公开(公告)号：US11536726B2

公开(公告)日：2022-12-27

申请号：US17043165

申请日：2019-04-01

申请人： Metabolon, Inc.

发明人： Lisa Ford ,  Kirk L. Pappan ,  Tiffany A. Freed ,  Kelsey M. Eason

IPC分类号： G01N33/68 ,  G01N30/72 ,  G01N33/49 ,  H01J49/00 ,  H01J49/04 ,  G01N33/483 ,  G01N33/52 ,  G01N30/02 ,  G01N30/04

摘要： A method for determining in a sample, by mass spectrometry, the amount of one or more analytes selected from the group consisting of 12,13-DiHOME, 3-hydroxybutyrate (BHBA), 3-hydroxyoctanoate, 3-methylglutarylcarnitine, 3-ureidopropionate, 7-alpha-hydroxy-4-cholesten-3-one (7-Hoca), citrate, fucose, fumarate, gamma-tocopherol, glutamate, glutarate, glycerol, glycochenodeoxycholate, glycocholate, hypoxanthine, maleate, malonate, mannose, orotate, 2,3-pyrdinedicarboxylate, ribose, serine, taurine, taurochenodeoxycholate, taurocholate, palmitoleate, linolenate, xanthine, xylitol, and combinations thereof is described. The method comprises subjecting the sample to an ionization source under conditions suitable to produce one or more ions detectable by mass spectrometry from each of the one or more analytes; measuring, by mass spectrometry, the amount of the one or more ions from each of the one or more analytes; and using the measured amount to determine the amount of each of the one or more analytes in the sample.

公开(公告)号：US11527306B2

公开(公告)日：2022-12-13

申请号：US16647883

申请日：2018-10-04

申请人： Metabolon, Inc.

发明人： Adam D. Kennedy

IPC分类号： G16B40/10 ,  G16C20/20 ,  H01J49/00

摘要： A method of assessing the analytical performance of a biochemical measured using a multi-analyte assay is described. The method includes analytically validating a measurement of the level of a first biochemical in a sample, wherein the first biochemical has been previously analytically validated for three or more analytical validation conditions; measuring the level of a second biochemical in a sample, wherein the second biochemical is structurally or biochemically related to the first biochemical; and comparing validation parameters of the first biochemical with validation parameters of the second biochemical to determine whether the performance of the second biochemical is acceptable based on the comparison results.

公开(公告)号：US20220221433A1

公开(公告)日：2022-07-14

申请号：US17611639

申请日：2020-05-08

申请人： Metabolon, Inc.

发明人： Richard J. Robinson ,  Anne M. EVANS ,  Elizaveta FREINKMAN

IPC分类号： G01N30/72 ,  G01N30/06

摘要： A method for determining in a sample, by liquid chromatography/mass spectrometry, the presence, absence, or amount of one or more or a plurality of analytes is described. The method comprises subjecting the sample to an ionization source under conditions suitable to produce one or more ions detectable by mass spectrometry from each of the one or more of the analytes; measuring, by liquid chromatography/mass spectrometry, the amount of the one or more ions from each of the one or more analytes; and using the measured amount of the one or more ions to determine the presence, absence, or amount of each of the one or more or plurality of analytes in the sample.

公开(公告)号：US20200166519A1

公开(公告)日：2020-05-28

申请号：US16305078

申请日：2017-05-26

申请人： Metabolon, Inc.

发明人： Lisa FORD ,  Qibo ZHANG ,  Klaus Peter ADAM

IPC分类号： G01N33/68 ,  G01N30/72

摘要： A method for determining the amount of one or more analytes in a sample by mass spectrometry is described. The one or more analytes are selected from the group consisting of alpha-hydroxybutyrate (2-HB), linoleoyl LPC (LGPC), oleic acid, 3-hydroxybutyrate (3-HB), 4-methyl-2-oxopentanoate (4-MOP), pantothenate, and serine. The method includes a) subjecting the sample to an ionization source under conditions suitable to produce one or more ions detectable by mass spectrometry from each of the one or more of the analytes, wherein the analytes are not derivatized prior to ionization; b) measuring, by tandem mass spectrometry, the amount of the one or more ions from each of the one or more analytes; and c) using the measured amount of the one or more ions to determine the amount of each of the one or more analytes in the sample.

公开(公告)号：US20190152907A1

公开(公告)日：2019-05-23

申请号：US16321135

申请日：2017-07-27

申请人： Metabolon, Inc.

发明人： Qibo Zhang ,  Anne M. Evans

IPC分类号： C07D207/16 ,  C07B59/00 ,  G01N33/53

摘要： The present invention provides a compound of formula (I) or a salt thereof and their use as a biomarker in assessing or monitoring kidney function in a subject, determining predisposition to developing reduced kidney function, classifying a subject according to level of kidney function, diagnosing or monitoring chronic kidney disease. Compositions and method of making of the compound of formula (I) are also described.

公开(公告)号：US20190120855A1

公开(公告)日：2019-04-25

申请号：US15955883

申请日：2018-04-18

申请人： Metabolon, Inc. ,  The Cleveland Clinic Foundation

发明人： Bruce J. McCreedy ,  Alvin Berger ,  YunFu Hu ,  Satish C. Kalhan

IPC分类号： G01N33/68 ,  H01J49/00

摘要： The present invention provides various biomarkers of fatty liver disease, including steatosis and steatohepatitis. The present invention also provides various methods of using the biomarkers, including methods for diagnosis of fatty liver disease, methods of determining predisposition to fatty liver disease, methods of monitoring progression/regression of fatty liver disease, methods of assessing efficacy of compositions for treating fatty liver disease, methods of screening compositions for activity in modulating biomarkers of fatty liver disease, methods of treating fatty liver disease, as well as other methods based on biomarkers of fatty liver disease.

公开(公告)号：US20170192023A1

公开(公告)日：2017-07-06

申请号：US15251579

申请日：2016-08-30

申请人： Metabolon, Inc.

发明人： Steven Watkins

IPC分类号： G01N33/92 ,  G01N33/58

CPC分类号： G01N33/92 ,  G01N33/532 ,  G01N33/58 ,  G01N2405/00 ,  G01N2458/15 ,  G01N2560/00

摘要： Methods are provided for synthesizing mixtures of lipids that are representative of the structural diversity of the lipids present in samples of interest. The complex mixtures of lipids produced according to the methods of the present disclosure can be used as internal standards for detecting and quantifying the lipids in samples of interest. Kits including the internal standards and instructions for their use in the detection and quantification of lipids in samples of interest are also provided.

公开(公告)号：US20140201249A1

公开(公告)日：2014-07-17

申请号：US13800010

申请日：2013-03-13

申请人： METABOLON, INC.

发明人： Corey Donald DeHaven ,  Robnet Thornhill Kerns

IPC分类号： G06F17/30

CPC分类号： G16B5/00

摘要： A method is provided for analyzing metabolomics data for a plurality of metabolites. Each metabolite is assigned to a node. Nodes are connected according to a defined relationship between corresponding metabolites to form a nodal network. The nodal network is graphically displayed such that at least a portion of the nodes and the relationships therebetween are visible in a single view. An apparatus comprising a processor configured to control the apparatus to analyze metabolomics data for a plurality of metabolites, as well as a computer program product comprising at least one non-transitory computer readable storage medium having computer program code stored thereon, the computer program code being configured to analyze metabolomics data for a plurality of metabolites, are also provided.

摘要翻译： 提供了一种用于分析多种代谢物的代谢组学数据的方法。 每个代谢物被分配到一个节点。 节点根据相应代谢物之间的定义关系连接，形成节点网络。 节点网络被图形显示，使得节点的至少一部分及其间的关系在单个视图中是可见的。 一种包括处理器的装置，其被配置为控制所述装置分析多个代谢物的代谢组学数据，以及包括其上存储有计算机程序代码的至少一个非暂时计算机可读存储介质的计算机程序产品，所述计算机程序代码 被配置为分析多种代谢物的代谢组学数据。

公开(公告)号：US20040146853A1

公开(公告)日：2004-07-29

申请号：US10757616

申请日：2004-01-13

申请人： METABOLON, INC.

发明人： Rima Kaddurah-Daouk ,  Bruce Kristal

IPC分类号： G01N033/48

CPC分类号： G01N33/6803 ,  G01N33/5091 ,  G01N2800/52 ,  G06F19/00 ,  Y02A90/26

摘要： The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed.

摘要翻译： 比较细胞的小分子轮廓以鉴定在改变状态下调节的小分子。 还讨论了细胞小分子文库，鉴定组织来源的方法，用于治疗遗传和非遗传性疾病的方法以及预测药物功效的方法。

公开(公告)号：US11680042B2

公开(公告)日：2023-06-20

申请号：US16650429

申请日：2018-09-27

申请人： Metabolon, Inc.

发明人： Qibo Zhang

IPC分类号： C07C323/54 ,  C07C319/18 ,  C07K16/44 ,  G01N30/72

CPC分类号： C07C323/54 ,  C07C319/18 ,  C07K16/44 ,  G01N30/7233

摘要： The present invention provides a compound of formula (I), formula (II), formula (III), (IV) or a salt thereof, compositions and methods of making the compound, methods and reagents for measuring the compound, and kits using the same. The use of a compound of formula (I), formula (II), formula (III), or formula (IV) for assessing or monitoring kidney function in a subject, determining predisposition to developing reduced kidney function, classifying a subject according to level of kidney function, and diagnosing or monitoring chronic kidney disease is also described.