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15 条记录 (耗时 0.015 秒)

    Method for producing protein
    1.
    发明授权
    Method for producing protein 有权

    公开(公告)号：US10442843B2

    公开(公告)日：2019-10-15

    申请号：US15685053

    申请日：2017-08-24

    申请人： NIPPI, INCORPORATED

    发明人： Tomonori Ueno ,  Yuki Taga ,  Kiyoko Goto ,  Yuko Kaku

    IPC分类号： C07K14/47 ,  C12N15/85 ,  C12P21/00 ,  C12P21/02 ,  C12N15/67 ,  C07K14/705

    摘要： It has been believed that promoting the assembly of polysomes composed of many ribosomes attached to mRNA is very effective for highly efficient protein synthesis. However, the mechanism for p180 protein's capability of promoting polysome formation has been yet to be elucidated.The inventors of the present application newly discovered SF3b4 protein as a protein that specifically interacts with the coiled-coil domain of p180 protein, a responsible region for its capability of promoting polysome formation, and which is capable of promoting mRNA localization to an endoplasmic reticulum (ER). The inventors also found that, in cells capable of highly expressing both p180 protein and a protein promoting mRNA localization to an endoplasmic reticulum (ER) (e.g., SF3b4 protein), the mRNA localization to the endoplasmic reticulum can be significantly elevated so that the secretory capacity in cultured cells can be enhanced. Further, the inventors demonstrated that when a particular nucleotide sequence is inserted into an expression plasmid, SF3b4 protein exhibiting protein expression enhancing ability can be localized onto the endoplasmic reticulum membrane, and the mRNA distribution in polysomes can be shifted towards heavier fractions, whereby the secretory capacity in cells can be enhanced.

    Method for producing protein
    3.
    发明授权
    Method for producing protein 有权

    公开(公告)号：US09884897B2

    公开(公告)日：2018-02-06

    申请号：US14780313

    申请日：2014-03-26

    申请人： NIPPI, INCORPORATED

    发明人： Tomonori Ueno ,  Yuki Taga ,  Kiyoko Goto ,  Yuko Kaku

    IPC分类号： C07K14/47 ,  C12P21/02 ,  C12N15/67 ,  C07K14/705 ,  C12N15/85 ,  C12P21/00

    CPC分类号： C07K14/47 ,  C07K14/70553 ,  C12N15/67 ,  C12N15/85 ,  C12P21/00 ,  C12P21/02

    摘要： It has been believed that promoting the assembly of polysomes composed of many ribosomes attached to mRNA is very effective for highly efficient protein synthesis. However, the mechanism for p180 protein's capability of promoting polysome formation has been yet to be elucidated.The inventors of the present application newly discovered SF3b4 protein as a protein that specifically interacts with the coiled-coil domain of p180 protein, a responsible region for its capability of promoting polysome formation, and which is capable of promoting mRNA localization to an endoplasmic reticulum (ER). The inventors also found that, in cells capable of highly expressing both p180 protein and a protein promoting mRNA localization to an endoplasmic reticulum (ER) (e.g., SF3b4 protein), the mRNA localization to the endoplasmic reticulum can be significantly elevated so that the secretory capacity in cultured cells can be enhanced. Further, the inventors demonstrated that when a particular nucleotide sequence is inserted into an expression plasmid, SF3b4 protein exhibiting protein expression enhancing ability can be localized onto the endoplasmic reticulum membrane, and the mRNA distribution in polysomes can be shifted towards heavier fractions, whereby the secretory capacity in cells can be enhanced.

    Collagen Peptide Composition Production Method, DPP-4 Inhibitor, and Antihyperglycemic Agent
    4.
    发明申请
    Collagen Peptide Composition Production Method, DPP-4 Inhibitor, and Antihyperglycemic Agent 有权
    胶原肽组合物制备方法，DPP-4抑制剂和抗高血糖剂

    公开(公告)号：US20150182580A1

    公开(公告)日：2015-07-02

    申请号：US14416524

    申请日：2013-07-23

    申请人： Nippi, Incorporated

    发明人： Yuki Taga ,  Masashi Kusubata ,  Satoshi Suzuki ,  Osamu Hayashida ,  Yoh-ichi Koyama ,  Shunji Hattori

    IPC分类号： A61K38/01 ,  C12P21/06

    CPC分类号： A61K38/014 ,  A61K38/39 ,  C07K14/78 ,  C12P21/06

    摘要： Provided are methods of producing a novel collagen peptide composition, and a DPP-4 inhibitor and antihyperglycemics that comprise the above-mentioned collagen peptide composition. Ginger rhizome-derived enzymes are added to and break down a collagen and/or gelatin solution to generate peptide compositions comprising peptides represented by X-Hyp-Gly (wherein X represents an amino acid residue other than Gly, Hyp, and Pro). The thus obtained collagen peptide composition has a high DPP-4 inhibitory activity and an excellent antihyperglycemic effect.

    摘要翻译： 提供了生产新型胶原肽组合物的方法和包含上述胶原肽组合物的DPP-4抑制剂和抗高血糖药物的方法。 将姜根茎衍生的酶加入并分解胶原蛋白和/或明胶溶液以产生包含由X-Hyp-Gly（其中X表示除Gly，Hyp和Pro之外的氨基酸残基）表示的肽的肽组合物。 由此获得的胶原肽组合物具有高的DPP-4抑制活性和优异的抗高血糖作用。

    COLLAGEN STRUCTURE, AND METHOD FOR PRODUCING COLLAGEN STRUCTURE
    9.
    发明申请
    COLLAGEN STRUCTURE, AND METHOD FOR PRODUCING COLLAGEN STRUCTURE 审中-公开
    胶原结构和生产胶原结构的方法

    公开(公告)号：US20150004414A1

    公开(公告)日：2015-01-01

    申请号：US14371845

    申请日：2013-01-11

    申请人： NIPPI, INCORPORATED

    发明人： Takayuki Ogura ,  Keisuke Tanaka ,  Yasuhiro Ohba ,  Shunji Hattori

    IPC分类号： A61L31/14 ,  A61L31/04

    CPC分类号： A61L31/14 ,  A61K8/027 ,  A61K8/65 ,  A61K9/7007 ,  A61K47/42 ,  A61K2800/412 ,  A61K2800/91 ,  A61L27/24 ,  A61L31/044 ,  A61L2300/414 ,  A61L2300/418 ,  A61L2300/42 ,  A61L2300/80 ,  A61Q19/08 ,  Y10T428/298

    摘要： Provided is a collagen structure characterized by: comprising collagen fibers of 1 to 5 μm in average diameter; and has a water content of 0 to 15 (w/w)% and a collagen density of 50 to 800 mg/cm3. After generating collagen fibers by neutralizing an acidic collagen solution, the resulting solution is subjected to filtration or the like to form crude collagen fibers having a collagen concentration of 12 to 50 (w/v)%. The thus obtained crude collagen fibers are molded into a prescribed shape and then dried, thereby the collagen structure can be produced. Since the collagen structure is produced using, as raw material, collagen fibers that are formed by association of collagen molecules, the collagen structure has excellent cell infiltration property. Further, since the collagen density of the collagen structure is equivalent to that of in vivo collagen tissue, the collagen structure exhibits excellent tissue regeneration capacity when filled into a defective part in vivo. Therefore, the collagen structure can be preferably used as an artificial material for regenerative medicine and the like.

    摘要翻译： 提供一种胶原结构，其特征在于：包含平均直径为1〜5μm的胶原纤维; 水分含量为0〜15（w / w）％，胶原密度为50〜800mg / cm3。 通过中和酸性胶原溶液来生成胶原纤维后，将所得溶液进行过滤等以形成胶原浓度为12〜50（w / v）％的粗胶原纤维。 将由此获得的粗胶原纤维模制成规定形状，然后干燥，从而可以制备胶原蛋白结构。 由于胶原结构以胶原分子缔合形成的胶原纤维为原料，因此胶原结构具有优异的细胞渗透性。 此外，由于胶原结构的胶原密度与体内胶原组织的胶原密度相同，所以当在体内填充缺损部位时，胶原结构显示优异的组织再生能力。 因此，胶原结构优选用作再生医药等的人造材料。