知搜-全球专利检索

  1. Login
  2. Sign Up

Search Results

25 records (took 0.033 seconds)

    Method and Apparatus for Pipette Tip Columns
    2.
    发明申请
    Method and Apparatus for Pipette Tip Columns 有权
    移液管柱的方法和装置

    公开(公告)号：US20160061697A1

    公开(公告)日：2016-03-03

    申请号：US14846825

    申请日：2015-09-07

    Applicant: Douglas T. Gjerde ,  Lee Hoang ,  Chris Suh ,  Mark Abel

    Inventor： Douglas T. Gjerde ,  Lee Hoang ,  Chris Suh ,  Mark Abel

    IPC: G01N1/34 ,  B01L3/00 ,  B01L3/02

    CPC classification number: G01N1/34 ,  B01L3/021 ,  B01L3/0237 ,  B01L3/0275 ,  B01L3/5085 ,  B01L2200/025 ,  B01L2200/0631 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2400/0487 ,  G01N1/40 ,  Y10T436/255

    Abstract: An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The pipette is hand-held when transferring between positions, e.g., between operation steps that take place in different rows of wells.The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation.In some embodiments, cells are purified from biological samples.

    Abstract translation: 开发了一种使用带有移液管尖端柱的移液器的装置和方法，其中移液管在移液管尖端柱插入微量培养板的孔中进行操作。 在该配置中，移液管是自由站立的，并且基本上垂直于微孔板。 移液器在位置之间转移时是手持式的，例如在不同排井中发生的操作步骤之间。 移液管尖端柱的开放的下端大致在板孔内部居中。 柱和板被设计成使得移液管尖端柱的开口下端与板孔中的液体接触，但是柱不会在井底密封，从而防止流入和流出柱。 移液器包含适当的固件和软件，用于控制移液管尖端柱操作的所有步骤的流量。 在一些实施方案中，细胞从生物样品中纯化。

    Methods and devices for nucleic acid purification
    3.
    发明授权
    Methods and devices for nucleic acid purification 有权
    用于核酸纯化的方法和装置

    公开(公告)号：US08921539B2

    公开(公告)日：2014-12-30

    申请号：US13434656

    申请日：2012-03-29

    Applicant: Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

    Inventor： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

    IPC: C07H21/00

    CPC classification number: C12N15/101

    Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit.

    Abstract translation: 本发明提供了用于纯化核酸例如来自含有细胞碎片的未澄清细胞裂解物的质粒的移液管尖塔和自动化方法，以及用于制备和使用这些柱的方法。 柱通常包括位于移液管末端柱中的介质床，位于底部玻璃料上方并具有可选的顶部玻璃料。

    Purification of nucleic acids
    4.
    发明授权
    Purification of nucleic acids 有权

    公开(公告)号：US08664378B2

    公开(公告)日：2014-03-04

    申请号：US13250040

    申请日：2011-09-30

    Applicant: Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

    Inventor： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

    IPC: C07H21/00

    Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.

    Purification of Nucleic acids
    5.
    发明申请
    Purification of Nucleic acids 有权
    核酸的纯化

    公开(公告)号：US20120083598A1

    公开(公告)日：2012-04-05

    申请号：US13250040

    申请日：2011-09-30

    Applicant: Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

    Inventor： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde

    IPC: C07H21/00

    CPC classification number: C12N15/1017

    Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.

    Abstract translation: 本发明解决了在生长培养基存在下从细胞中分离核酸的问题。 本发明特别可用于分离染色体外复制子如质粒。 细胞在其生长培养基的存在下裂解，并使用移液管末端柱分离核酸。 可以使用液体处理机器人同时分离多个样品中的核酸而不需要人为干预。

    METHODS AND DEVICES FOR NUCLEIC ACID PURIFICATION
    7.
    发明申请
    METHODS AND DEVICES FOR NUCLEIC ACID PURIFICATION 审中-公开
    用于核酸净化的方法和装置

    公开(公告)号：US20170029809A1

    公开(公告)日：2017-02-02

    申请号：US15290847

    申请日：2016-10-11

    Applicant: Chris Suh ,  Carrie Loan Kim Huynh ,  Lee Hoang ,  Douglas T. Gjerde ,  Jonathan Michael Grambow

    Inventor： Chris Suh ,  Carrie Loan Kim Huynh ,  Lee Hoang ,  Douglas T. Gjerde ,  Jonathan Michael Grambow

    IPC: C12N15/10 ,  B01D37/03 ,  B01D15/10

    Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.

    Abstract translation: 本发明提供用于纯化包括质粒在内的核酸的移液管末端柱和自动化方法。 核酸可以从含有细胞碎片的未澄清，澄清或部分澄清的细胞裂解物中纯化。 柱通常包括位于移液管末端柱中的介质床，位于底部玻璃料上方并具有可选的顶部玻璃料。 质粒制备标准包括miniprep，midiprep，maxiprep，megaprep和gigaprep。

    METHODS AND DEVICES FOR NUCLEIC ACID PURIFICATION
    8.
    发明申请
    METHODS AND DEVICES FOR NUCLEIC ACID PURIFICATION 审中-公开
    用于核酸净化的方法和装置

    公开(公告)号：US20150119563A1

    公开(公告)日：2015-04-30

    申请号：US14585070

    申请日：2014-12-29

    Applicant: Chris Suh ,  Carrie Loan Kim Huynh ,  Lee Hoang ,  Douglas T. Gjerde

    Inventor： Chris Suh ,  Carrie Loan Kim Huynh ,  Lee Hoang ,  Douglas T. Gjerde

    IPC: C12N15/10

    CPC classification number: C12N15/1003 ,  C12N15/101

    Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit.

    Abstract translation: 本发明提供了用于纯化核酸例如来自含有细胞碎片的未澄清细胞裂解物的质粒的移液管尖塔和自动化方法，以及用于制备和使用这些柱的方法。 柱通常包括位于移液管末端柱中的介质床，位于底部玻璃料上方并具有可选的顶部玻璃料。

    Purification of Nucleic Acids
    9.
    发明申请
    Purification of Nucleic Acids 有权
    核酸的纯化

    公开(公告)号：US20140370590A1

    公开(公告)日：2014-12-18

    申请号：US14468286

    申请日：2014-08-25

    Applicant: Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde ,  Carrie Loan Kim Huynh

    Inventor： Chris Suh ,  Lee Hoang ,  Douglas T. Gjerde ,  Carrie Loan Kim Huynh

    IPC: C12N15/10

    CPC classification number: C12N15/101 ,  C07H1/06 ,  C07H1/08 ,  C07H21/00 ,  C12N15/1017

    Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.

    Abstract translation: 本发明解决了在生长培养基存在下从细胞中分离核酸的问题。 本发明特别可用于分离染色体外复制子如质粒。 细胞在其生长培养基的存在下裂解，并使用移液管末端柱分离核酸。 可以使用液体处理机器人同时分离多个样品中的核酸而不需要人为干预。

Patent Agency Ranking