1 NUCLEASE 审中-公开

    公开(公告)号：US20230287371A1

    公开(公告)日：2023-09-14

    申请号：US18006251

    申请日：2021-07-19

    Applicant: The Rockefeller University

    Inventor： Luciano MARRAFFINI ,  Jakob ROSTOL

    IPC: C12N9/22 ,  C12N15/11

    CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N2310/20

    Abstract: Provided is an isolated or recombinantly expressed protein comprising the sequence of SEQ ID NO: 1 (CARD1), or an amino acid sequence that is at least 90% identical to the sequence of CARD1. Methods for producing and isolating the CARD1 protein are provided. Also provided are methods that include using CARD1, Cas1O and RNA obtained from a biological sample, and a guide RNA targeted to an RNA polynucleotide that may be in the biological sample, and determining whether or not the CARD1 cleaves a reporter ssDNA or reporter ssRNA that is added to the sample, to determine the presence or absence of the RNA polynucleotide. Kits for use in the assay are also provided.

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