知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

21 条记录 (耗时 0.018 秒)

    OLIGONUCLEOTIDE LIBRARY ENCODING RANDOMISED PEPTIDES
    3.
    发明申请
    OLIGONUCLEOTIDE LIBRARY ENCODING RANDOMISED PEPTIDES 审中-公开
    OLIGONUCLEOTIDE LIBRARY ENCODING RANDOMIZED PEPTIDES

    公开(公告)号：US20130130938A1

    公开(公告)日：2013-05-23

    申请号：US13744616

    申请日：2013-01-18

    申请人： Mohammed Ashraf ,  Marcus Daniel Hughes ,  Anna Victoria Hine

    发明人： Mohammed Ashraf ,  Marcus Daniel Hughes ,  Anna Victoria Hine

    IPC分类号： B01J19/00

    CPC分类号： B01J19/0046 ,  C12N15/66

    摘要： The invention relates to a method of producing an oligonucleotide library comprising a plurality of oligonucleotides, each oligonucleotide in the library having at least one predetermined position, a randomisation codon selected from a defined group of codons, the codons within said defined group coding for different amino acids. Vector, host cells containing such libraries and kits for the production of such libraries are also provided.

    摘要翻译： 本发明涉及生产包含多个寡核苷酸的寡核苷酸文库的方法，文库中的每个寡核苷酸具有至少一个预定位置，选自定义的密码子组的随机化密码子，所述定义的组中的密码子编码不同的氨基 酸。 还提供了载体，含有这种文库的宿主细胞和用于生产这种文库的试剂盒。

    METHOD FOR DETECTION OF CYANIDE IN WATER
    7.
    发明申请
    METHOD FOR DETECTION OF CYANIDE IN WATER 有权
    水中氰化物检测方法

    公开(公告)号：US20130203178A1

    公开(公告)日：2013-08-08

    申请号：US13827999

    申请日：2013-03-14

    申请人： MOHAMMED ASHRAF GONDAL ,  QASEM AHMED QASEM DRMOSH ,  Z.H. YAMANI ,  TAWFIK ABDO SALEH

    发明人： MOHAMMED ASHRAF GONDAL ,  QASEM AHMED QASEM DRMOSH ,  Z.H. YAMANI ,  TAWFIK ABDO SALEH

    IPC分类号： G01N27/30 ,  C01B15/047

    CPC分类号： G01N27/30 ,  C01B15/047 ,  G01N27/4166 ,  G01N33/182 ,  Y10S977/773 ,  Y10T436/172307

    摘要： The method for detection of cyanide in water is a method for the detection of a highly toxic pollutant, cyanide, in water using ZnO2 nanoparticles synthesized locally by an elegant Pulsed Laser Ablation technique. ZnO2 nanoparticles having a median size of 4 nm are synthesized from pure zinc metal target under UV laser irradiation in a 1-10% H2O2 environment in deionized water. The synthesized ZnO2 nanoparticles are suspended in dimethyl formamide in the presence of Nafion, and then ultrasonicated to create a homogenous suspension, which is used to prepare a thin film of ZnO2 nanoparticles on a metal electrode. The electrode is used for cyanide detection.

    摘要翻译： 氰化物在水中的检测方法是使用典型的脉冲激光烧蚀技术局部合成的ZnO 2纳米颗粒，检测水中高毒性污染物氰化物的方法。 中性粒径为4nm的ZnO 2纳米粒子在纯化学金属靶材中，在去离子水中，在1-10％H 2 O 2环境下，在UV激光照射下合成。 合成的ZnO 2纳米颗粒在Nafion存在下悬浮于二甲基甲酰胺中，然后进行超声波处理，产生均匀的悬浮液，用于在金属电极上制备ZnO2纳米粒子薄膜。 电极用于氰化物检测。

    Methods of producing dna and protein libraries
    8.
    发明申请
    Methods of producing dna and protein libraries 审中-公开
    生产dna和蛋白质文库的方法

    公开(公告)号：US20060269913A1

    公开(公告)日：2006-11-30

    申请号：US10518174

    申请日：2003-06-13

    申请人： Anna Hine ,  David Nagel ,  Marcus Hughes ,  Zhan-Ren Zhang ,  Mohammed Ashraf ,  Andrew Sutherland ,  Albert Santos

    发明人： Anna Hine ,  David Nagel ,  Marcus Hughes ,  Zhan-Ren Zhang ,  Mohammed Ashraf ,  Andrew Sutherland ,  Albert Santos

    IPC分类号： C40B30/06 ,  C40B40/08

    CPC分类号： C12N15/1093 ,  C12N15/67

    摘要： The present invention provides a method of producing a DNA library comprising a plurality of DNA sequences of interest, where each DNA sequence of interest has at least two predetermined positions, with at each predetermined position a codon (MAX) selected from a defined group for that position, the codons within a group coding for different amino acids. The method comprising the steps of:—(i) contacting so as to effect hybridisation (a) template DNA (A) comprising said at least two predetermined positions, said template DNA being fully randomised at said at least two predetermined positions (NNN), (b) for each predetermined position, a selection oligonucleotide pool, each selection oligonucleotide (B) within each pool comprising a codon (MAX) selected from the defined group for that predetermined position, and (c) at least one additional oligonucleotide sequence (E) comprising a region (E2) which is non-hybridisable to the template DNA, (ii) ligating the hybridised DNA sequences (B, E), (iii) denaturing the product of step (ii) so as to give a mixed population of said template DNA (A) and said DNA sequences of interest, and (iv) selectively amplifying the DNA sequences of interest. The additional oligonucleotide sequence (E) of step (i) is selected such that after step (ii) the non-hybridisable region (E2) is located externally of the template DNA (A) The invention also provides protein and DNA libraries which can be produced by the method of the invention.

    摘要翻译： 本发明提供了一种生产包含多个感兴趣的DNA序列的DNA文库的方法，其中每个目标DNA序列具有至少两个预定位置，在每个预定位置，选自所定义组的密码子（MAX） 位置，编码不同氨基酸的组内的密码子。 所述方法包括以下步骤： - （i）接触以便实现杂交（a）包含所述至少两个预定位置的模板DNA（A），所述模板DNA在所述至少两个预定位置（NNN）完全随机化， （b）对于每个预定位置，选择寡核苷酸库，每个库内的每个选择寡核苷酸（B）包含选自该预定位置的定义基团的密码子（MAX），和（c）至少一个另外的寡核苷酸序列（E ）包含与模板DNA不可杂交的区域（E 2），（ii）连接杂交的DNA序列（B，E），（iii）使步骤（ii）的产物变性以得到混合的群体 的所述模板DNA（A）和所述感兴趣的DNA序列，和（iv）选择性扩增目的DNA序列。 选择步骤（i）的另外的寡核苷酸序列（E）使得在步骤（ii）之后，不可混合区域（E 2）位于模板DNA外部（A）。本发明还提供蛋白质和DNA文库，其可以 通过本发明的方法生产。