知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

21 条记录 (耗时 0.027 秒)

    Method of preparing murine monoclonal antibodies against cell-free products of activated human T-lymphocytes
    9.
    发明授权
    Method of preparing murine monoclonal antibodies against cell-free products of activated human T-lymphocytes 失效
    制备针对活化的人T淋巴细胞的无细胞产物的鼠单克隆抗体的方法

    公开(公告)号：US4596774A

    公开(公告)日：1986-06-24

    申请号：US547941

    申请日：1983-11-02

    申请人： Tse-Wen Chang ,  Nancy T. Chang

    发明人： Tse-Wen Chang ,  Nancy T. Chang

    IPC分类号： C07K16/18 ,  C12P21/08 ,  C12P21/00 ,  C12N5/00 ,  C12N5/02 ,  C12R1/91

    CPC分类号： C07K16/18 ,  C07K2317/74 ,  Y10S530/806

    摘要： A method of preparing simultaneously monoclonal antibodies specific for different cell-free products of activated human T lymphocytes is disclosed. Human T cells are activated in a medium supplemented with mouse serum rather than conventional calf serum. A supernatant prepared from the activated T cells is used to immunize mice. The dominant immunogens in the supernatant are the cell-free products of human T lymphocytes. The yield of hybrid cells which produce products reactive with cell-free products of human T lymphocytes is enhanced by injecting the immunized mice with a supernatant from mitogen-activated murine splenocytes. In addition, a novel radioimmunoadsorbent assay for screening hybrids to detect production of monoclonal antibodies reactive with cell-free products of human T lymphocytes is disclosed.

    摘要翻译： 公开了同时制备对活化的人T淋巴细胞的不同无细胞产物特异的单克隆抗体的方法。 人T细胞在补充有小鼠血清而不是常规小牛血清的培养基中活化。 使用从活化的T细胞制备的上清液来免疫小鼠。 上清液中的主要免疫原是人T淋巴细胞的无细胞产物。 通过从有丝分裂原激活的小鼠脾细胞中注射免疫小鼠来增强产生与人T淋巴细胞无细胞产物反应的产物的杂交细胞的产量。 此外，公开了一种用于筛选杂交体以检测与人T淋巴细胞的无细胞产物反应的单克隆抗体的产生的新型放射免疫吸附测定法。