公开(公告)号：US10047353B2

公开(公告)日：2018-08-14

申请号：US15123986

申请日：2015-03-06

申请人： Nippi, Incorporated ,  The University of Tokyo

发明人： Naoko Teramura ,  Katsumasa Iijima ,  Osamu Hayashida ,  Keisuke Tanaka ,  Shunji Hattori ,  Teru Okitsu ,  Shoji Takeuchi

IPC分类号： C12N9/52

摘要： Recombinant collagenases with a stable specific activity and enzyme agents for cell and tissue dissociation such a recombinant are provided. The recombinant collagenase is derived from Grimontia hollisae-derived collagenase is characterized by having, from the N terminus to the C terminus, a collagenase catalytic domain, a linker region sequence, and a prepeptidase C terminal domain, which Grimontia hollisae-derived recombinant collagenase does not comprise at least the prepeptidase C terminal domain. The obtained recombinant collagenase has a high and stable specific activity.

公开(公告)号：US20170218352A1

公开(公告)日：2017-08-03

申请号：US15123986

申请日：2015-03-06

申请人： Nippi, Incorporated ,  The University of Tokyo

发明人： Naoko Teramura ,  Katsumasa Iijima ,  Osamu Hayashida ,  Keisuke Tanaka ,  Shunji Hattori ,  Teru Okitsu ,  Shoji Takeuchi

IPC分类号： C12N9/52

CPC分类号： C12N9/52 ,  C12Y304/24007

摘要： Recombinant collagenases with a stable specific activity and enzyme agents for cell and tissue dissociation such a recombinant are provided. The recombinant collagenase is derived from Grimontia hollisae-derived collagenase is characterized by having, from the N terminus to the C terminus, a collagenase catalytic domain, a linker region sequence, and a prepeptidase C terminal domain, which Grimontia hollisae-derived recombinant collagenase does not comprise at least the prepeptidase C terminal domain. The obtained recombinant collagenase has a high and stable specific activity.

公开(公告)号：US20230020499A1

公开(公告)日：2023-01-19

申请号：US17787161

申请日：2020-12-24

申请人： The University Of Tokyo

发明人： Shoji Takeuchi ,  Yuya Morimoto ,  Byeongwook Jo ,  Minghao Nie ,  Ai Tajima

IPC分类号： C12N5/00 ,  C12N5/071 ,  C12N5/077

摘要： A method of producing a 3D tissue composite, comprising: a preparation step in which a multiple number of sheet-shaped first structures containing first cells are prepared, wherein at least one of the multiple number of first structures holds a second structure containing second cells; a stacking step in which the multiple number of first structures are stacked to form a 3D composite; and a culturing step in which the 3D composite is cultured to form a 3D tissue composite containing first tissues formed from the first cells and second tissues formed from the second cells.

公开(公告)号：US09399789B2

公开(公告)日：2016-07-26

申请号：US13741825

申请日：2013-01-15

申请人： FUJIKURA LTD. ,  BIO ELECTRO-MECHANICAL AUTONOMOUS NANO SYSTEMS LABORATORY TECHNOLOGY RESEARCH ASSOCIATION ,  THE UNIVERSITY OF TOKYO

发明人： Osamu Nukaga ,  Satoshi Yamamoto ,  Kazuhito Tabata ,  Masakazu Sugiyama ,  Shoji Takeuchi

IPC分类号： C12M1/36 ,  C12M3/00 ,  C12Q1/24 ,  B23K26/00 ,  G01N33/487

CPC分类号： C12Q1/24 ,  B23K26/55 ,  G01N33/48728

摘要： Disclosed is a substrate (10A) for trapping a microorganism or cell (T), characterized by comprising a base (4) and having a space (2) into which a fluid (R) containing the microorganism or cell (T) is introduced and a microfine suction hole (1) through which the space (2) communicates with the outside of the base (4). The substrate is further characterized in that the space (2) has been formed in the base (4), and at least the portion of the base (4) which forms the microfine suction hole (1) is constituted of a single member.

摘要翻译： 公开了一种用于捕获微生物或细胞（T）的底物（10A），其特征在于包含碱（4）并具有空间（2），其中引入含有微生物或细胞（T）的流体（R） 微空气吸入孔（1），空间（2）通过该微孔与基座（4）的外部连通。 基板的特征还在于，在基座（4）上形成有空间（2），至少形成微细吸引孔（1）的基部（4）的一部分由单一构件构成。

公开(公告)号：US10221382B2

公开(公告)日：2019-03-05

申请号：US15312561

申请日：2015-05-20

申请人： THE UNIVERSITY OF TOKYO

发明人： Shoji Takeuchi ,  Hiroaki Onoe ,  Shigenori Miura

IPC分类号： C12M1/12 ,  B01D69/08 ,  B01D69/12 ,  B01D69/14 ,  B01D17/00

摘要： The present invention relates to a hollow microfiber comprising (1) one or more cell-adhesive layers having a cell-adhesive hydrogel, (2) an outer shell layer having a high-strength hydrogel that covers the outer periphery of the cell-adhesive layer that is positioned farthest from the center axis among the one or more cell-adhesive layers, and (3) a cell layer that covers the inner periphery of the cell-adhesive layer that is positioned closest to the center axis among the one or more cell-adhesive layers. The present invention also relates to a method of manufacturing the hollow microfiber and a kit for carrying out the manufacturing method.