公开(公告)号：US08795628B2

公开(公告)日：2014-08-05

申请号：US12598780

申请日：2008-05-05

Applicant: Sanjiv S. Gambhir ,  Shay Keren ,  Ian Walton ,  David Guagliardo

Inventor： Sanjiv S. Gambhir ,  Shay Keren ,  Ian Walton ,  David Guagliardo

IPC: A61K51/00 ,  A61K49/00

CPC classification number: G01N21/658 ,  B82Y30/00

Abstract: Methods of imaging a living host using Raman nanoparticles; methods of generating a true image of a living host having been administered Raman nanoparticles; methods of multiplex imaging of a living host using a plurality of Raman nanoparticles; methods of generating multimodality images by combining Raman images with other functional/anatomical images; labeled Raman nanoparticles; and the like, are provided.

Abstract translation: 使用拉曼纳米颗粒成像活体的方法; 产生已经施用拉曼纳米粒子的活体的真实图像的方法; 使用多个拉曼纳米粒子对活体进行多重成像的方法; 通过将拉曼图像与其他功能/解剖图像组合来产生多模态图像的方法; 标记的拉曼纳米颗粒; 等等。

公开(公告)号：US20120295326A1

公开(公告)日：2012-11-22

申请号：US13562881

申请日：2012-07-31

Applicant: Sanjiv S. Gambhir ,  Andreas M. Loening ,  Anna M. Wu

Inventor： Sanjiv S. Gambhir ,  Andreas M. Loening ,  Anna M. Wu

IPC: C12N9/02 ,  C12N1/21 ,  C12N1/19 ,  C12N15/63 ,  C12N5/10

CPC classification number: C12Q1/66

Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.

Abstract translation: 简要描述，本公开的实施方案包括编码与相应的野生型萤光素酶相比表现出调节特性的突变型雀巢萤光素酶的多核苷酸，并且调节的性质包括以下至少一种：调节的稳定性; 增强光输出; 并调制发射最大值。 本公开的实施方案还包括由多核苷酸编码的多肽或其片段，包括多核苷酸的构建体，表达盒，细胞，产生多核苷酸和多肽的方法，抗体，转基因细胞和/或动物，试剂盒等。

公开(公告)号：US08178654B2

公开(公告)日：2012-05-15

申请号：US11805460

申请日：2007-05-23

Applicant: Sanjiv S. Gambhir ,  Ramasamy Paulmurugan

Inventor： Sanjiv S. Gambhir ,  Ramasamy Paulmurugan

IPC: C07K14/705

CPC classification number: C07K14/721 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/0331 ,  G01N33/542 ,  G01N2333/723

Abstract: Briefly described, embodiments of this disclosure include estrogen receptor (ER) intramolecular folding systems, methods of detecting ER ligands and distinguishing between ER agonists and antagonists, cells including ER intramolecular folding systems, transgenic animals including ER intramolecular folding systems, fusion proteins, and the like.

Abstract translation: 简要描述，本公开的实施方案包括雌激素受体（ER）分子内折叠系统，检测ER配体和区分ER激动剂和拮抗剂的方法，包括ER分子内折叠系统的细胞，包括ER分子内折叠系统的转基因动物，融合蛋白和 喜欢。

公开(公告)号：US08173791B2

公开(公告)日：2012-05-08

申请号：US13070665

申请日：2011-03-24

Applicant: Sanjiv S. Gambhir ,  Andreas M. Loening ,  Anna M. Wu

Inventor： Sanjiv S. Gambhir ,  Andreas M. Loening ,  Anna M. Wu

IPC: C07H21/04 ,  C12N15/00 ,  C12N9/02

CPC classification number: C12Q1/66

Abstract: Briefly described, embodiments of this disclosure include polynucleotides that encode mutant Cnidarian luciferases that exhibit modulated properties as compared to the corresponding wild-type luciferases, and the modulated properties include at least one of: modulated stability; enhanced light output; and modulated emission maximum. Embodiments of the present disclosure also include polypeptides or fragments thereof encoded by the polynucleotides, constructs including the polynucleotide, expression cassettes, cells, methods of producing the polynucleotides and polypeptides, antibodies, transgenic cells and/or animals, kits, and the like.

Abstract translation: 简要描述，本公开的实施方案包括编码与相应的野生型萤光素酶相比表现出调节特性的突变型雀巢萤光素酶的多核苷酸，并且调节的性质包括以下至少一种：调节的稳定性; 增强光输出; 并调制发射最大值。 本公开的实施方案还包括由多核苷酸编码的多肽或其片段，包括多核苷酸的构建体，表达盒，细胞，产生多核苷酸和多肽的方法，抗体，转基因细胞和/或动物，试剂盒等。

公开(公告)号：US20100135912A1

公开(公告)日：2010-06-03

申请号：US12627299

申请日：2009-11-30

Applicant: Sanjiv S. Gambhir ,  Michael Benoit ,  A. C. Matin ,  Yoram Barak ,  Shay Keren ,  Dirk Mayer

Inventor： Sanjiv S. Gambhir ,  Michael Benoit ,  A. C. Matin ,  Yoram Barak ,  Shay Keren ,  Dirk Mayer

IPC: A61K49/08 ,  C12N1/20

CPC classification number: A61K49/1896 ,  C12N1/20 ,  C12P3/00 ,  C12R1/01

Abstract: Magnetic resonance imaging (MRI) is enhanced by contrast agents such as superparamagnetic iron-oxide (SPIO) particles that resemble magnetite particles produced by magnetotactic bacteria. Magnetospirillum magneticum AMB-1 produces positive MRI contrast when generating ultrasmall magnetite particles (10-40 nm diameter). Positive MRI contrast permits clearer distinction from image voids compared to negative contrast. T1-weighted MRI showed that such bacteria increased positive contrast 2.2-fold (p

Abstract translation: 磁共振成像（MRI）通过造影剂增强，如超顺磁性氧化铁（SPIO）颗粒，类似于由磁能细菌产生的磁铁矿颗粒。 当产生超小磁铁矿颗粒（直径为10-40nm）时，Magnetospirillum magneticum AMB-1产生正的MRI对比度。 与负面对比相比，正面MRI对比可以更清晰地区分图像空洞。 T1加权MRI显示，在小鼠肿瘤异种移植物中肿瘤内注射后，这种细菌体外增加了2.2倍（p <0.02）和2.0倍（p <0.02）。 静脉输送后，Magnetospirillum磁性AMB-1靶向肿瘤并产生增加的阳性MRI对比（1.4倍; p <0.01）。 通过活细胞计数，放射性标记的AMB-1的microPET成像和肿瘤切片的普鲁士蓝染色显示AMB-1肿瘤靶向。 因此，磁性细菌提供了通过MRI改善癌症诊断和监测治疗反应的工具。

公开(公告)号：US20100047813A1

公开(公告)日：2010-02-25

申请号：US12365984

申请日：2009-02-05

Applicant: Sanjiv S. Gambhir ,  Abhijit De

Inventor： Sanjiv S. Gambhir ,  Abhijit De

IPC: G01N33/53

CPC classification number: G01N33/542 ,  C12Q1/66

Abstract: Briefly described, embodiments of this disclosure include bioluminescence resonance energy transfer (BRET) systems, methods of detecting a protein-protein interaction, noninvasive methods for detecting the interaction of a first protein with a second protein within a living animal, methods to determine the efficacy of a test compound administered to modulate the interaction of a first protein with a second protein in a living animal, BRET vectors, kits relating to each of the above, transgenic cell or progeny thereof and/or animals relating to each of the above, and the like.

Abstract translation: 简要描述，本公开的实施方案包括生物发光共振能量转移（BRET）系统，检测蛋白质 - 蛋白质相互作用的方法，用于检测活体动物中第一种蛋白质与第二种蛋白质的相互作用的非侵入性方法，确定功效的方法 施用以调节活体动物中第一蛋白质与第二蛋白质的相互作用的测试化合物，BRET载体，与上述每种相关的试剂盒，转基因细胞或其后代和/或与上述每种相关的动物，和/或 类似。

公开(公告)号：US20090044286A1

公开(公告)日：2009-02-12

申请号：US11805460

申请日：2007-05-23

Applicant: Sanjiv S. Gambhir ,  Ramasamy Paulmurugan

Inventor： Sanjiv S. Gambhir ,  Ramasamy Paulmurugan

IPC: A01K67/027 ,  C07K14/00 ,  C12N9/02 ,  C12Q1/66 ,  C12N5/06

CPC classification number: C07K14/721 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/0331 ,  G01N33/542 ,  G01N2333/723

Abstract: Briefly described, embodiments of this disclosure include estrogen receptor (ER) intramolecular folding systems, methods of detecting ER ligands and distinguishing between ER agonists and antagonists, cells including ER intramolecular folding systems, transgenic animals including ER intramolecular folding systems, fusion proteins, and the like.

Abstract translation: 简要描述，本公开的实施方案包括雌激素受体（ER）分子内折叠系统，检测ER配体和区分ER激动剂和拮抗剂的方法，包括ER分子内折叠系统的细胞，包括ER分子内折叠系统的转基因动物，融合蛋白和 喜欢。

公开(公告)号：US20090004105A1

公开(公告)日：2009-01-01

申请号：US12215136

申请日：2008-06-25

Applicant: Zhen Cheng ,  Sanjiv S. Gambhir

Inventor： Zhen Cheng ,  Sanjiv S. Gambhir

IPC: A61K51/00 ,  G01N1/30

CPC classification number: A61K51/1096 ,  G01N1/30

Abstract: Embodiments of the present disclosure provide for: methods for imaging MMP-2 positive tissue; methods of diagnosing the presence of one or more of MMP-2 positive precancerous cells, MMP-2 positive cancerous cells, MMP-2 positive tumor cells, and MMP-2 positive diseases in a tissue; method of monitoring the progress of one or more of MMP-2 positive precancerous cells, MMP-2 positive cancerous cells, MMP-2 positive tumor cells, and MMP-2 positive diseases in a tissue; pharmaceutical compositions for imaging one or more of MMP-2 positive precancerous cells, MMP-2 positive cancerous cells, MMP-2 positive tumor cells, and MMP-2 positive diseases; compositions; kits; and the like.

Abstract translation: 本公开的实施方案提供：用于成像MMP-2阳性组织的方法; 在组织中诊断MMP-2阳性癌前细胞，MMP-2阳性癌细胞，MMP-2阳性肿瘤细胞和MMP-2阳性疾病的存在的方法; 监测组织中MMP-2阳性癌前细胞，MMP-2阳性癌细胞，MMP-2阳性肿瘤细胞和MMP-2阳性疾病中一种或多种的进展的方法; 用于成像MMP-2阳性癌前细胞，MMP-2阳性癌细胞，MMP-2阳性肿瘤细胞和MMP-2阳性疾病中的一种或多种的药物组合物; 组成; 工具包 等等。

公开(公告)号：US20200009376A1

公开(公告)日：2020-01-09

申请号：US16502255

申请日：2019-07-03

Applicant: Edwin CHANG ,  Chirag B. PATEL ,  Sanjiv S. GAMBHIR ,  Tali VOLOSHIN-SELA ,  Yaara PORAT ,  Moshe GILADI

Inventor： Edwin CHANG ,  Chirag B. PATEL ,  Sanjiv S. GAMBHIR ,  Tali VOLOSHIN-SELA ,  Yaara PORAT ,  Moshe GILADI

IPC: A61N1/32

Abstract: Certain substances (e.g., large molecules) that ordinarily cannot traverse the cell membrane of cells can be introduced into cells by applying an alternating electric field to the cell for a period of time, wherein the frequency of the alternating electric field is selected so that application of the alternating electric field increases permeability of the cell membrane. Once the permeability of the cell membrane has been increased, the substance is able to cross the cell membrane. This approach is particularly useful in the context of cancer cells (e.g., glioblastoma).

公开(公告)号：US08574547B2

公开(公告)日：2013-11-05

申请号：US12595525

申请日：2008-04-10

Applicant: Sanjiv S. Gambhir ,  Levi Jelena ,  Shay Keren

Inventor： Sanjiv S. Gambhir ,  Levi Jelena ,  Shay Keren

IPC: A61K49/00 ,  A61B5/00 ,  A61B8/00 ,  A61B10/00

CPC classification number: G01N29/2418 ,  A61B5/0059 ,  A61B5/0095 ,  G01N29/0654 ,  G01N33/573 ,  G01N33/583 ,  G01N2291/014 ,  G01N2291/015 ,  G01N2291/0255

Abstract: Embodiments of the present disclosure provide for photoacoustic probes, methods of determining the presence and location of a specific target, methods of determining the presence and location of an enzyme, methods of determining the presence and location of a specific target and an enzyme, and the like.

Abstract translation: 本公开的实施方案提供光声探测器，确定特定靶标的存在和位置的方法，确定酶的存在和位置的方法，确定特异性靶标和酶的存在和位置的方法，以及 喜欢。